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A kind of separation and purification method of adenosylmethionine

A technology for the separation and purification of adenosylmethionine, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of limited application, easy inactivation reaction, poor stability of adenosylmethionine, etc., and achieve improved dissolution rate , high precipitation rate, and reduce the effect of impurity removal steps

Active Publication Date: 2021-12-03
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the most critical problems in the production and application of adenosylmethionine are poor stability and prone to inactivation reactions, which largely limit its clinical application. It has been found that preparing it into a salt can to a certain extent Improves the stability of adenosylmethionine

Method used

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  • A kind of separation and purification method of adenosylmethionine

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Embodiment 1

[0039] Embodiment 1 A kind of separation and purification method of adenosylmethionine

[0040] Include the following steps:

[0041] S1, wall breaking treatment: get 5mL of fermented liquid and carry out centrifugation, the centrifugation rate is 6000r / min, remove the supernatant, after washing, centrifuge again with the speed of 6000r / min to remove the supernatant, take the precipitate and add 6mL volume ratio 5: 1 solution of 0.1mol / L acetic acid and 0.6mol / L sulfuric acid, shake at room temperature for 60 minutes, centrifuge at a rate of 6000r / min, filter through a 0.22μm filter membrane, and keep the supernatant for later use;

[0042] S2, adjust the pH value of the supernatant obtained in step S1 to be 3, and then pass the mixed weakly acidic ion exchange resin with a volume ratio of 2:1 of D113 resin and D115 resin in series, using a concentration of 0.25mol / L of sulfuric acid to The flow velocity of 2BV / h is eluted, obtains adenosylmethionine sulfuric acid eluate;

...

Embodiment 2

[0044] Embodiment 2 A kind of separation and purification method of adenosylmethionine

[0045] Include the following steps:

[0046] S1, wall breaking treatment: get 5mL of fermented liquid and carry out centrifugation, the centrifugation rate is 8000r / min, remove the supernatant, after washing, centrifuge again with the speed of 8000r / min to remove the supernatant, take the precipitate and add 5mL volume ratio 10: 1 solution of 0.3mol / L acetic acid and 1.0mol / L sulfuric acid, shake at room temperature for 30 minutes, centrifuge at a rate of 8000r / min, filter through a 0.22μm filter membrane, and keep the supernatant for later use;

[0047] S2, adjust the pH value of the supernatant obtained in step S1 to be 5, and then pass the mixed weakly acidic ion exchange resin with a volume ratio of 3:1 of D113 resin and D115 resin in series, using a concentration of 0.3mol / L of sulfuric acid to The flow velocity of 3BV / h is eluted, obtains adenosylmethionine sulfuric acid eluate;

...

Embodiment 3

[0049] Embodiment 3 A kind of separation and purification method of adenosylmethionine

[0050] Include the following steps:

[0051] S1, wall breaking treatment: take 5 mL of fermented liquid and carry out centrifugation, the centrifugation rate is 7000r / min, remove the supernatant, after washing, centrifuge again with the speed of 7000r / min to remove the supernatant, take the precipitate and add 9mL volume ratio 8: 1 solution of 0.2mol / L acetic acid and 0.8mol / L sulfuric acid, shake at room temperature for 50 minutes, centrifuge at a rate of 7000r / min, filter through a 0.22μm filter membrane, and keep the supernatant for later use;

[0052] S2, adjust the pH value of the supernatant obtained in step S1 to be 4, and then pass the mixed weakly acidic ion exchange resin with a volume ratio of 3:1 of D113 resin and D115 resin in series, using a concentration of sulfuric acid of 0.25mol / L to The flow rate of 2.5BV / h is eluted to obtain ademetionine sulfuric acid eluate;

[0053...

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Abstract

The invention provides a method for separating and purifying adenosylmethionine, which relates to the technical field of bioengineering. The method comprises the following steps: S1, cell wall breaking; S2, column purification; S3, crystallization purification; in step S1, the solvent is added acetic acid and sulfuric acid with a volume ratio of 5‑10:1 during the wall breaking process, which can effectively ensure the dissolution of small molecules, The macromolecules in the cells are intercepted, thereby effectively reducing the impurity removal steps in the subsequent treatment, thereby increasing the yield of adenosylmethionine. In the column purification process, a mixed large-pore resin is used, and the volume of the large-pore resin is controlled. Compared with that, impurities can be effectively removed, so that the obtained adenosylmethionine has high purity. The method for separating and purifying adenosylmethionine provided by the present invention has a yield of more than 80% and a purity of more than 99.5% for the finally obtained adenosylmethionine.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for separating and purifying adenosylmethionine. Background technique [0002] Adenosylmethionine, also known as adenosylmethionine, is the active form of methionine, and its structure is: Discovered in 1951, it widely exists in animals, plants and microorganisms. It is also an important physiologically active substance in the human body and participates in more than 40 biochemical reactions. In organisms, methionine and ATP are catalyzed and synthesized by adenosylmethionine synthase. In this reaction, the adenosine of ATP is transferred to methionine to form a high-energy sulfur atom, which activates the carbon atom connected to it through nucleophilic attack, thus having Transmethylation, transaminopropyl, transthiol, etc. Adenosylmethionine is an important physiologically active substance related to the metabolism of fat, protein, sugar, etc. in the human b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/167C07H1/06
CPCC07H1/06C07H19/167
Inventor 周彦乐马婷侯争平王奇刘强
Owner 内蒙古拜克生物有限公司
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