Preparation method of cannabis sativa protein zymolyte craft beer

A technology of proteolysis and hemp, which is applied in the field of fermented food, can solve problems such as retention and low competitiveness, and achieve the effect of white foam, promotion of development, and pure taste

Pending Publication Date: 2020-12-22
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development of hemp protein products is still in its infancy in our country. At present, most of the

Method used

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  • Preparation method of cannabis sativa protein zymolyte craft beer
  • Preparation method of cannabis sativa protein zymolyte craft beer
  • Preparation method of cannabis sativa protein zymolyte craft beer

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specific Embodiment approach 1

[0025] Specific embodiment one: what this embodiment records is a kind of preparation method of hemp protein enzymatic hydrolyzate craft beer, described method is:

[0026] (1) Mix the hemp seed meal and distilled water according to the mass ratio of 1:10 to prepare the hemp seed meal mixture;

[0027] (2) Adjust the pH value of the hemp seed meal mixture to 5-6, add 0.5-0.8% papain, 2.5-3.5% neutral protease, and enzymolysis time 1-2 hours to obtain enzymatic hydrolysis solution; the amount of papain added 0.5-0.8% means that the quality of hemp seed meal is 100, then add 0.5-0.8 papain, 2.5-3.5% is the same;

[0028] (3) After centrifuging the enzymatic hydrolyzate in (2), take the supernatant, filter through a 3kd ultrafiltration membrane, and collect the hemp protein hydrolyzate <3kd;

[0029] (4) subjecting the hemp protein enzymatic hydrolyzate in (3) to vacuum low-temperature freeze-drying to obtain the hemp protein enzymatic hydrolyzate freeze-dried powder;

[0030] ...

specific Embodiment approach 2

[0036] Embodiment 2: In the method for preparing craft beer from hemp protein hydrolyzate described in Embodiment 1, in (4), the temperature of the low-temperature freeze-drying is -30-30° C., and the time is 24 hours.

specific Embodiment approach 3

[0037] Specific embodiment three: the preparation method of a kind of hemp protein enzymatic hydrolyzate craft beer described in specific embodiment one, in (4), the vacuum degree<10Pa.

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Abstract

The invention relates to a preparation method of cannabis sativa protein zymolyte craft beer, and belongs to the technical field of fermented food. In order to further expand the application of cannabis sativa protein, the invention provides the preparation method of the cannabis sativa protein zymolyte craft beer. The preparation method comprises the following steps of: mixing cannabis sativa seed meal with distilled water according to the mass ratio of 1:10, adjusting the pH value to 5-6, adding papain and neutral protease for enzymolysis, centrifuging enzymatic hydrolysate to obtain supernatant, collecting the cannabis sativa protein zymolyte with the concentration smaller than 3 kd, and performing vacuum low-temperature freeze drying on the zymolyte to obtain freeze-dried powder; mixing malt and water according to the mass ratio of 1:4, heating to 60-65 DEG C, carrying out heat preservation for 90 minutes, then, heating to 78-80 DEG C, and carrying out heat preservation for 20-30 minutes; and, filtering to obtain wort, adding the freeze-dried powder and humulus lupulus, boiling, filtering, cooling, adding a beer yeast, fermenting, and keeping at 4-6 DEG C for 10-15 days. According to the invention, the nutritional value of the beer is improved; furthermore, the taste and flavour of the beer are also improved; the application of the cannabis sativa seed protein in the fieldof fermented food is expanded; and the additional value of deep processing of cannabis sativa seeds is improved.

Description

technical field [0001] The invention belongs to the technical field of fermented food, and in particular relates to a method for preparing craft beer from hemp protein enzymatic hydrolyzate. Background technique [0002] Hemp (Cannabis sativa L.) is also known as industrial hemp, hemp. Hemp seed (hemp seed) is a medicine and food homologous substance with high nutritional value and health care effect. The protein content of whole hemp seeds is generally between 20% and 25%, while the protein content of the shelled seeds exceeds 40%. Hemp protein contains 21 kinds of amino acids, including 8 kinds of amino acids necessary for human body. Hemp protein is hydrolyzed by protease to generate polypeptides, which are easily digested and absorbed by the human body, and hemp seed polypeptides also have a variety of physiological functions and high nutritional value. Therefore, hemp protein hydrolyzates can be used as raw materials and additives, and are widely used in fermentation ...

Claims

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Application Information

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IPC IPC(8): C12C5/00C12C7/04C12C7/20C12C7/14C12C11/02C12R1/865
CPCC12C5/00C12C7/042C12C7/044C12C7/20C12C7/14C12C11/02
Inventor 宋淑敏石杰魏连会董艳梅雪松夏尊民高宇潘静高媛
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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