Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Complex amplification kit for supplementing STR site for difficult detection materials and application thereof

A compound amplification and kit technology, which is applied in the field of molecular biology, can solve the problems of large sub-fragments, weak anti-inhibitor ability, and low sensitivity, and achieve the effects of short amplified fragments, improved utilization, and high-efficiency detection

Pending Publication Date: 2021-01-26
AGCU SCIENTECH
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing conventional kits have large amplicon fragments, low sensitivity, and weak anti-inhibitor ability, which cannot meet the detection requirements of difficult samples

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Complex amplification kit for supplementing STR site for difficult detection materials and application thereof
  • Complex amplification kit for supplementing STR site for difficult detection materials and application thereof
  • Complex amplification kit for supplementing STR site for difficult detection materials and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The kit of the present invention consists of the following: primer mixture, 1mL; genomic DNA, 10ng; PCR buffer Master Mix, 2mL; sdH 2 O, 1.95 mL; PCR enhancer.

[0033] The composition of the PCR buffer Master Mix is: hot start Taq enzyme 0.2U / μL~0.5U / μL, Tris-HCl 50mM~125mM with pH 8.25~8.4, dNTPs 5.0mM~7.5mM, KCl 50mM~125mM, BSA 2mg / ml~5mg / ml.

[0034] The optional types of PCR enhancers are: gelatin, polyethylene glycol, dimethyl sulfoxide, Triton X-100, or dithiothreitol. Choose one or two of them and add them directly to the PCR buffer Master Mix.

[0035] 1. The operation steps are as follows:

[0036] 1.1 Amplification system

[0037] Component volume Master Mix 10.0 μL extract sample 6μL The primer described in claim 1 5.0 μL wxya 2 o

Make up to 25 μL

[0038] 1.2 The amplification procedure is:

[0039]

[0040] 1.3. Fluorescent detection of the amplified product on a genetic analyzer

[0041] Centrifuge ...

Embodiment 2

[0045] A total of 469 samples of blood stains, saliva spots, exfoliated cells, and old degraded samples in the actual case were tested using the kit of the present invention and the similar kit EX25 developed by our company respectively.

[0046] The kit of the present invention consists of the following: primer mixture, 1mL; genomic DNA, 10ng; PCR buffer Master Mix, 2mL; sdH 2 O, 1.95 mL; PCR enhancer.

[0047] The composition of the PCR buffer Master Mix is: hot start Taq enzyme 0.2U / μL~0.5U / μL, Tris-HCl 50mM~125mM with pH 8.25~8.4, dNTPs 5.0mM~7.5mM, KCl 50mM~125mM, BSA 2mg / ml~5mg / ml.

[0048] The optional types of PCR enhancers are: gelatin, polyethylene glycol, dimethyl sulfoxide, Triton X-100, or dithiothreitol. Choose one or two of them and add them directly to the PCR buffer Master Mix.

[0049] 1. Sample preparation: extract by magnetic bead method, refer to "GA / T 383-2002 Forensic Science DNA Laboratory Inspection Specification" for specific methods.

[0050] 2. T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a complex amplification kit for supplementing STR site for difficult detection materials and application thereof. The kit comprises 17 pairs of specific amplification primers of STR loci, wherein the STR loci are Amelogenin, D3S1358, D6S477, D1S1656, D19S253, D2S441, D6S1043, D15S659, D3S3045, Penta E, D8S1132, Penta D, D10S1435, D22S1045, D10S1248, D12S391 and DYS391. Thekit has the advantages of rapid and efficient detection of the difficult detection materials such as trace, degradation and inhibitor-containing materials in a field case; and meanwhile, the kit has high efficiency for amplification of the difficult case detection materials, and the optimized locus information can supplement the existing case database, shorten the case investigation time and lockthe suspect.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to gene detection by using molecular markers, in particular to a supplementary STR site compound amplification kit for difficult test materials and its application. Background technique [0002] The short tandem repeat locus (short tandem repeat, STR) was first proposed in the early 1990s, and its core sequence generally consists of 2-6 bases, and the common one is 4 bases. Length polymorphism is present due to differences in the number of core sequence repeats between different individuals and between different loci. The fragment amplicon of the gene locus is relatively small, and multiple STR loci can also be amplified multiple times. Compared with RFLP and other technologies, it can obtain rich information of multiple loci at the same time. Sensitive, accurate and other obvious advantages. [0003] Due to the above-mentioned obvious advantages, the complex STR-DNA typing technolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6879C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6879C12Q1/6888C12Q1/686C12Q2600/156C12Q2563/107Y02A50/30
Inventor 邵泽香刘煜尧范晓芸姜颖晔王伟陈林丽夏子芳郑卫国
Owner AGCU SCIENTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com