Compound lactic acid bacteria, fermented-alcoholized chili and preparation method of fermented-alcoholized chili
The technology of compound lactic acid bacteria and production method, which is applied in the field of agricultural product processing, can solve the problems of single seasoning, easy aging of internal structure, long fermentation time, etc., and achieves the effects of reducing production time cost, shortening production time and harmonizing flavor.
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[0036] In the present invention, the preparation method of described composite lactic acid bacteria comprises the following steps:
[0037] (1) Inoculate the slant strains of Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus respectively in the liquid medium to obtain Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus for expanded culture;
[0038] (2) The expanded cultured Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus are respectively mixed according to volume ratio to obtain composite lactic acid bacteria.
[0039] In the present invention, the inoculation adopts an inoculation loop inoculation.
[0040] In the present invention, the time for the expanded culture is independently 48-72 hours, preferably 60 hours.
[0041] In the present invention, the temperature of the expanded culture is 35-38°C, preferably 37°C.
[0042] In the present invention, the liquid medium is MRS. ...
Embodiment 1
[0111] Preparation of compound lactic acid bacteria:
[0112] The slant strains of Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus were respectively inoculated in the MRS medium with an inoculation loop, and cultivated at 37°C for 60 hours. 3000r / min, centrifuged for 15min, and centrifuged to discard the supernatant to obtain the thallines of Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus that were expanded. The bacteria were re-dissolved in sterile water, washed twice, and mixed evenly with Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus at a ratio of 1:1:2 according to the volume ratio to obtain composite lactic acid bacteria. spare.
[0113] Pretreatment of Peanut, Corn, Sesame, Garlic and Ginger:
[0114] Peanuts: baked at 180°C for 15 minutes, crushed to 0.3mm small particles after roasting to obtain crushed peanuts. spare.
[0115] Corn: choose yellow corn, peel it ...
Embodiment 2
[0127] Preparation of compound lactic acid bacteria:
[0128] The slant strains of Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus were respectively inoculated in the MRS medium with an inoculation loop, and cultured at 36°C for 72 hours. 2800r / min, centrifuged for 15min, centrifuged and discarded the supernatant to obtain the thallines of Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus for expanded culture. Dissolve the bacteria in sterile water, wash once, and mix Lactobacillus plantarum, Lactobacillus acidophilus and Streptococcus thermophilus in a ratio of 1:2:2 according to the volume ratio to obtain composite lactic acid bacteria. spare.
[0129] Pretreatment of Peanut, Corn, Sesame, Garlic and Ginger:
[0130] Peanuts: baked at 180°C for 20 minutes, crushed to 0.6mm small particles after roasting to obtain crushed peanuts. spare.
[0131] Corn: choose yellow corn, peel it, crush it to 0.5cm coarse g...
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