Combined feed additive for palm meal type feed as well as preparation method and application of combined feed additive
A feed additive and palm meal technology, which is applied in the field of combined feed additives and the preparation of combined feed additives, can solve the problems of high cost, unfavorable promotion and application of small feed enterprises and farms, low feed utilization rate, etc. Activity, improve overall utilization, targeted effect
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Embodiment 1
[0042] The invention provides a combined feed additive for palm meal feed, which consists of 34-48 parts by mass of compound enzyme preparation, 6.8-11.2 parts by mass of compound microorganisms and 40.8-59.2 parts by mass of multi-species solid fermentation product ;
[0043] Wherein, the compound enzyme preparation consists of 8-12 parts by mass of β-mannanase with 50000U / g enzyme activity, 3-5 parts by mass of xylanase with 100000U / g enzyme activity, 15-19 parts by mass with 7000U / g Enzyme active α-amylase, 3-5 mass parts of 50000U / g enzyme activity acid protease and 5-7 mass parts of 50000U / g enzyme activity neutral protease, the xylanase is composed of yeast xylan Enzyme and Trichoderma xylanase are mixed in a ratio of 7:3;
[0044] The multi-species solid fermentation product is prepared by solid-state fermentation of a substrate, 100 billion viable bacteria / g bacillus subtilis and 10 billion viable bacteria / g bacillus coagulans.
[0045] In this example, the additives...
Embodiment 2
[0072] The present embodiment provides a kind of preparation method for the combination type feed additive of palm meal type feed, and its purpose is to manufacture 500kg for the combination type feed additive of palm meal type feed, comprises the following steps:
[0073] S1. Compound enzyme preparation preparation: Weigh 53kg of β-mannanase with 50000U / g activity, 18kg of xylanase with 100000U / g activity, 80kg of α-amylase with 7000U / g activity, 80kg of 50000U / g The acid protease 20kg of g enzyme activity, the neutral protease 28kg of 50000U / g enzyme activity, drop into mixer successively, mix 3 minutes;
[0074] S2. Compound microbial preparation: Weigh 5.5kg of Bacillus subtilis with 100 billion / g viable bacteria, 18kg of Enterococcus faecalis with 5 billion / g viable bacteria, and Bacillus coagulans with 10 billion / g viable bacteria 21kg, put into the drum mixer one by one, mix for 3 minutes;
[0075] S3, preparation of multi-strain solid fermentation product:
[0076] S...
experiment example 3
[0083] This experimental example is used to measure the enzymatic properties of yeast-type xylanase and Trichoderma-type xylanase mixed into combined type xylanase in the ratio of 7:3 in the present invention, and respectively with separate yeast The comparison of the enzymatic characteristics of the type xylanase and the Trichoderma type xylanase illustrates the advantages of the combination type xylanase, specifically:
[0084] Weigh 70 g of 100,000 U / g yeast-type xylanase and 30 g of 100,000 U / g Trichoderma-type xylanase respectively, and mix them uniformly to become combined type xylanase, which is ready for use.
[0085] Using the method stipulated in "GB / T 23874-2009 Determination of Feed Additive Xylanase Activity Spectrophotometric Method", the yeast type xylanase, Trichoderma type xylanase and combination type xylanase were respectively measured in For the enzyme activities in water baths with different pH and different temperatures, the highest enzyme activity was ta...
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