Method for determining urine arsenic by electrochemical hydrogenation generation-atomic fluorescence spectrum combining technology

Abstract

The invention provides a method for determining urine arsenic by an electrochemical hydrogenation generation-atomic fluorescence spectrum combining technology. The method specifically comprises the following steps: (1) pretreating a sample; (2) preparing a standard curve; (3) judging precision and accuracy; and (4) calculating an arsenic content in urine. Compared with an existing method, the method has the advantages that use of sodium borohydride is reduced, meanwhile, a CdSO4 solution is added into a catholyte, and reduction efficiency of As is effectively improved. The method is small in reagent use amount, environmentally friendly and high in hydrogenation reduction efficiency, reagent losses can be reduced, economic benefits can be improved, and the method can be simultaneously suitable for detecting arsenic in large-batch urine and detecting low, proper and high arsenic concentrations in urine samples.

Description

technical field [0001] The invention relates to a method for measuring the content of arsenic in urine by electrochemical hydrogenation generation-atomic fluorescence method, which belongs to the technical field of chemical detection and analysis. Background technique [0002] Arsenic and its compounds are a class of toxic and harmful heavy metal pollutants, which widely exist in soil, water, and food, and are absorbed by the human body through diet, and are continuously enriched in the body. When the arsenic content in the body is too high, it will cause metal poisoning and damage Various key organs such as the heart and liver can also cause cancer and seriously affect the health of the body. Therefore, the detection result of the arsenic content in the human body is one of the important indicators for disease judgment, and it is also a key factor to measure the pollution level of human living or working environment. The detection of arsenic content in the human body usuall...

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Problems solved by technology

[0003] The existing methods for the determination of arsenic content in urine mainly include spectrophotometry, atomic absorption method, atomic fluorescence method and ICP-MS, etc. Among them, the hydrogenation-atomic fluorescence method has the advantages of low instrument cost, simple sample pretreatment, and high sensitivity. The most commonly used method for the determination of arsenic; however, this method requires the use of acidic borohydride as a reducing agent, which will decompose and produce gaseous hydride during the detection process, causing reagent contamination and affecting the detection results; meanwhile, existing methods use The fluorescence signal of arsenic measured by the advanced atomic fluorescence spectrometer is weak, and the detection results are not obvious enough, which hinders the further popularization and application of this method.

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