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Preparation method of rhamnose

A rhamnose and sugar solution technology, applied in the field of separation and purification of rhamnose, can solve the problems of low yield, large amount of organic solvent, and low purity of rhamnose finished products, achieve high separation and purification efficiency, and avoid production costs High, simple process effect

Inactive Publication Date: 2020-12-25
吉源(淮北)食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims at the existing technology of recovering rhamnose in the sugar liquid containing rhamnose and glucose, which has the problems of large amount of organic solvent, low purity or low yield of finished rhamnose, and provides a method without using organic solvent , and the preparation method of rhamnose with high purity and high yield

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  • Preparation method of rhamnose
  • Preparation method of rhamnose

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Embodiment 1

[0033] The present embodiment provides a kind of processing method of purifying rhamnose from glucose-containing sugar liquid, and specific processing method is as follows:

[0034] (1) Weigh 446g of pure water, heat it to 100°C and keep it warm for 30min. Weigh 358g of sugar solution, and when the pure water is naturally cooled to 52°C, dissolve the sugar solution in pure water and stir evenly to completely dissolve the sugar solution to form a reaction sugar solution. The mass percentage of glucose in the reaction sugar solution is 16 %, the sum of the mass percentages of glucose and rhamnose is 35%.

[0035] (2) Adjust the pH value of the reaction sugar solution to 5.5 with calcium hydroxide, then place the reaction sugar solution in a water bath at 52° C. and aerate the reaction sugar solution with oxygen at a rate of 1 L / min.

[0036] (3) Add 20mL of glucose oxidase (1.00g / mL, 20°C) and 20mL of catalase (1.06g / mL, 20°C) to the reaction sugar solution to obtain a reaction...

Embodiment 2

[0040] The present embodiment provides a kind of processing method of purifying rhamnose from glucose-containing sugar liquid, and specific processing method is as follows:

[0041] (1) Weigh 446g of pure water, heat it to 100°C and keep it warm for 30min. Weigh 206g of sugar solution, and when the pure water is naturally cooled to 52°C, dissolve the sugar solution in pure water and stir evenly to completely dissolve the sugar solution to form a reaction sugar solution. The mass percentage of glucose in the reaction sugar solution is 11.4 %, the sum of the mass percentages of glucose and rhamnose is 25%.

[0042] (2) Adjust the pH value of the reaction sugar solution to 5.5 with calcium hydroxide, then place the reaction sugar solution in a water bath at 52° C. and aerate the reaction sugar solution with oxygen at a rate of 1 L / min.

[0043](3) Add 16mL of glucose oxidase (1.00g / mL, 20°C) and 16mL of catalase (1.06g / mL, 20°C) to the reaction sugar solution to obtain a reactio...

Embodiment 3

[0047] The present embodiment provides a kind of processing method of purifying rhamnose from glucose-containing sugar liquid, and specific processing method is as follows:

[0048] (1) Weigh 446g of pure water, heat it to 100°C and keep it warm for 30min. Weigh 304g of sugar solution, and when the pure water is naturally cooled to 52°C, dissolve the sugar solution in pure water and stir evenly to completely dissolve the sugar solution to form a reaction sugar solution. The mass percentage of glucose in the reaction sugar solution is 14.6 %, the sum of the mass percentages of glucose and rhamnose is 32%.

[0049] (2) Adjust the pH value of the reaction sugar solution to 5.5 with calcium hydroxide, then place the reaction sugar solution in a water bath at 52° C. and aerate the reaction sugar solution with oxygen at a rate of 1 L / min.

[0050] (3) Add 19mL of glucose oxidase (1.00g / mL, 20°C) and 19mL of catalase (1.06g / mL, 20°C) to the reaction sugar solution to obtain a reacti...

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Abstract

The invention relates to the technical field of separation and purification of rhamnose, in particular to a preparation method of rhamnose. According to the method, when a sugar solution containing rhamnose and glucose is treated, glucose oxidase and catalase are used in a combined mode, glucose in the sugar solution can be effectively removed, so that rhamnose with the purity being 98% or above is obtained through separation, and the yield of rhamnose is 95% or above. Moreover, the rhamnose is separated and purified by the method disclosed by the invention, the filtrate is directly concentrated and crystallized in a water phase to obtain the white rhamnose after being decolorized, and an organic solvent is not needed, so that the high production cost caused by the use of a large amount oforganic solvents and the influence on the environment during organic solvent recovery treatment are avoided. The method is environment-friendly, simple in process and high in purification efficiency.

Description

technical field [0001] The invention relates to the technical field of separation and purification of rhamnose, in particular to a preparation method of rhamnose. Background technique [0002] Rhamnose, also known as mannose methyl sugar, is a methyl five-carbon sugar with two isomers, L-type and D-type. Pure rhamnose is a colorless crystalline powder, soluble in water and Methanol, slightly soluble in ethanol, its crystals are in two forms, α-type and β-type, α-type contains a molecule of crystal water, loses crystal water after heating, and transforms into β-type, β-type is very easy to absorb moisture and absorbs moisture in the air Transformed into α-type, the common one is α-L-rhamnose. [0003] The traditional method of producing rhamnose is to extract it from natural plants, such as extracting quercetin from oak bark, extracting naringin from citrus peel or extracting vitamin A from bark of Bechia bark. The molecules of these substances contain rhamnose groups, and ...

Claims

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Application Information

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IPC IPC(8): C07H1/06C07H3/02
CPCC07H1/06C07H3/02
Inventor 陈沁张玉武林
Owner 吉源(淮北)食品科技有限公司
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