Production method of anti-mildew and anti-sticking feed
A production method and feed technology applied in the field of anti-mildew and anti-sticking feed, which can solve the problems of energy consumption, loss of fermented soybean meal, and increased cost
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Embodiment 1
[0053] The site was selected for trial operation in Hunan Liangping Biotechnology Co., Ltd., Changsha City, Hunan Province. The soybean meal raw material was obtained from Hunan Liangping Agriculture and Animal Husbandry Development Co., Ltd. Specific steps are as follows:
[0054] S1, preparation bacterial enzyme, concrete operation is as follows:
[0055] 1) Obtain the required fermentation strains: Take 1g each of Baiyunbian distillery koji, vegetable garden soil, and Baiyunbian distillery pit mud in a 100mL Erlenmeyer flask, add PDA medium for enrichment and culture for 12 hours, dilute and spread, pick For a single colony, add 1% milk powder or 1% soluble starch to the plate, and screen the strains with transparent circles around the colonies, which are strains with high protease or amylase production respectively, and its 28ITS DNA is verified as Aspergillus oryzae (MQ) by PCR amplification and sequencing , Aspergillus niger (HQ), yeast (YT) strains.
[0056] 2) Prepar...
Embodiment 2
[0089] The site was selected for trial operation in Hubei Liangping Biological Fermentation Technology Co., Ltd. The raw material of soybean meal was taken from Hubei Liangping Biological Fermentation Technology Co., Ltd. Specific steps are as follows:
[0090] S1, preparation bacterial enzyme, concrete operation is as follows:
[0091] 1) Obtain the required fermentation strains: Take 1g each of Baiyunbian distillery koji, vegetable garden soil, and Baiyunbian distillery pit mud in a 100mL Erlenmeyer flask, add PDA medium for enrichment and culture for 12 hours, dilute and spread, pick For a single colony, add 1% milk powder or 1% soluble starch to the plate, and screen the strains with transparent circles around the colonies, which are strains with high protease or amylase production respectively, and its 28ITS DNA is verified as Aspergillus oryzae (MQ) by PCR amplification and sequencing , Aspergillus niger (HQ), yeast (YT) strains.
[0092] 2) Prepare the medium required...
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