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Lactobacillus rhamnosus capable of intervening in metabolic syndrome and application

A technology of Lactobacillus rhamnosus and metabolic syndrome, applied in the field of microbiology, can solve the problem of the lack of research on probiotics in the colon intestinal barrier, and achieve the restoration of intestinal flora imbalance, maintenance of intestinal barrier, and improvement of excessive weight gain. Effect

Active Publication Date: 2021-02-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above patents all use probiotics to relieve metabolic syndrome, and there are few studies on the probiotics of Lactobacillus rhamnosus in the intervention and prevention of metabolic syndrome and how to protect the intestinal barrier of the colon

Method used

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  • Lactobacillus rhamnosus capable of intervening in metabolic syndrome and application
  • Lactobacillus rhamnosus capable of intervening in metabolic syndrome and application
  • Lactobacillus rhamnosus capable of intervening in metabolic syndrome and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Example 1: Lactobacillus rhamnosus CCFM1146 has good tolerance to simulated gastrointestinal fluid

[0087] Streak-inoculate the cryopreserved Lactobacillus rhamnosus CCFM1146 in MRS solid medium (MRS medium + 0.05% cysteine ​​hydrochloride), anaerobic static culture at 37°C for 48h, and then MRS culture After two or three subcultures, take 1ml of the culture solution of Lactobacillus rhamnosus CCFM1146, mix it with 9.0mL pH 2.5 artificial simulated gastric juice (MRS medium containing 1% pepsin, pH = 2.5), and incubate at 37°C. Under anaerobic culture, samples were taken at 0h, 0.5h, 1h and 2h, and MRS agar medium was poured for culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate. The survival rate is the ratio of the number of viable bacteria when sampling in the culture solution to the number of viable bacteria at the 0th hour, expressed in %.

[0088] Take 1ml of the culture solution of Lactobacillus rhamnosus ...

Embodiment 2

[0093] Example 2: Lactobacillus rhamnosus CCFM1146 has no toxic side effects on C57BL / 6J mice

[0094] The cells of Lactobacillus rhamnosus CCFM1146 were resuspended in normal saline to make a concentration of 1×10 10 CFU / mL bacterial suspension. Eight healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, 0.2 ml of the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0095] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 1×10 10 CFU / mL of Lactobacillus rhamnosus CCFM1146 had no significant impact on mice, no significant change in body weight, no obvious pathological symptoms in appearance, and no death.

[0096] Table 3 Changes in body weight and death of mice

[0097] time (days) 1 2 3 4 5 6 7 weight(g) 20.42±0.5 20.59±0.4 20.71...

Embodiment 3

[0099] Example 3: Lactobacillus rhamnosus CCFM1146 has a recovery effect on intestinal flora imbalance caused by high-fat feed

[0100] Forty-eight healthy male C57BL / 6J mice weighing 19-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (Simvastatin control group). ), metformin control group (Metformin), Lactobacillus rhamnosus CCFM1146 intervention group (CCFM1146), Lactobacillus rhamnosus FZJJH6L2 intervention group control group (FZJJH6L2), each group contained 8 mice. Lactobacillus rhamnosus FZJJH6L2 is a strain screened from fresh stool samples of healthy people in Jinhua City, Zhejiang Province according to the same screening steps as Lactobacillus rhamnosus CCFM1146, and its 16S rDNA sequence is shown in SEQ ID NO.2.

[0101] Mix the bacteria slime and protective agent (100g / L-150g / L skimmed milk powder, 100g / L-150g / L maltodextrin, 140g / L-160...

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Abstract

The invention discloses lactobacillus rhamnosus capable of intervening in metabolic syndrome and an application, in particular to a strain capable of intervening in metabolic syndrome. The lactobacillus rhamnosus can be added into various healthy foods and health-care foods, and belongs to the technical field of microorganisms. The invention provides lactobacillus rhamnosus CCFM1146 as well as a composition, a fermented food, an application, a microbial agent and a microbial agent preparation method thereof. According to the lactobacillus rhamnosus CCFM1146, the metabolic syndrome caused by high-fat diet are remarkably improved; the blood glucose, glucose tolerance and insulin resistance index level of metabolic syndrome mice are remarkably improved; the abundance of microorganisms in intestinal tracts is remarkably improved, and a colon barrier structure is maintained; and the level of proinflammatory factor IL-6 in liver is remarkably improved. The lactobacillus rhamnosus CCFM1146 isused for preparing pharmaceutical compositions and the fermented food for relieving the metabolic syndrome, non-alcoholic fatty liver disease, diabetes and other diseases, and has a very wide application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus rhamnosus capable of interfering with metabolic syndrome and its application, especially a strain capable of interfering with metabolic syndrome, which can be added to various health foods and health foods, and belongs to the technical field of microorganisms. Background technique [0002] Metabolic syndrome (MS) is a group of central obesity, hyperglycemia (diabetes or impaired glucose regulation), dyslipidemia (including high TG and / or low HDL-C blood), and hypertension. At the same time, there are multiple risk factors based on abnormal glucose metabolism and lipid metabolism that gather together to promote the occurrence and development of various cardiovascular and cerebrovascular diseases such as type II diabetes and atherosclerosis. In recent years, with the continuous development of my country's economic and social level, the living standards of the public have continued to improve, the proport...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/747A61P1/00A61P3/10A61P3/06A61P1/16A61P29/00A61P9/12A61P3/04A23C9/123A23C13/16A23C19/032A23L19/20A23L33/135C12R1/225
CPCA61K35/747A61P1/00A61P3/10A61P3/06A61P1/16A61P29/00A61P9/12A61P3/04A23C9/1234A23C13/16A23C19/0323A23L19/20A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2200/30A23V2200/328A23V2200/3262A23V2200/3204A23V2200/326A23V2200/332
Inventor 杨波陈卫郑富莉赵建新张灏
Owner JIANGNAN UNIV
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