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Mirna receptivity analysis of the endometrium

An endometrial, receptive technique that is used for diagnostic and therapeutic purposes and can address issues such as low specificity

Pending Publication Date: 2021-03-09
英缇生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, compared with real-time quantitative polymerase chain reaction (qPCR) technology, microarray chip technology platform usually has lower specificity

Method used

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  • Mirna receptivity analysis of the endometrium
  • Mirna receptivity analysis of the endometrium
  • Mirna receptivity analysis of the endometrium

Examples

Experimental program
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example 1

[0105] Example 1: Materials and methods for generating miRNA expression profiles.

[0106] Endometrium specimens. Five days after progesterone administration in a hormone replacement therapy cycle (P+5) or seven days after the endogenous luteinizing hormone surge in a natural cycle (LH+7), endometrial suction curettes using the Pipelle (Cooper Surgical Company (Cooper Surgical, Inc.) collected endometrial samples from the female uterine cavity. The endometrial tissue was then stored in RNAlater.

[0107] RNA extraction and miRNA enrichment. Total RNA was isolated from endometrial tissue using the miRNeasy Micro Kit (QIAGEN) following the manufacturer's instructions. Briefly, five milligrams of endometrial tissue were disrupted with a motor and pestle and homogenized in liquid nitrogen. 700 microliters of QIAzol Lysis Reagent was added to the homogenized tissue, and the resulting sample was incubated at room temperature for five minutes to facilitate dissociation of the nuc...

example 2

[0110] Example 2: In silico miRNA analysis algorithms and their use.

[0111] like image 3 As shown in , an in silico miRNA analysis algorithm (MIRA) is constructed by performing one or more of the following steps: data normalization, data scaling, data transformation, predictive modeling, and cross-validation.

[0112] Data normalization. To make the distributions the same in statistical properties, normalize the data by percentile normalization. see image 3 Equation (A) in ; see also Bolstad et al., "A comparison of normalization methods for high density oligonucleotide array data based on variance and bias", Bioinformatics, 2003, 19(2): 185-193.

[0113] Data scaling. To ensure that the objective function is functioning properly, the numerical range of the data can be normalized so that the data have zero mean and unit variance. see image 3 Formula (B) in.

[0114] data conversion. For data reduction and feature extraction reasons, PCA compresses information from...

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Abstract

Provided are methods for determining an endometrial status using a sample, for example, an endometrial biopsy, from a woman, comprising: (a) performing an assay on the endometrial sample from the woman to determine a microRNA (miRNA) expression profile of the endometrial sample, wherein the miRNA expression profile comprises expression levels of a plurality of miRNAs, for example, 167 miRNAs having the sequences of SEQ ID NOs: 1-167, respectively; and (b) analyzing the miRNA expression profile to obtain a receptivity predictive score using, for example, a computer-based algorithm. Further provided are kits suitable for performing the methods, as well as uses of the kits for diagnostic and therapeutic purposes.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 869,574, filed July 2, 2019, which is incorporated herein by reference in its entirety. technical field [0003] The present disclosure relates to a method of determining endometrial receptivity in a woman using (a) a microribonucleic acid (miRNA) expression profile comprising expression levels of a plurality of miRNAs, for example 167 miRNAs; and ( b) a computer algorithm that classifies a woman's endometrial status based on the miRNA expression profile. Another aspect of the disclosure pertains to kits suitable for carrying out the methods, and the use of the kits for diagnostic and therapeutic purposes. In some embodiments, the methods and / or kits are used to classify a woman's responsiveness to a course of in vitro fertilization (IVF). Background technique [0004] Assisted reproductive technology (including IVF) has the potential t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/158C12Q2600/178C12Q1/686C12Q2600/112G06F17/18
Inventor 康诗婷陈伟铭
Owner 英缇生物科技股份有限公司
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