Zymophyte liquid for whole-plant fermentation of hybrid broussonetia papyrifera and preparation method and application of zymophyte liquid

A fermentation method and fermentation bacteria technology, applied in the field of agriculture, can solve the problems of difficult large-scale industrial application, incomplete fermentation degree, and low utilization, so as to promote decomposition and utilization, improve meat quality and flavor, and promote the decomposition of cellulose Effect

Inactive Publication Date: 2021-01-26
中商构能生态科技(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current fermentation technology of hybrid mulberry tree is limited to the fermentation of its fresh branches and leaves, and the degree of fermentation is not complete, the utilization is low, and the fermentation has little effect on the tall stems with relatively high lignification degree.
At the same time, the work of picking fresh and tender branches and leaves must be done manually, with low efficiency and high cost, and it is difficult to carry out large-scale industrial application. There is an urgent need for a fermentation and utilization technology that can be applied to all stems and branches of hybrid trees. Picking is mechanized harvesting, and all harvested products are fully fermented and utilized to improve efficiency and reduce costs

Method used

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  • Zymophyte liquid for whole-plant fermentation of hybrid broussonetia papyrifera and preparation method and application of zymophyte liquid
  • Zymophyte liquid for whole-plant fermentation of hybrid broussonetia papyrifera and preparation method and application of zymophyte liquid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation of embodiment 1 fermentation liquid

[0028] 1.1 Take sterilized pure water, add brown sugar 7%, white sugar 5%, fructose 2%, honey 2% by weight, then add Pichia pastoris 95000cfu / ml, Torula yeast 80000cfu / ml, Lactobacillus acidophilus 90000cfu / ml ml, Bifidobacterium 70000cfu / ml, Lactococcus lactis 60000cfu / ml, Lactobacillus casei 80000cfu / ml, mix well, place on a shaker, anaerobic culture at 38°C for 15 days and measure daily.

[0029] When the number of Lactococcus lactis colonies is greater than 1,000,000 cfu / ml, the fermentation is stopped to obtain a fermentation stock solution.

[0030] Before use, the fermentation stock solution is diluted 8 times with sterile water to obtain a fermentation solution.

[0031] 1.2 Take sterilized pure water, add brown sugar 5%, white sugar 3%, fructose 1%, honey 5% by weight, then add Pichia pastoris 90000cfu / ml, Torula yeast 75000cfu / ml, Lactobacillus acidophilus 80000cfu / ml ml, Bifidobacterium 60000cfu / ml, Lact...

Embodiment 2

[0037] Embodiment 2 handles hybrid tree

[0038] 2.1 Fresh processing

[0039] The whole hybrid mulberry plant is freshly harvested, minced and fermented directly.

[0040] 2.2 Green storage processing

[0041] The whole hybrid mulberry plant is freshly harvested, crushed, and then fermented after a period of green storage.

[0042] 2.3 Drying treatment

[0043] The whole plant of the hybrid mulberry tree is harvested and dried to less than 10% of the water content, minced and then fermented.

Embodiment 3

[0044] The whole plant fermentation of embodiment 3 hybrid mulberry

[0045] 3.1 For fresh hybrid trees

[0046] Add 5% fermentation liquid and 1% brown sugar to the freshly harvested hybrid mulberry, put it in an airtight container, and ferment for 15 days at 35-40°C;

[0047] Derivative fermentation: Add 10% fermentation broth and 1% brown sugar to the freshly harvested hybrid mulberry tree, and add 1-10% corn flour, 1-10% soybean flour, 1-10% soybean meal powder, and place in a closed In the container, ferment for 15 days at 35-40°C.

[0048] 3.2 For silage hybrid trees

[0049] Add 20% fermentation broth and 2% brown sugar to the hybrid mulberry tree treated with green storage, put it in a closed container, and ferment for 15 days at 35-40°C;

[0050] Derivative fermentation: Add 15% fermentation broth and 2% white sugar to the hybrid mulberry tree treated with green storage, and add 1-10% corn flour, 1-10% soybean flour, 1-10% soybean meal powder, and place in In a cl...

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Abstract

The invention belongs to the technical field of agriculture, and particularly relates to zymophyte liquid for whole-plant fermentation of hybrid broussonetia papyrifera and a preparation method and application of the zymophyte liquid. The zymophyte liquid for whole-plant fermentation of hybrid broussonetia papyrifera is prepared by mixing fermenting of probiotics such as pichia pastoris, torula, lactobacillus acidophilus and bifidobacterium with saccharides. The zymophyte liquid can effectively degrade lignin and cellulose in the broussonetia papyrifera.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a fermented bacterium liquid used for fermenting the whole plant of hybrid mulberry tree and its preparation method and application. Background technique [0002] The crude protein content of hybrid mulberry is as high as 26% to 32%, rich in various amino acids, vitamins, trace elements and flavonoid physiologically active substances, and the leaves of hybrid mulberry are thicker than wild mulberry. However, the current fermentation technology of hybrid mulberry tree is limited to the fermentation of its fresh and tender branches and leaves, and the degree of fermentation is not complete, the utilization is low, and the fermentation has almost no effect on the high stems with relatively high lignification degree. At the same time, the work of picking fresh and tender branches and leaves must be done manually, with low efficiency and high cost, and it is difficult ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K50/30A23K10/12C12R1/84C12R1/88C12R1/23C12R1/245C12R1/01
CPCC12N1/20C12N1/16A23K50/30A23K10/12A23V2400/125A23V2400/113A23V2400/231A23V2400/51
Inventor 许标
Owner 中商构能生态科技(天津)有限公司
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