Method for extracting 1-deoxynojirimycin from fresh mulberry leaves

A technology of deoxynojirimycin and fresh mulberry leaves, applied in the direction of organic chemistry, etc., can solve the problems of difficult large-scale production and high production cost, and achieve the effects of increasing pH value, increasing yield, and improving dissolution efficiency

Pending Publication Date: 2021-02-02
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese invention patent CN109180563A discloses a method for extracting DNJ from fresh mulberry leaves. Although a high-content DNJ extract is obtained by this method, the process r

Method used

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  • Method for extracting 1-deoxynojirimycin from fresh mulberry leaves
  • Method for extracting 1-deoxynojirimycin from fresh mulberry leaves
  • Method for extracting 1-deoxynojirimycin from fresh mulberry leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Get 10kg of fresh mulberry leaves, clean, drain, and cut into strips with a diameter of 3mm; the mulberry leaves after the strips are added into 60L of 70°C, 5% acetic acid extract, and ultrasonic (frequency 40KHz, sound intensity 0.6 W / cm 2 ,) assisted extraction for 30min; then use 60L of pure water to extract twice with the same ultrasonic wave, each time for 20min; combine the extracts, filter with a diatomaceous earth filter, and concentrate in vacuo; dilute the concentrate with purified water to a specific gravity of 1.15, Stand still at 0-4°C for 72 hours; take the supernatant, filter it through an 8 μm filter membrane, pass it through a cation exchange resin, wash it with water until it is colorless, and then elute it with an alkaline aqueous solution until it is colorless, and collect the alkaline eluent; The eluate was concentrated and dried to obtain extract 1.

Embodiment 2

[0030] Get 10kg of fresh mulberry leaves, clean, drain, and cut into strips with a diameter of 4mm; the mulberry leaves after the strips are added into 70L of 60°C extract containing 10% acetic acid, and ultrasonic (frequency 100KHz, sound intensity 1W) / cm 2 ,) assisted extraction for 30min; then use 70L of pure water to extract twice with the same ultrasonic wave, each time for 20min; combine the extracts, filter with a diatomaceous earth filter, and concentrate in vacuo; dilute the concentrate with purified water to a specific gravity of 1.19, Stand still at 0-4°C for 72 hours; take the supernatant, filter it through a 20 μm filter membrane and pass it through a cation exchange resin, then wash it with water until it is colorless, then elute it with an alkaline aqueous solution until it is colorless, and collect the alkaline eluent; The eluate was concentrated and dried to obtain extract 2.

Embodiment 3

[0032] Get 10kg of fresh mulberry leaves, clean, drain, and cut into strips with a diameter of 6mm; add 80L of mulberry leaves with a temperature of 50°C and an extract containing 20% ​​acetic acid, and use ultrasonic waves (frequency 200KHz, sound intensity 2W / cm 2 ,) assisted extraction for 30 minutes; then use 80L of pure water to assist extraction twice with the same ultrasonic wave, each time for 20 minutes; combine the extracts, filter with a plate and frame filter, and concentrate in vacuum; dilute the concentrate with purified water to a specific gravity of 1.22, Stand still at 0-4°C for 72 hours; take the supernatant, filter it through a 60 μm filter membrane and pass it through a cation exchange resin, then wash it with water until it is colorless, then elute it with an alkaline aqueous solution until it is colorless, and collect the alkaline eluent; The eluate was concentrated and dried to obtain extract 3.

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Abstract

The invention discloses a method for extracting 1-deoxynojirimycin from fresh mulberry leaves. The method comprises the following steps: cleaning and draining fresh picked mulberry leaves; cutting thedrained fresh mulberry leaves into strips, and putting the mulberry leaves into an extracting solution containing acetic acid; recovering an extracting solution after ultrasonic treatment; carrying out ultrasonic-assisted extraction on the mulberry leaves twice by taking purified water as an extraction solvent; combining extracting solutions, filtering, concentrating in vacuum, refrigerating, filtering, concentrating and drying to obtain an extract. The method does not need a large amount of organic solvents, and is simple in process, easy to operate and high in extraction rate. The obtainedextract can be used for preparing high-purity 1-deoxynojirimycin.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, in particular to a method for extracting 1-deoxynojirimycin from fresh mulberry leaves. Background technique [0002] Mulberry leaves, also known as iron fan, are the dried leaves of the Moraceae plant Sang (Morus alba L.), which is a traditional Chinese medicine. Traditional Chinese medicine believes that mulberry leaves are cold in nature and sweet in taste, and have the functions of dispelling wind-heat, clearing liver and improving eyesight, cooling blood to stop bleeding, clearing lung and moistening dryness. Mulberry leaf is approved by the Ministry of Health as a dual-use medicine and food. It is rich in resources in my country and has great potential for development. [0003] Studies have found that mulberry leaves contain a special hypoglycemic substance, namely 1-deoxynojirimycin (1-deoxynojirimycin, DNJ). DNJ can competitively inhibit the activity of small intestin...

Claims

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Application Information

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IPC IPC(8): C07D211/46
CPCC07D211/46
Inventor 董志超何际婵
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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