The invention relates to the technical field of
animal disease detection, and discloses a preparation method of a detection
reagent for
porcine circovirus type 2. The preparation method specifically comprises the following steps of S1, expression of recombinant ORF2
protein, wherein pET-28a-ORF2 is converted into
escherichia coli BL21 competent cells, the positive
escherichia coli BL21 competent cells are selected for clone and inoculated into an LB culture medium containing
kanamycin, overnight culture is performed, 50 microliters of a
bacteria solution is taken the next day to be inoculatedinto 5 milliliters of the LB culture medium containing the
kanamycin, culture is performed for 2 h until the OD600 value is 0.6-0.8, IPTG with the final concentration of 1
millimeter is added, sampling is carried out after induction is performed for 8 h, and a strain pET-28a-BL21 is induced as a
negative control, and the
protein expression situation is identified by SDS-PAGE
gel electrophoresis; S2, soluble analysis of the recombinant ORF2
protein, wherein 2 microliters of a recombined strain pET-28a-ORF2-BL21 is added into 2 milliliters of the LB culture medium containing the
kanamycin. The preparation method of the detection
reagent for the
porcine circovirus type 2 can more effectively improve the production efficiency, and can also improve the accuracy of the detection
reagent for detecting the
circovirus type 2.