Bisphenol S and lead combined pollution biological model and establishment method thereof
A technology of biological model and establishment method, applied in general water supply conservation, animal husbandry, etc., can solve the problems of strict cultivation environment requirements, high cost, and many consumables, and achieve the effect of easy cultivation, low cost and fast reproduction
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0028] (1) Preparation of Caenorhabditis elegans in L1 stage:
[0029] The configuration of NGM medium: Weigh 3g of sodium chloride and 2.5g of tryptone into a 1L beaker, add 1L of distilled water and stir evenly to fully dissolve the sodium chloride and tryptone, and then evenly distribute it into four 500mL cones Conical flasks, each containing 250mL of solution. Since the agar powder is difficult to dissolve at low temperature, after the prepared solution is divided into packages, 4.25g of agar powder is weighed and added to four conical flasks, and finally sterilized under high pressure at 121°C for 30 minutes. After autoclaving, cool to 65°C, and add 1M calcium chloride and magnesium sulfate solution, 5mg / mL cholesterol solution 250μL, 1M 6.25 mL of potassium phosphate buffer.
[0030] Configuration of M9: Weigh 3g of potassium dihydrogen phosphate, 6g of disodium hydrogen phosphate, 5g of sodium chloride, 1mL of 1M magnesium sulfate in a 1L beaker, add 1L of distilled ...
Embodiment 2
[0038] In this example, the extent of the lethal effect on C. elegans of co-contamination with bisphenol S and lead was determined.
[0039] (1) Caenorhabditis elegans LC under single substance exposure 50 Determination of:
[0040] Bisphenol S solution: Dissolve BPS with ethanol to obtain 1M BPS mother solution, dilute with M9 to obtain 2.0 mM, 2.5 mM, 3.0 mM, 3.5 mM, 4.0 mM, 5.0 mM, and the final ethanol concentration of each concentration is 0.1%.
[0041] Lead solution: Dissolve lead in deionized water to obtain 1mM lead mother solution, dilute to 500μM, 300μM, 250μM, 200μM, 125μM, 67.5μM with deionized water.
[0042] Preparation of S base solution: use 50mL K +Dissolve 5.8g of sodium chloride in the solution (1M), adjust the pH to 6.0, add 950mL of distilled water and 1.0mL of cholesterol solution (5mg / ml) and mix well, then autoclave at 121°C for 30min, cool to room temperature, and set aside.
[0043] K + Preparation of solution (1M): Weigh 10.83g potassium dihydro...
Embodiment 3
[0059] In this example, the Caenorhabditis elegans models exposed to different concentrations were randomly picked with a platinum wire needle, and by measuring the head swinging frequency and body bending frequency of Caenorhabditis elegans, comparing the difference between the actual value and the theoretical value to verify the effect on double Motility of Caenorhabditis elegans under combined pollution with phenol S and lead. Among them, the theoretical value is the superposition of the determination of the corresponding indicators of Caenorhabditis elegans under the exposure of a single substance.
[0060] (1) Head swing frequency
[0061] Add 20-30 μL of M9 buffer dropwise to the glass slide cleaned with alcohol to form a small water droplet, pick up a Caenorhabditis elegans after acute exposure into the small water droplet with a platinum wire needle, and recover for 1 minute . Observe the number of swings of the C. elegans head from side to side under a stereomicrosc...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More