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Application of piroxicam in preparation of medicine for treating skin ulcer and preparation method of medicine

A technology for piroxicam and skin ulcers, which is applied in the application of piroxicam in the preparation of medicines for treating skin ulcers and the field of medicine preparation, and achieves the effects of promoting wound healing and promoting migration

Pending Publication Date: 2021-02-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is not effective for patients with complete occlusion of the arteries of the affected limbs or severe arterial stenosis
At present, there are no relevant studies and reports on its use in the treatment of skin ulcers

Method used

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  • Application of piroxicam in preparation of medicine for treating skin ulcer and preparation method of medicine
  • Application of piroxicam in preparation of medicine for treating skin ulcer and preparation method of medicine
  • Application of piroxicam in preparation of medicine for treating skin ulcer and preparation method of medicine

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0023] Take the ointment as an example to illustrate its preparation method, comprising the following steps:

[0024] Step 1: dissolving piroxicam in PEG400 to form mixed solution A;

[0025] Step 2: PEG8000 and water are fully dissolved to form a mixed solution B, and the mass ratio of PEG8000 and water in the mixed solution B is 2:1;

[0026] Step 3: Mix the mixed solution A and the mixed solution B thoroughly to form the mixed solution C, seal it, let it stand overnight at 4°C, and solidify into a paste to obtain the desired drug;

[0027] The mass ratio of PEG 400, PEG 8000 and water in the mixed solution C is 2:2:1; the concentration of piroxicam in the mixed solution C is 0.1mol / L-0.15mol / L.

Embodiment 1

[0029] Because one of the pathological mechanisms of diabetic foot ulcer is local hypoxia, human umbilical vein endothelial cells (Human Umbilical Vein Endothelial Cells, HUVEC) were selected in this embodiment. Human umbilical vein endothelial cells were cultured under high-glucose and low-serum conditions for 24 hours to simulate the vascular ischemic state caused by diabetes, and the CCK-8 cell proliferation activity test, scratch test, and tube formation test were used to detect the conditions of high-glucose and low-serum conditions. Cell migration negligence of human umbilical vein endothelial hebamine under 24h and under normal conditions for 24h.

[0030] Follow the steps below:

[0031] Human umbilical vein endothelial cells HUVEC were cultured in high-glucose DMEM medium containing 10% fetal bovine serum (FBS) at 37°C, 5% CO 2 Subculture in an incubator. When the cells reached 90% confluency, they were subcultured on the cell culture plate, randomly divided into th...

Embodiment 2

[0038] First prepare piroxicam ointment according to the following steps:

[0039] Step 1: Dissolve 30mg of piroxicam in 3.4ml of PEG400 to form mixed solution A;

[0040] Step 2: Fully mix PEG 8000 and water according to the mass ratio of 2:1 to form 5.1ml mixed solution B;

[0041] Step 3: Mix the mixed solution A and the mixed solution B thoroughly, pour it into a flat-bottomed open container, seal it, and let it stand overnight at 4°C, and it can be used after it solidifies into a paste.

[0042] The blank control ointment preparation process is as follows:

[0043] Step 1: Dissolve 20g PEG 8000 in 10ml water to form mixed solution A;

[0044] Step 2: Mix 20ml PEG 400 with the mixed solution A in step 1 thoroughly, pour it into a flat-bottomed open container, and seal it; let it stand overnight at 4°C, and use it after it solidifies into a paste.

[0045] Establishment of Rat Skin Ulcer Model

[0046] 8-week-old C57BL / 6 male rats were selected for weighing, and randoml...

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Abstract

The invention discloses application of piroxicam in preparation of a medicine for treating skin ulcer and a preparation method of the medicine. The medicine is prepared from piroxicam and pharmaceutically acceptable auxiliary materials. The piroxicam can promote migration of human umbilical vein endothelial cells (HUVEC cells) under high glucose stimulation, so that healing of diabetic foot ulcerwounds is promoted; and the piroxicam has a remarkable curative effect on skin ulcer, especially diabetic foot ulcer.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the application of piroxicam in the preparation of medicines for treating skin ulcers and the preparation method of the medicines. Background technique [0002] Skin ulcer is common disease, frequently-occurring disease, and clinical manifestation skin ulcer can not heal for a long time, and skin tissue defect liquefaction necrosis. Because the wound is difficult to heal and consumes a lot, it brings serious psychological pressure and economic burden to the patient. Wounds that have not healed for more than two weeks are called chronic skin ulcers, and wounds that have not healed for more than one month are called chronic refractory skin ulcers. In particular, foot ulcers caused by diabetes are common chronic complications of diabetes. Clinical manifestations include foot pain, foot ulcers, and foot gangrene, which can even lead to amputation. They are an important factor in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/5415A61P17/02A61P3/10
CPCA61K31/5415A61P3/10A61P17/02
Inventor 张媛媛孙晓东
Owner SICHUAN UNIV
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