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Simultaneous detection of six active ingredients in Chuanbei Qingfei Syrup by HPLC

A technology of active ingredients and Qingfei syrup, which is applied in the field of HPLC method for simultaneous detection of six active ingredients in Chuanbei Qingfei syrup, can solve problems such as the difficulty in comprehensively controlling the quality of traditional Chinese medicine preparations, and achieve improved peak shape, resolution, and separation The effect of good degree and easy operation

Active Publication Date: 2022-06-07
HUBEI WUDANG JINDING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the content determination of active ingredients is one of the key contents in the quality standards of traditional Chinese medicine. For compound preparations, it is difficult to be objective, comprehensive and sensitive only for a single component or a few components in the preparation. It is difficult to fully control the quality of traditional Chinese medicine preparations if it reflects the quality of Chuanbei Qingfei Syrup preparation

Method used

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  • Simultaneous detection of six active ingredients in Chuanbei Qingfei Syrup by HPLC
  • Simultaneous detection of six active ingredients in Chuanbei Qingfei Syrup by HPLC
  • Simultaneous detection of six active ingredients in Chuanbei Qingfei Syrup by HPLC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 The HPLC method that simultaneously detects 6 kinds of active ingredients in Chuanbei Qingfei Syrup

[0054] Specific steps are as follows:

[0055] S1. Precisely measure 10ml sample of Chuanbei Qingfei Syrup to be tested, place it in a conical flask with a sealed stopper, add 50ml of 30% ethanol aqueous solution, accurately weigh it after sealing, and ultrasonically treat it at 55-60°C (frequency 40KHz, Power 200W, treatment time 25min) and then place to cool, supplement with 30% ethanol aqueous solution to reduce weight loss, shake well and filter with 0.45μm microporous membrane, take the filtrate to obtain the test solution;

[0056] S2. Add 15 μL of triethylamine and 85 μL of 0.4 mg / L benzoyl chloride / diethyl ether solution to the obtained solution of the test product, and after derivatization reaction at 35 ° C for 30 min, inject it into a high performance liquid chromatograph, and test by HPLC method After obtaining the chromatogram;

[0057] The c...

Embodiment 2

[0067] Embodiment 2 The HPLC method that simultaneously detects 6 kinds of active ingredients in Chuanbei Qingfei Syrup

[0068] The HPLC method of detecting 6 kinds of active ingredients in Chuanbei Qingfei Syrup provided by the present embodiment has the same steps as those in Example 1, and the difference is only:

[0069] The mobile phase gradient elution parameters are:

[0070]

[0071] Compared with Example 1, both the resolution and sensitivity between the chromatographic peaks of the active ingredient decreased in Example 2; specifically, the sensitivity (based on the minimum detection limit of quantification) decreased by 4.8-8.7%.

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Abstract

The invention provides an HPLC method for simultaneously detecting 6 active ingredients in Chuanbei Qingfei Syrup. The 6 active ingredients include ursolic acid, oleanolic acid, amygdalin, catalpol, glycyrrhizic acid and liquiritin; the steps include: Measure the sample of Chuanbei Qingfei Syrup to be tested, extract it after adding an aqueous alcohol solution, and obtain the test solution; inject it into a high-performance liquid chromatograph, and obtain a chromatogram after using the HPLC method for testing, wherein mobile phase A and mobile phase B are acetonitrile and 0.08‑0.12% phosphoric acid aqueous solution respectively; the contents of six active ingredients in Chuanbei Qingfei Syrup were calculated by external standard method. The invention improves the separation degree between chromatographic peaks by optimizing the mobile phase and gradient elution mode, and the detection method has high sensitivity; by using benzoyl chloride derivatization reagent for derivatization, the sensitivity can be improved, and the low abundance can be accurately and effectively detected quantitatively. active ingredient; the method has fast detection speed, high sensitivity, high precision and good repeatability.

Description

technical field [0001] The invention relates to the technical field of detection of active pharmaceutical ingredients, more particularly, to an HPLC method for simultaneously detecting six active ingredients in Chuanbei Qingfei Syrup. Background technique [0002] Cough is a common clinical disease, but it is rarely cured quickly. At present, neither oral chemical drugs nor infusions can cure coughs completely and quickly; in comparison, traditional Chinese medicine preparations have few side effects, even if the medication cycle is slightly longer (7-10 days), no side effects will appear, so the advantages of traditional Chinese medicine are significantly reflected. . [0003] Chuanbei Qingfei Syrup is derived from the classic prescription in "Treatise on Febrile Diseases and Miscellaneous Diseases" written by medical sage Zhang Zhongjing. It has the functions of clearing the lungs and moistening dryness, relieving cough and resolving phlegm, and is used for the treatment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 黄正军何德苟肖作武王世才陈昊
Owner HUBEI WUDANG JINDING PHARMA