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Preparation method of bionic artificial temporomandibular joint disc

A temporomandibular joint, artificial technology, applied in the field of preparation of bionic artificial temporomandibular joint discs, can solve problems such as difficult reconstruction, poor stability, and inability to match the physiological load of temporomandibular joints, and achieve low preparation cost, low cost and excellent preparation method Convenient and fast effect

Active Publication Date: 2021-01-29
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a preparation method of a bionic artificial temporomandibular joint disc, which utilizes a decellularization method to remove the immunogenicity of the protozoan articular disc, and constructs a brand new bionic artificial temporomandibular joint The disk can not only retain the composition and structure of the temporomandibular joint disk to the greatest extent, but also realize the stability of the shape of the joint disk, and the preparation method is convenient and fast, and the preparation cost is low, which effectively solves the problem that the existing technology cannot match the temporomandibular joint. Physiological load, poor stability, and difficulty in reconstruction, etc., are easy to promote and use

Method used

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  • Preparation method of bionic artificial temporomandibular joint disc

Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] A method for preparing a bionic artificial temporomandibular joint disc, comprising the following steps:

[0025] (1) Collect the temporomandibular joint disc of a miniature pig with a body weight of 35kg under aseptic conditions, wash it with penicillin saline to remove blood stains, and then use a 17W American light-wave ultraviolet laser to roughen the pores to obtain sample 1;

[0026] (2) Place the sample 1 obtained in step (1) in the mixed solution for 36 hours under the condition of a shaker at 3°C, then centrifuge to extract glycosaminoglycans and proteins, and then freeze-dry the remaining supernatant, and the treated The temporomandibular joint discs were collected for later use to obtain sample 2; the mixed solution was prepared by the following method: adding protease inhibitors to 3M guanidine hydrochloride internal solution, and mixed well to obtain a mixed solution; the volume ratio of guanidine hydrochloride internal solution and protease inhibitor was 40...

Embodiment 2

[0030] A method for preparing a bionic artificial temporomandibular joint disc, comprising the following steps:

[0031] (1) Collect the temporomandibular joint disc of a minipig with a body weight of 40kg under aseptic conditions, wash it with penicillin saline to remove blood stains, and then use a 17W American light-wave ultraviolet laser to roughen the pores to obtain sample 1;

[0032] (2) Place the sample 1 obtained in step (1) in the mixed solution for 48 hours under the condition of a shaker at 4°C, then centrifuge to extract glycosaminoglycans and proteins, and then freeze-dry the remaining supernatant, and the treated The temporomandibular joint disk was collected for later use to obtain sample 2; the mixed solution was prepared by the following method: adding protease inhibitors to 4M guanidine hydrochloride internal solution, and mixing to obtain a mixed solution; the volume ratio of guanidine hydrochloride internal solution and protease inhibitor was 450 :1;

[0...

Embodiment 3

[0036] A method for preparing a bionic artificial temporomandibular joint disc, comprising the following steps:

[0037] (1) Collect the temporomandibular joint disc of a minipig with a body weight of 45kg under aseptic conditions, wash it with penicillin saline to remove blood stains, and then use a 17W American light-wave ultraviolet laser to roughen the pores to obtain sample 1;

[0038] (2) Place the sample 1 obtained in step (1) in the mixed solution for 60 hours under the condition of a shaker at 5°C, then centrifuge to extract glycosaminoglycans and proteins, then freeze-dry the remaining supernatant, and put the treated The temporomandibular joint disks were collected for later use to obtain sample 2; the mixed solution was prepared by the following method: adding protease inhibitors to 5M guanidine hydrochloride internal solution, and mixing evenly to obtain a mixed solution; the volume ratio of guanidine hydrochloride internal solution and protease inhibitor was 500 ...

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Abstract

The invention provides a preparation method of a bionic artificial temporomandibular joint disc, which comprises the following steps: collecting a temporomandibular joint disc of a pig, performing cleaning, and coarsening pores to obtain a sample I; placing the sample I in the mixed solution, then carrying out centrifuging and freeze-drying, and collecting a temporomandibular joint disc to obtaina sample II; soaking and stirring the sample II with a lauryl sodium sulfate solution and DNA enzyme to obtain an acellular temporomandibular joint disc; blending glycosaminoglycan and protein with polycaprolactone, polyvinyl alcohol, methacryloyl gelatin and P respectively, then subjecting the mixture and the acellular temporomandibular joint disc to perfusion, and obtaining the bionic artificialtemporomandibular joint disc. Immunogenicity of an original animal articular disc is removed through a decellularization method, and the problems that in the prior art, physiological loads of temporomandibular joints cannot be matched, stability is poor, and reconstruction is difficult are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a bionic artificial temporomandibular joint disc. Background technique [0002] Temporomandibular joint disk (Temporomandibular joint disk, TMJ disk) is a fibrocartilage-like tissue located between the mandibular condyle and the glenoid fossa of the temporal bone. Distributes loads, cushions shocks, and reduces dissonance between condyle and glenoid bony structures. Clinically, the destruction or loss of the temporomandibular joint disc is one of the main causes of temporomandibular joint diseases, and it will also cause abnormal function of the entire stomatognathic system, seriously affecting the quality of life of patients. According to relevant data, there are as many as one million patients in the world who need to reconstruct the articular disc every year, and the medical expenses required are also as high as hundreds of mil...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50A61L27/56
CPCA61L27/3612A61L27/3654A61L27/3687A61L27/3691A61L27/50A61L27/56A61L2400/18A61L2430/24
Inventor 姜楠祝颂松陈浩哲王旻张洁
Owner SICHUAN UNIV
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