A kind of Beauveria bassiana strain mq-08 and its application and microbial preparation
A technology of Beauveria bassiana and microbial agent, which is applied in the agricultural field and can solve the problems of Beauveria bassiana controlling coffee fruit beetles, etc.
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Embodiment 1
[0034] Embodiment 1: Beauveria bassiana MQ-08 isolation and identification
[0035] (1) Strain isolation and purification
[0036] In 2019, the coffee fruit bark beetle was collected from the coffee base of the Institute of Spices and Beverages, Chinese Academy of Tropical Agricultural Sciences, Wanning City, Hainan Province. The insects were immersed in 70% ethanol for 30 seconds and placed on a plate containing PDA medium. Cultivate in a constant temperature incubator at ℃ for 2 to 3 days. After the mycelium grows around the insect body, pick the peripheral mycelium and insert it into a new PDA plate. Repeat this for 3 to 5 times until the purified strain is obtained. The serial number is MQ-08 . Transfer the obtained purified strain MQ-08 into a slant containing PDA medium, culture at a constant temperature of 26°C for 7-15 days, and store it in a refrigerator at 4°C after a large number of conidia grow.
[0037] (2) Morphological identification
[0038] The colony textu...
Embodiment 2
[0048] Embodiment 2: the biological characteristic of Beauveria bassiana MQ-08
[0049] (1) Determination of growth rate at different temperatures
[0050] The MQ-08 strain was inoculated on a PDA plate and cultured for 15 days under five temperature conditions of 12°C, 17°C, 22°C, 26°C, and 32°C, and the colony diameter was measured once a day by the cross method. repeat. The results showed that the MQ-08 strain could grow under the five temperature conditions, and its adaptation range was wide. As the temperature increases, its growth rate is faster, reaching the fastest at 26°C, and then gradually slows down. Although the growth rate of the strain decreases slightly at 32°C, it still maintains a relatively fast growth rate, indicating that MQ-08 strain Resistant to high temperature environments.
[0051] (2) Determination of spore production
[0052] Inoculate the MQ-08 strain on a PDA plate and culture it at 26°C for 15 days. Use a hole puncher with a diameter of 0.50 ...
Embodiment 3
[0057] Example 3: Determination of the pathogenicity of Beauveria bassiana MQ-08 to the coffee berry beetle.
[0058] Inoculate the MQ-08 strain on a PDA plate, culture it at 26°C for 15 days, scrape the colony into sterile water containing 0.2% Tween-80, filter it with sterilized filter paper, calculate it with a hemocytometer, and prepare a concentration of 1 ×10 8 / mL spore suspension mother liquor, and then successively diluted to 1×10 7 , 1×10 6 , 1×10 5 , 1×10 4 per mL of spore suspension. Immerse the healthy and active adults of the coffee berry bark beetle in different concentrations of spore suspension for 10 seconds, then put them into a petri dish with sterilized filter paper at the bottom, add an appropriate amount of water to the filter paper to keep it moist, and seal it with a parafilm to prevent the worm from escaping . 10 adults per treatment, 6 repetitions, treated with 0.2% Tween-80 aqueous suspension as a control, cultured for 7 days at a temperature ...
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