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Bacillus thuringiensis suspending agent and preparation method thereof

A Bacillus thuringiensis and suspending agent technology, applied in the field of microorganisms, can solve the problems of shortening the duration of the drug effect of the Bacillus thuringiensis suspension, reducing the insecticidal effect, inactivating the insecticidal crystal protein, etc., so as to prolong the duration of the drug effect and improve the killing effect. Insect effect, good insecticidal effect

Pending Publication Date: 2021-02-05
SHANDONG LUKANG BIOLOGICAL PESTICIDE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When adopting the Bacillus thuringiensis suspension in the above technical scheme, since the main component of the Bacillus thuringiensis suspension for killing insects is the insecticidal crystal protein, the insecticidal crystal protein is easily inactivated by the influence of ultraviolet rays, so that the drug effect of the Bacillus thuringiensis suspension continues period shortens, resulting in reduced insecticidal efficacy

Method used

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  • Bacillus thuringiensis suspending agent and preparation method thereof
  • Bacillus thuringiensis suspending agent and preparation method thereof

Examples

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Effect test

preparation example 1

[0061] A Bacillus thuringiensis fermented liquid, comprising the steps of:

[0062] (1) Preparation of seeds

[0063] Take 0.1mL of Bacillus thuringiensis with a sterile pipette, drop it on the first medium, spread it evenly with an inoculation needle, and then put it into a biochemical incubator for cultivation at a temperature of 30.0±1.0°C for 72 hours to obtain seeds for production , wherein the first medium is a slant medium;

[0064] (2) Preparation of strain suspension

[0065] Add the prepared seeds and 150mL sterile saline into the eggplant bottle together, scrape the slant seeds on the slant medium into the 250mL eggplant bottle with an inoculation needle to make a suspension; put the eggplant bottle at 80°C Heat in a water bath for 20 minutes;

[0066] (3) Fermentation tank culture

[0067] ① Medium configuration: prepare liquid medium, sterilize, cool down and wait for inoculation;

[0068] ②Inoculation: Inoculate the strains of each eggplant bottle into 1.4 t...

preparation example 2-4

[0074] The difference between Preparation Examples 2-4 and Preparation Example 1 is that the process parameters of each step are different, and the specific data are shown in Table 1.

[0075] Table 1

[0076]

preparation example 5

[0078] The difference between this preparation example and preparation example 4 is that both bean cake powder and peanut cake powder are crushed to 90 mesh.

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Abstract

The invention discloses a bacillus thuringiensis suspending agent and a preparation method thereof. The bacillus thuringiensis suspending agent comprises the following components in parts by weight: 6to 13 parts of capsule core, 4 to 8 parts of oil liquid, 10 to 21 parts of a wall material mixed solution, and 0.04 to 0.08 part of an emulsifying agent; the capsule core is prepared by adding whitecarbon black into bacillus thuringiensis fermentation liquor, adsorbing the bacillus thuringiensis fermentation liquor by the white carbon black, filtering moisture and then drying at 35 to 39 DEG C;the weight part ratio of the bacillus thuringiensis fermentation liquor to the white carbon black is (105 to 116): 1.5. The capsule wall is adopted to coat the bacillus thuringiensis insecticidal active substance in the capsule core, so that the bacillus thuringiensis insecticidal active substance is not easily affected by ultraviolet rays to be inactivated, and the purposes of prolonging the pesticide effect duration of the bacillus thuringiensis suspension and improving the insecticidal effect are achieved. The invention further provides a preparation method of the bacillus thuringiensis suspension. The preparation method has the advantage of simple process.

Description

technical field [0001] The application belongs to the technical field of microbes, and more specifically, it relates to a Bacillus thuringiensis suspension and a preparation method thereof. Background technique [0002] Bacillus thuringiensis preparation is the most widely used biopesticide in the world. It has the characteristics of high efficiency and low toxicity, and has made great contributions to the production of green food and the protection of human living environment. [0003] In the related art, a suspension of Bacillus thuringiensis is proposed, including 100 parts of Bacillus thuringiensis fermentation liquid, 6 parts of phosphate ester, 3 parts of nonylphenol polyoxyethylene ether, 0.2 part of xanthan gum, 3 parts of ethylene glycol, 0.1g Silicone defoamer, 100 parts deionized water. [0004] When adopting the Bacillus thuringiensis suspension in the above technical scheme, since the main component of the Bacillus thuringiensis suspension for killing insects i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/23A01N47/30A01P7/04
CPCA01N47/30A01N63/23
Inventor 韩慧培张坤李兴聂桂芬魏淑秀
Owner SHANDONG LUKANG BIOLOGICAL PESTICIDE CO LTD
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