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Production of D-pantoic acid and D-pantothenic acid

A pantothenate synthase and DNA sequence technology, applied in the field of plasmid DNA

Inactive Publication Date: 2003-10-15
BASF AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Methods (4), (8), and (9) also involve more troublesome processes because of the recovery, racemization, and reuse of the residual L-configuration

Method used

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  • Production of D-pantoic acid and D-pantothenic acid

Examples

Experimental program
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example

[0083] Hereinafter, the present invention is described in more detail by the following examples, which are only embodiments of the present invention, and they cannot limit the scope of the present invention in any way.

[0084] D-pantothenic acid can be quantified by high-performance liquid chromatography (column: Shimadzu SCR101H (7.9mm diameter × 30cm); microbial phase: 0.008N sulfuric acid; flow rate: 0.8ml / min; detector: differential refractometer) and / or microbial (indicator strain: Lactobacillus plantarum IFO3070; culture solution: commercially available pantothenic acid test culture solution (produced by DIFCO)). Quantitative determination of pantothenic acid and determination of optical purity were performed by liquid chromatography with high-performance ethyl acetate extracts after removal of cells from the culture broth by centrifugation (column: SUMICHIRAL OA-1200; microbial phase: n-hexyl alkane / 1,2-dichloroethane / ethanol=90 / 8 / 2; flow rate: 1.0 ml / min; detector: UV...

example 1

[0086] In the 200ml Erlenmeyer flask containing 20ml of the first sterilized culture fluid of the composition shown in table 3, inoculate the platinum circle Escherichia coli IFO3547 bacterial strain, FV5714 (IFO15368) bacterial strain, FV525 (IFO15369) bacterial strain, FV814 obtained from slant culture fluid (IFO15370) strain, or the FV521 strain, followed by rotational vibration cultivation at 30° C. for 20 hours at a rotation speed of 220 rpm. The 1ml portion of the first culture solution was transferred to a 200ml ribbed Erlenmeyer flask containing 20ml of the sterilized culture solution of the composition shown in Table 4, then cultivated at 38°C for 20 hours, and added 2.5ml, 54 % aqueous glucose solution, and cultured for another 24 hours. The amount and optical purity of pantothenic acid produced and the amount of accumulated pantothenic acid after the completion of the culture are given in Table 5.

[0087] table 3

[0088] Compositi...

example 2

[0103] In the 200ml Erlenmeyer flask containing the first sterilized culture fluid of the composition shown in Table 3, inoculate the Escherichia coli FV221 strain and the FV6051 bacterial strain obtained from the slant culture fluid, and then carry out 20 at 30°C with a rotating speed of 220rpm. Hours of rotary shaking culture. The 1ml portion of the first culture solution was transferred to a 200ml ribbed Erlenmeyer flask containing 40ml of the sterilized culture solution of the composition shown in Table 6, then cultivated at 38°C for 20 hours, and added 5ml, Immediately after the 54% aqueous glucose solution, the cells were incubated for another 24 hours. The amount and optical purity of pantothenic acid produced and the amount of accumulated pantothenic acid after the completion of the culture are given in Table 5.

[0104] Table 6 Culture solution ① (pH7.0) Culture solution ② (pH7.0) Component concentration Component concentration Corn extract 2.0% Cor...

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Abstract

The present invention relates to a method of producing D-pantothenic acid or a salt and a method of producing D-pantoic acid or a salt. D-pantothenic acid, D-pantoic acid or salts can be efficiently directly obtained microbiologically without using DL-pantoic acid, DL-pantolactone etc. as starting materials.

Description

technical field [0001] The present invention relates to a new production method and purification method of D-pantothenic acid and / or D-pantothenic acid (or its salt), a kind of microorganism capable of producing it, and a kind of Plasmid DNA of a gene region involved in the biosynthesis of its partial region. Pantothenic acid is a useful vitamin substance. D-pantothenic acid is an important intermediate substance used in the synthesis of pantothenic acid and CoA. Background technique [0002] Usually the production method of D-pantothenic acid (or its salt), namely a kind of important vitamin material comprises: (1) obtain D-pantothenic acid lactone and β-alanine (or its salt) in methanol chemical condensation method, (2) D-pantothenate hydrolysis method into D-pantothenic acid, D-configuration DL-pantothenate selective hydrolysis method into D-pantothenic acid, these two The methods all use microorganisms or an enzyme (unexamined published Japanese Patent Nos.228487 / 1989...

Claims

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Application Information

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IPC IPC(8): C12N1/21C12N15/52C12P7/42C12P13/02C12R1/19
CPCC12P13/02C12P7/42C12N15/52C12P7/26
Inventor 引地裕一守谷岳郎三木启司山口高正野上昱雄
Owner BASF AG