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Quantum dot bead having multifunctional ligand, and target antigen detection method and bio-diagnostic apparatus using same

A detection method, quantum dot technology, applied in disease diagnosis, biomaterial analysis, chemical instruments and methods, etc., can solve the problems of applying diagnostic equipment, increasing surface area, difficult bead complexes, etc., to achieve amplified detection intensity and improved detection sensitivity , fast and simple detection and identification of the effect

Pending Publication Date: 2021-01-08
ZEUS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] However, the limitation of the bead complex is that it increases the surface area of ​​the beads and the sensitivity enhancement through fluorescent substance accumulation requires a separate washing procedure for each step, and thus it is difficult to apply the bead complex to in situ diagnostic devices.

Method used

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  • Quantum dot bead having multifunctional ligand, and target antigen detection method and bio-diagnostic apparatus using same
  • Quantum dot bead having multifunctional ligand, and target antigen detection method and bio-diagnostic apparatus using same
  • Quantum dot bead having multifunctional ligand, and target antigen detection method and bio-diagnostic apparatus using same

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0096] [Preparation Example 1] Preparation of quantum dots having biotin on their surface

[0097] (1) Preparation of oil-soluble quantum dots

[0098] In a 3-neck flask, 1.0 g of zinc acetate (Zn(Ac) 2 ), 0.441 g of cadmium oxide (CdO), 20 mL of oleic acid, and 75 mL of octadecene (ODE) were mixed and at 150° C. for 1 hour under a nitrogen atmosphere to remove water. Subsequently, the resulting flask was heated to 300° C., and then 1 mL of trioctyl (TOP) and 0.045 g of selenium (Se) were injected and heated for 3 minutes, whereby quantum dot cores were formed.

[0099] Then, 0.5 mL of dodecanethiol was added to the 3-necked flask and reacted for 10 minutes. Then, a solution containing 1 mL of TOP and 0.025 g of sulfur (S) was added to the reaction vessel of the 3-necked flask and reacted for 20 minutes, whereby a shell was formed. Then, the resulting core and shell were purified with a mixed solution of ethanol and toluene and dissolved in an organic solvent, whereby first...

preparation Embodiment 2

[0109] [Preparation Example 2] Preparation of quantum dot beads having a multifunctional ligand bound to avidin

[0110] (1) Synthesis and surface modification of silica particle substrate

[0111] Silica-based nanoparticles were synthesized by the Stober method. First, the NH 4 OH, EtOH, and H 2 O was stirred in a Erlenmeyer flask at a ratio of 3:60:1 mL, 2 mL of tetraethylorthosilicate (TEOS) was added to the reactant, and the mixture was stirred and reacted at 50° C. for 18 hours or more. Here, the reaction time and mixing ratio can be adjusted according to the desired size. Subsequently, final samples were obtained by centrifugation using ethanol. Herein, silica beads of about 200 nm can be obtained.

[0112] Subsequently, for the reaction between the surface and the quantum dots, as reactive functional groups, 180 μL of 3-mercaptopropyltrimethoxysilane (MPTS) and NH 4 OH and react for 12 to 24 hours. After purification by centrifugation using ethanol, a surface-mod...

experiment Embodiment 1

[0127] [Experimental Example 1] Confirmation of Characteristics of Quantum Dots and Quantum Dot Beads

[0128] (1) Zeta potential of quantum dots and quantum efficiency of quantum dots and quantum dot beads

[0129] The zeta potentials of the quantum dots of Preparation Examples 1-(2) and 1-(3) were measured using ELS-100ZS (Otsuka Corp.), and at figure 2 The results are shown in .

[0130] The quantum efficiencies of the quantum dots of Preparation Example 1-(2) and the quantum dot beads of Preparation Example 2-(3) were measured using QE 2000 (Otsuka Corp.), and image 3 The results are shown in . According to these results, according to one aspect of the present disclosure, the quantum dots and quantum dot beads prepared in Preparation Examples 1-(2) and 2-(3) exhibited quantum dots of 92±3% and 83±3%, respectively. Efficiency and since both are greater than 80%, it shows an excellent effect.

[0131] (2) Confirmation of the size and shape of quantum dots and quantum do...

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Abstract

In one aspect, the present disclosure relates to a quantum dot bead comprising a multifunctional ligand having a first conjugated material and a second antibody, and an immunochromatographic detectionmethod for a target antigen in a biological sample, comprising forming multiple bonds with a quantum dot having a second conjugated material. In addition, the present disclosure has the effect of remarkably amplifying the detection intensity and significantly improving the detection sensitivity without a separate washing step, and thus enables the detection and diagnosis of physiological substances in a biological sample even in an actual product, and may be used to provide a product with excellent price competitiveness.

Description

technical field [0001] The present disclosure relates to quantum dot beads with multifunctional ligands and methods and biodiagnostic devices using the same to detect target antigens. Background technique [0002] In recent years, as the focus of medicine has shifted from treatment to diagnosis and from centralized hospital diagnosis to on-site and individualized diagnosis, there is a need for diagnostic equipment that can directly make a diagnosis on-site and measure many types of diseases in the simplest manner. In order to realize such a device, the three most necessary factors may include high sensitivity of the diagnostic device, suitable price, and availability of various diagnoses, and in order to achieve these factors, various diagnostic platforms have been studied. [0003] Currently, the most common methods in in vitro diagnostics are protein-based (protein-based) assays, such as immunoassays, or nucleic acid-based (nucleic acid-based) molecular diagnostic techniqu...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/558B01L3/00
CPCG01N33/558B01L3/00B01J20/0262B01J20/3204B01J20/0244B01J20/28007B01J20/0266B01J20/3219B01J20/3293B01J20/289B01J20/3274B01J20/3085B01J20/103G01N33/588G01N33/54346B01L3/5023G01N2800/00B01L2300/0825G01N33/54386G01N21/6428G01N2021/6441B01J2220/54G01N33/543G01N33/54388G01N33/54389G01N33/553
Inventor 丁兴琇申圣荣金贤洙朴相绚李智英
Owner ZEUS
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