Serum amyloid protein A immunochromatographic test strip, kit and preparation method thereof

Abstract

The invention discloses a serum amyloid protein A immunochromatography test strip, which comprises a sample pad, a combination pad, a reaction film and a water absorption pad, the reaction film is provided with a detection line and a quality control line which are mutually spaced, and the combination pad is coated with a first serum amyloid protein A monoclonal antibody and a quality control antibody which are respectively labeled by fluorescent microspheres. The detection line is coated with a second serum amyloid protein A monoclonal antibody, the quality control line is coated with an antigen capable of being specifically combined with the quality control antibody, and the detection line and the quality control line are calculated according to the surface area of the sample pad. The sample pad is coated with an antibody of a non-detection sample species with a concentration of 1 [mu]g / cm2-16 [mu]g / cm2, lectin with a concentration of 0.2 [mu]g / cm2-16 [mu]g / cm2, and a blocking agent for an heterophilic antibody of a detection sample with a concentration of 1 [mu]g / cm2-16 [mu]g / cm2. The invention also discloses a preparation method of the serum amyloid protein A immunochromatographic test strip. The invention also discloses a kit.

Description

technical field [0001] The invention relates to a detection test paper, in particular to a serum amyloid A immunochromatographic test paper strip and a preparation method thereof. Background technique [0002] Serum amyloid A (SAA) is an acute phase protein secreted by the liver and bound to high-density lipoprotein (HDL) in plasma. When the body is in an inflammatory state, the concentration of SAA will increase rapidly within a few hours; at the same time, the half-life of SAA is very short, and the concentration of SAA will decrease rapidly as the source of inflammation is eliminated. In veterinary medicine, SAA is a sensitive marker of inflammation and tissue damage. Existing SAA detection methods are mainly enzyme-linked immunoassay, chemiluminescence, colloidal gold immunochromatography and so on. At present, the detection methods of TSHD mainly include traditional radioimmunoassay, enzyme-linked immunosorbent assay, and chemiluminescence. Among them, radioimmunoass...

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Problems solved by technology

[0004] Based on this, it is necessary to provide a serum amyloid A immunochromatographic test strip and its preparation method for the problem that the traditional SAA test card cannot meet the emergency requirements, which can not only obtain the result quickly, but also have a small amount of sampling and can Meet the detection requirements of animals, especially small animals with impaired physical function

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