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A kind of green and environmental protection process for preparing 2-amino-3-substituted phenyl-3-hydroxypropionic acid

A hydroxypropionic acid, green and environmentally friendly technology, applied in biochemical equipment and methods, lyase, fermentation, etc.

Active Publication Date: 2022-07-05
杭州濡湜生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the industrial biosynthesis method of 2-amino-3-substituted phenyl-3-hydroxypropionic acid. The present invention adopts bio-enzyme catalysis technology combined with separation and fixed bed to design a new process of environmental protection, high efficiency and energy saving, filling The blank of industrial biosynthesis of this kind of product

Method used

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  • A kind of green and environmental protection process for preparing 2-amino-3-substituted phenyl-3-hydroxypropionic acid
  • A kind of green and environmental protection process for preparing 2-amino-3-substituted phenyl-3-hydroxypropionic acid
  • A kind of green and environmental protection process for preparing 2-amino-3-substituted phenyl-3-hydroxypropionic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Add 300L activated macroporous adsorption resin D301 (resin D301) to 1500L of aldolase-containing enzyme solution (phosphate buffer concentration is 0.5M, pH is 7.2, enzyme content is 38mg / L, specific enzyme activity is 14000U / mg). The density is about 0.7g / ml), stirred at 5~10℃ for 13h, then the macroporous adsorption resin was filtered and washed with 500L purified water to obtain the immobilized aldolase resin. The phosphate buffer solution obtained after filtration is repeatedly used for immobilization as the solvent of the enzyme solution, and the purified water after washing is repeatedly used to wash the macroporous adsorption resin after filtration.

[0036] 2. Dissolve 150kg of glycine into 3800kg of pure water, raise the temperature to 40±1°C (pre-heating, saving production time), add 222kg of p-methylsulfonylbenzaldehyde, 78g of ZnCl after complete dissolution 2 , stir evenly, adjust the pH to 7.5±0.2, then add 70L of the immobilized aldolase resin prepare...

Embodiment 2

[0041] 1. Add 300L activated macroporous adsorption resin D301 (resin D301) to 1500L of aldolase-containing enzyme solution (phosphate buffer concentration is 0.5M, pH is 7.2, enzyme content is 38mg / L, specific enzyme activity is 14000U / mg). The density is about 0.7g / ml), stirred at 5~10℃ for 13h, then the macroporous adsorption resin was filtered and washed with 500L purified water to obtain the immobilized aldolase resin. The phosphate buffer solution obtained after filtration is repeatedly used for immobilization as the solvent of the enzyme solution, and the purified water after washing is repeatedly used to wash the macroporous adsorption resin after filtration.

[0042] 2. Dissolve 280kg glycine into 3800kg pure water, the temperature rises to 40±1°C (pre-heating, saving production time), add 170kg p-chlorobenzaldehyde, 78g ZnCl after complete dissolution 2 , stir evenly, adjust the PH to 7.5±0.2, then add 70L of the immobilized aldolase resin prepared in step 1, and sti...

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Abstract

The invention discloses a green environmental protection process for preparing 2-amino-3-substituted phenyl-3-hydroxypropionic acid, using immobilized aldolase as a biological catalyst, substituted benzaldehyde and glycine as substrates, and divalent metal ions as Enzymatic agent, water as solvent, PH 6~9, temperature 35~65℃, stirring speed 50~200rpm, to carry out enzymatic catalysis. The excess raw materials and products are separated by a separation bed containing a specific complex resin, and the divalent metal ion enzymatic agent in the reaction is completely removed. The glycine solution can be recovered and used mechanically. The 2-amino-3-substituted phenyl-3- Hydroxypropionic acid is subjected to subsequent concentration, crystallization and drying to obtain the product, and the water obtained by concentration can also be recovered and applied mechanically. Through the biological enzyme catalysis technology of aldolase, the immobilized aldolase, the separation bed of a specific complex resin and the effective combination between them, a set of green and environmental protection production technology with self-circulation of solvent materials and no three wastes is formed. .

Description

technical field [0001] The invention relates to the technical field of compound preparation, in particular to a green environmental protection process for preparing 2-amino-3-substituted phenyl-3-hydroxypropionic acid. Background technique [0002] 2-Amino-3-substituted phenyl-3-hydroxypropionic acid is an important pharmaceutical intermediate and chemical intermediate, especially 2-amino-3-(4-methylsulfonylphenyl)-3-hydroxypropionic acid Acid and 2-amino-3-(4-chlorophenyl)-3-hydroxypropionic acid, widely used in the preparation of human antibiotics, poultry and livestock antibiotics, etc., its representative drugs such as chloramphenicol, thiamphenicol, fluorobenzene Niko and more. [0003] The current mainstream of 2-amino-3-substituted phenyl-3-hydroxypropionic acid synthesis is chemical synthesis. Although the chemical synthesis method is simple to operate, a large amount of water, heavy metals and highly toxic hydrogen sulfide are used in the reaction process, and the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/04C12N9/88C12N11/082
CPCC12P13/04C12N9/88C12N11/082Y02P20/54
Inventor 王喆明谭昊
Owner 杭州濡湜生物科技有限公司