Preparation method and application of biodegradable targeted molecule modified intelligent PLGA-SS-PEG nano-drug carrier

Abstract

The invention discloses a preparation method of a biodegradable PLGA-SS-PEG nano-drug carrier. The preparation method comprises synthesis of a PLGA-SS-PEG high-molecular polymer and preparation of PLGA nano-particles; and the PLGA nano-particles with good properties are obtained through a centrifugal method. The invention further discloses a preparation method of a chemotherapeutic drug, traditional Chinese medicine monomer or gene drug loaded targeted molecule modified intelligent response PLGA nano-drug, and application of the PLGA nano-drug in preparation of drugs for treating lung cancer,liver cancer, brain cancer or pancreatic cancer. The nano-drug can enter tumour cells through targeting and endocytosis; and the carried anti-cancer drug can be controllably released under the condition of high content of glutathione in the tumour cells, so that the purpose of inhibiting the tumour cells more efficiently with low toxicity is achieved. The diameter of the nano-drug is about 110-500nm; the blank nano-particles have excellent biocompatibility; targeted modification can achieve the purpose of specifically conveying the nano-drug to tumour cells; and the carried anti-cancer drug can be controllably released under the condition of 5-9 mM reducibility GSH.

Description

technical field [0001] The invention belongs to the research field of anti-cancer nano-medicines, in particular to a preparation method and application of anti-cancer PLGA nano-medicines modified with targeting molecules based on GSH release. Background technique [0002] Lung cancer is one of the malignant tumors with the highest morbidity and mortality. Numerous studies have identified several oncogenes, some of which may serve as therapeutic targets. One of the most successful examples is the tyrosine kinase inhibitor (TKI) of the epidermal growth factor receptor (EGFR). Previous reports have shown that aberrant expression and activation of EGFR is often associated with non-small cell lung cancer (NSCLC), which accounts for approximately 85% of all lung cancers. Therefore, EGFR can be used as a targeting molecule to specifically deliver anticancer drugs only to tumor cells. Aptamers are oligonucleotides (DNA or RNA) with high specificity and high affinity for target mo...

AI Technical Summary

Problems solved by technology

Although remarkable progress has been made in the research of smart responsive nanomedicines, most of which are based on the reports of inorganic nanomedicines, there are few studies on biodegradable organic nanomedicines, which severely restricts the development prospects of nanomedicines.

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