Application of plasma protein composition in preparation of product for predicting low-dose radiation irradiation dose

A radiation exposure and low-dose technology, applied in the application field of products, can solve the problems that low-dose radiation dose and damage prediction cannot be applied

Pending Publication Date: 2021-01-22
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing medium and high dose radiation dose and damage pred...

Method used

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  • Application of plasma protein composition in preparation of product for predicting low-dose radiation irradiation dose
  • Application of plasma protein composition in preparation of product for predicting low-dose radiation irradiation dose
  • Application of plasma protein composition in preparation of product for predicting low-dose radiation irradiation dose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Example 1. Antibody chip detection of changes in plasma protein content after long-term multiple low-dose radiation

[0151] C57BL / 6 mice: from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., male, weighing 18-22 g.

[0152] 12 C57BL / 6 mice were grouped according to Table 1 and carried out 60 Coγ-ray irradiation (cobalt source, Institute of Radiation Medicine, Academy of Military Medicine, Academy of Military Sciences). All mice were 24 hours after the last irradiation, blood was collected from the heart, and the plasma was extracted. Three mice were taken from each group, and antibody chip technology (Raybiotech, USA, article number: AAM-BLG-1) was used for analysis and detection according to the instructions of the chip.

[0153] Table 1. Irradiation scheme for mice in low-dose irradiation protein chip detection experiment

[0154]

[0155] After irradiation in each group, the results of antibody chip analysis of plasma protein signal intensity c...

Embodiment 2

[0157] Example 2, ELISA detection of changes in plasma protein content after long-term multiple low-dose radiation

[0158] According to the results of Example 1, IL-5, IL-10, IL-12p40, MMP14, SAA1 and SELP were selected as detection objects, and ELISA kits were used to detect the changes in the levels of these proteins in plasma after long-term multiple low-dose radiation.

[0159] 40 C57BL / 6 mice were grouped according to Table 2 and carried out 60 Co gamma-ray irradiation. All mice were 24 hours after the last irradiation, blood was collected from the heart, and the plasma was extracted. Eight mice per group were used to detect the expression levels of IL-5, IL-10, IL-12p40, MMP14, SAA1 and SELP in plasma using ELISA kits. The source of the ELISA kit (Wuhan Elabscience Company), was detected according to the instructions of the ELISA kit.

[0160] Table 2. Mouse irradiation scheme for plasma protein ELISA detection after low-dose irradiation of mice

[0161]

[0162]...

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Abstract

The invention discloses application of a plasma protein composition in preparation of a product for predicting a low-dose radiation irradiation dose. The invention provides the application of a substance for detecting IL-5, IL-12p40 and/or SELP content in blood plasma. The application comprises the following steps of: preparing the product for predicting the low-dose radiation irradiation dose; and preparing products for predicting the number and/or duration of low dose radiation irradiation. The plasma protein composition can be used as an index for predicting the long-term low-dose radiationaccumulated dose, and then the application of the plasma protein composition in predicting the long-term low-dose radiation accumulated dose is provided. The application has important significance indiagnosing the radiation accumulated dose of a radiological worker threatened by long-term low-dose radiation.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of plasma protein combination in the preparation of products for predicting low-dose radiation exposure. Background technique [0002] The impact of long-term low-dose radiation on human health has always been a concern of people. Professionals engaged in medical X-ray diagnosis, interventional therapy, radiotherapy, nuclear medicine, nuclear industry, nuclear mining and industrial flaw detection, etc., during long-term low-dose exposure, The blood, nerves, digestive system, skin, eye lens, and genetics are susceptible to radiation to varying degrees, but the specific affected tissues, parts, and degree of damage are still controversial. Studies have shown that long-term low-dose radiation can lead to low resistance and low spirits in humans or animals, and the clinical symptoms of exposed subjects are related to low-dose exposure doses. For example, in a study, 490 o...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6869G01N33/6893G01N33/543G01N2800/40
Inventor 付汉江葛常辉郑晓飞朱捷
Owner ACADEMY OF MILITARY MEDICAL SCI
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