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Bacteriostatic anti-aging vagina-cleaning nursing wet tissue and preparation method thereof

A technology of cleansing yin and wet wipes, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of only cleaning and disinfection functions, single function, etc., to promote long-term health and stability, promote youthful state, Infectious effect

Inactive Publication Date: 2021-01-29
黄新华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Feminine care wipes currently on the market often have a single function, only cleaning and disinfecting

Method used

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  • Bacteriostatic anti-aging vagina-cleaning nursing wet tissue and preparation method thereof
  • Bacteriostatic anti-aging vagina-cleaning nursing wet tissue and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of the fermentation extract of Lactobacillus pentosus 10# is:

[0041] (1) Strain activation:

[0042] A Lactobacillus cyclopentosus 10# was inoculated into MRS solid medium and streaked, and cultured anaerobically at 37°C for 24 h. Pick a single colony with good growth on the plate and inoculate it into MRS liquid medium for activation, and culture it anaerobically to 1*10 8 cfu / ml Lactobacillus pentosus 10# seed solution, use lactic acid to adjust the pH value to 5.0.

[0043] (2) Configure fermentation medium

[0044] Lactobacillus pentosus 10# seed liquid was inoculated into the MRS liquid medium containing 0.5% arborvitin A according to the inoculum size 10% v / v%, and cultured on a shaking table at 37° C. at 200 rpm for 24 hours to obtain a fermentation liquid;

[0045] (3) Preparation of fermentation extract:

[0046] Centrifuge the fermented liquid obtained in step (2) at a rotational speed of 6000r / min for 40min, collect the supernatant,...

Embodiment 2

[0048] The fermentation extract of Example 1 was dissolved in serum-free DMEM culture fluid, and 5 samples were prepared with mass fractions of 0.5%, 1%, 5%, 10%, and 20%, respectively, and serum-free DMEM culture fluid was used as a negative control Sample, 20% DMSO is positive control sample. Three replicates were set up for each sample. The survival rate of cells in each group relative to the positive control group was detected by MTT method. The experimental results are shown in Table 1:

[0049] Table 1 Cell survival rate of different concentration extracts

[0050]

[0051] The relative survival rate of cells in the experimental group was higher than that of the positive control group, indicating that the lower dose of fermentation extract had no effect on cell growth and could also stimulate cell proliferation;

Embodiment 4

[0059] The antioxidant capacity of Example 1 and Comparative Examples 1-4 were compared.

[0060] According to the conventional methods in this field, VC (vitamin C) was selected as the positive control in this example, and the Example 1 group, Comparative Example 1-4 group and V C Group.

[0061] Each group was diluted with pure water respectively, and prepared into sample solutions with mass fractions of 0.5%, 1%, 1.5%, 2%, and 2.5%, respectively, adding samples, FeSO4·7H2O solution ( 4.5mmol / L), salicylic acid-ethanol solution and, H 2 o 2 solution (4.4 mmol / L), and then mix well, incubate in a constant temperature water bath at 37°C for 30 min in the dark, and measure the absorbance at 510 nm;

[0062] Hydroxyl radical scavenging rate (%)=[1-(A1-A2) / A0]×100%

[0063] A0: 1.0ml FeSO4·7H2O+1.0 ml salicylic acid+1.0 ml hydrogen peroxide+1.0 mLH2O

[0064] A1: 1.0ml FeSO4·7H2O+1.0ml salicylic acid+1.0ml hydrogen peroxide+1.0mL sample

[0065] A2: 1.0ml FeSO4·7H2O+1.0 ml ...

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Abstract

The invention discloses a bacteriostatic anti-aging vagina-cleaning nursing wet tissue which is characterized in that the wet tissue is prepared by injecting the following raw material components intospunlace non-woven fabric: a fermentation extracting solution of lactobacillus pentosus 10 # and other necessary auxiliary agents. Preferably, platycladus orientalis glycoside A is added into a fermentation medium of the fermentation extracting solution of the lactobacillus pentosus 10 #. The natural lactobacillus from food is selected and used, safety and reliability are achieved, harmful bacteria can be inhibited without adding a bactericide, and the problem that the bactericide influences the balance of vagina flora is avoided. The lactobacillus fermentation extracting solution can help tomaintain the balance of the vagina flora, and promote the long-term health and stability of the vagina, and the vagina is not easy to infect. The wet tissue prepared by the invention also has a remarkable anti-aging effect, and can effectively maintain the vagina part skin and promote the young state of the vagina part skin.

Description

technical field [0001] The invention relates to the application of probiotic technology in the field of female care, and more particularly to an antibacterial and anti-aging cleansing wet tissue and a production method thereof. Background technique [0002] Wet wipes are moist paper towels used to wipe the skin. Wet wipes on the market can be roughly divided into two categories: one has been sterilized itself, but cannot sterilize other items. It contains skin care ingredients and can only be used for skin moisturizing and maintenance. The other is a disinfectant wipe that is not only disinfected itself, but also disinfects other items. It can be used for disinfection or sterilization of skin abrasions, scratches, etc. [0003] Women's nursing wipes are wet wipes for women's private parts. Generally speaking, they contain disinfectant ingredients, which can suppress harmful bacteria such as E. coli and Staphylococcus aureus. Nursing wipes for women are suitable for menstru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9794A61K8/9789A61K8/60A61K8/34A61K8/02A61P15/00A61P15/02A61Q19/00A61Q19/08
CPCA61K8/0208A61K8/345A61K8/602A61K8/99A61K2800/85A61Q19/00A61Q19/08A61K8/9789A61K8/9794A61P15/00A61P15/02
Inventor 黄新华
Owner 黄新华
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