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Banana wilt prevention bio-organic fertilizer as well as preparation method and application thereof

A technology of bio-organic fertilizer and banana wilt, which is applied in the field of agricultural soil bio-fertilizer, can solve the problems of poor control effect and difficult survival, and achieve the effect of improving banana quality, outstanding effect and reducing risk

Inactive Publication Date: 2021-03-09
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have screened out some antagonistic fungi, bacteria, and actinomycetes that have inhibitory effects on banana wilt. A suitable carrier is added to the soil. Without a suitable carrier, it is difficult to survive and the control effect is not good.

Method used

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  • Banana wilt prevention bio-organic fertilizer as well as preparation method and application thereof
  • Banana wilt prevention bio-organic fertilizer as well as preparation method and application thereof
  • Banana wilt prevention bio-organic fertilizer as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of bacterial solution

[0019] Taking healthy banana orchard rhizosphere soil as the research object, Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX obtained after isolation and purification were screened and activated Finally, the activated colonies of ring 1 were picked respectively, inserted into 1000ml LB culture medium, placed in a constant temperature shaking incubator at 28°C, and cultured at 160rpm for 24h to form a bacterial solution.

[0020] Prepare several Erlenmeyer flasks with a capacity of 100mL, pour 20mL of PDA (pH value adjusted to 7) culture solution into the 100mL Erlenmeyer flasks, and carry out high-temperature sterilization after subpackaging, and set aside.

[0021] The three cultivated functional bacteria were inoculated into a 100mL Erlenmeyer flask containing 20mL of PDA culture solution at a ratio of 1:100, and then cultured at 30°C for 48h at a shaker speed of 120rpm to obtain a seed solution.

example 2

[0023] Control effect test of fungal strains on banana plant Fusarium wilt

[0024] 1.1 Materials and methods

[0025] 1.1.1 Test materials

[0026] Bananas to be tested: The variety of bananas to be tested is Saigon Banana, with 6 leaves.

[0027] Bacteria to be tested: FOC4 pathogenic bacteria, spore concentration 10 6 cfu / ml; antagonistic bacteria Bacillus subtilis KC, cell concentration 10 7 cfu / ml; antagonistic bacteria Paenibacillus polymyxa DN, cell concentration 10 7 cfu / ml; Mortierella longospora CB, cell concentration 10 7 cfu / ml; complex bacteria FH1 (Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX); mixed according to the number of colony-forming units at a ratio of 1:0.8:1 into), cell concentration 10 7 cfu / ml; complex bacteria FH2 (Bacillus subtilis, Paenibacillus polymyxa and Mortierellaelongata SX; mixed according to the number of colony forming units 1:1.2:1.4) , cell concentration 10 7 cfu / ml;

[0028] Composite bacteria CD (Bac...

example 3

[0047] 1. Preparation of bio-organic fertilizer 1

[0048] (1) Preparation of semi-finished organic fertilizer

[0049] Pig manure, cassava dregs, crushed banana stems and leaves, crushed banana peel, molasses fermentation powder and earthworm soil are the main raw materials, mixed according to the dry matter mass 50:25:25:25:3, stacked and fermented for 7 days to make organic Fat semi-finished products.

[0050] Earthworm soil is the soil obtained after 2 to 4 months of earthworm cultivation. The earthworm soil is obtained by adding 15 to 18% of crushed crustacean shells, 4.0 to 8.0% of potassium ore powder, and 1.5 to 4.5% of phosphate rock into the earthworm soil. Stone, 0.01-0.02% sodium borate.

[0051] (2) Fermentation culture

[0052] After adjusting the water content of the organic fertilizer semi-finished product to 40%, the seed liquids of Bacillus subtilis, Paenibacillus polymyxa and Mortierella elongata SX were divided into 1 community unit: 0.8:1 ratio is mixe...

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Abstract

The invention relates to the technical field of agricultural soil bio-fertilizers, and particularly discloses a banana wilt prevention bio-organic fertilizer as well as a preparation method and application thereof. The banana wilt prevention bio-organic fertilizer is prepared by the following steps: mixing pig manure, cassava residues, banana tree stems and leaves, banana peels, molasses ferment powder and earthworm soil according to the dry matter mass ratio of 50-55: 25-30: 25-30: 25-30: 3-5: 15-20, preparing an organic fertilizer semi-finished product after stack retting fermentation, inoculating compound bacteria consisting of bacillus subtilis, paenibacillus polymyxa and mortierella elongata, and carrying out secondary fermentation to prepare the banana wilt prevention bio-organic fertilizer. The high-quality organic fertilizer disclosed by the invention is found in banana planting application, and the fertilizer not only can improve soil for banana growth and increase the nutrient utilization rate, but also can effectively prevent and treat diseases and insect pests such as banana wilt and increase the banana yield.

Description

technical field [0001] The invention belongs to the technical field of agricultural soil biological fertilizers, in particular to a biological organic fertilizer for preventing banana wilt and its preparation method and application. Background technique [0002] Banana Fusarium wilt is a typical fungal soil-borne disease that destroys vascular bundles caused by the infection of Fusarium oxysporum Cuban-specialized. Among them, race 4 is the most serious damage. Bananas are found in almost all banana-producing areas in the world. Fusarium wilt occurs. At present, banana fusarium wilt has entered a stage of rapid expansion, which seriously threatens the healthy development of the banana industry. Finding effective control methods is an important issue of concern to major banana producing areas. Extensive use of chemicals pollutes the environment, destroys the ecological balance, and reduces the quality of bananas; the soil cleaning effect of banana orchards is not good; short...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/60C05F17/50C05F17/20
CPCC05B17/00C05F9/04C05F17/20C05F17/50C05G3/60C05D1/04C05D9/00C05D9/02C05F1/005C05F3/00C05F5/002C05F5/008C05F11/00C05F17/00Y02W30/40
Inventor 王瑾孟祥坤苏天明陈海宁何铁光高璐阳张强
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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