Bioactive peptide AAGGYDVEKNNSRIKLGLK as well as preparation method and application of bioactive peptide AAGGYDVEKNNSRIKLGLK

A bioactive peptide and active technology, applied in the field of protein, can solve problems such as the research of peptide fragments without protein, and achieve the effect of reducing the incidence rate, improving quality, and improving the ability to resist external pathogen infection

Active Publication Date: 2021-03-12
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the related functions of Histone H1.5 protein polypeptide fragments in the prior art

Method used

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  • Bioactive peptide AAGGYDVEKNNSRIKLGLK as well as preparation method and application of bioactive peptide AAGGYDVEKNNSRIKLGLK
  • Bioactive peptide AAGGYDVEKNNSRIKLGLK as well as preparation method and application of bioactive peptide AAGGYDVEKNNSRIKLGLK
  • Bioactive peptide AAGGYDVEKNNSRIKLGLK as well as preparation method and application of bioactive peptide AAGGYDVEKNNSRIKLGLK

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The artificial synthesis of embodiment 1 bioactive peptide AAGGYDVEKNNSRIKLGLK

[0030] 1. Synthesis of Bioactive Peptides

[0031] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak with 50ml of dichloromethane (DCM).

[0032] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) that is 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0033] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0034] 4. Take a small amount of resin and test it with ninhydrin (Ninhydrin Nine Wells) method (two drops each of test A and test B, react at 100°C for 1 min), if the resin has color, it means that the deprotection is successful.

[0035...

Embodiment 2

[0072] The immune activity experiment of embodiment 2 bioactive peptides

[0073] 1. The experiment of bioactive peptide AAGGYDVEKNNSRIKLGLK promoting macrophages to secrete cytokines (ELISA method)

[0074] 1. Experimental reagents and instruments:

[0075] Reagents: Experimental animal balb / c mice (male 6, 8 weeks old), Shanghai Slack Experimental Animal Co., Ltd.; mouse lymphocyte extract, Shanghai Suo Laibao Biotechnology Co., Ltd.; RPMI1640 medium, GIBCO Company; cattle Serum albumin (bovine serum albumin, BSA), Genebase Company; The mouse spleen lymphocyte-derived bioactive peptide AAGGYDVEKNNSRIKLGLK obtained in Example 1; ELISA cytokine rapid kit (TNF-α, IL-1β and IL-6) , Wuhan Boster Biological Engineering Co., Ltd.

[0076] Equipment: LRH, 250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL, 22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150 CO 2 Incubator Heraeus; Dragon WellscanMK3 microplate re...

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Abstract

The invention relates to the field of protein, in particular to a bioactive peptide AAGGYDVEKNNSRIKLGLK as well as a preparation method and application thereof, and the amino acid sequence of the bioactive peptide AAGGYDVEKNNSRIKLGLK is Ala-Ala-Gly-Gly-Tyr-Asp-Val-Glu-Lys-Asn-Asn-Ser-Arg-Ile-Lys-Leu-Gly-Leu-Lys. An in-vitro immunoregulation function experiment proves that the bioactive peptide AAGGYDVEKNNSRIKLGLK has a very good immunoregulation function. The bioactive peptide AAGGYDVEKNNSRIKLGLK disclosed by the invention can promote macrophages to secrete cytokines and phagocytize neutral red, improve the effect of the macrophages in a normal macrophage resting state, improve the capability of resisting external pathogen infection of organisms, reduce the morbidity of the organisms and improve the quality of life; the polypeptide has very important significance in developing food, health care products and medicines with immunoregulation functions.

Description

technical field [0001] The present invention relates to the protein field, in particular to a biologically active peptide AAGGYDVEKNNSRIKLGLK and its preparation method and application. Background technique [0002] In recent years, bioactive peptides have become a familiar term. Because of its many potential biological functions, it has attracted more and more attention and has become one of the hot spots of scientific research. At present, the beneficial effects of many bioactive peptides have also been well proven, such as anti-cancer, lowering blood pressure, antibacterial, lowering cholesterol, anti-diabetic and other properties. More than 3000 different bioactive peptides have been reported in the current most authoritative bioactive peptide database BIOPEP-UMW. [0003] At present, most studies on bioactive peptides focus on food-derived peptides, while there are few studies and reports on non-food-derived peptides. And it has been confirmed by research results tha...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12A61K8/64A61K38/17A61Q19/00A61P29/00A61P37/02A23L33/18
CPCC07K14/47A61K8/64A61Q19/00A61P29/00A61P37/02A23L33/18A61K38/00A23V2002/00A23V2200/324A23V2250/55
Inventor 张少辉王娟栾媛媛占文静张伯宇郭婷婷李政谢雨佳
Owner SHANGHAI JIAO TONG UNIV
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