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Application of lactobacillus reuteri bacterium agent containing reuterin to preparation of preparation for promoting glycolipid metabolism of pelteobagrus fulvidraco

A technology of Lactobacillus reuteri and Reuterin, which is applied in the field of aquaculture, can solve the problems affecting the meat quality and commercial value of yellow catfish, disorder of glucose and lipid metabolism in yellow catfish, accumulation of abdominal fat, etc., and achieves improvement. Effects of intestinal flora distribution, inhibition of harmful bacteria colonization, and regulation of glucose and lipid metabolism

Pending Publication Date: 2021-03-16
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Yellow catfish is an important freshwater aquaculture species in my country, with delicious meat and high nutritional value. At present, it is mainly raised intensively. Due to reasons such as unreasonable feed formula (fat and sugar ratio), the glucose and lipid metabolism of yellow catfish Disturbance, fat accumulation in the abdominal cavity, and enlarged liver have seriously affected the meat quality and commodity value of the yellow catfish

Method used

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  • Application of lactobacillus reuteri bacterium agent containing reuterin to preparation of preparation for promoting glycolipid metabolism of pelteobagrus fulvidraco
  • Application of lactobacillus reuteri bacterium agent containing reuterin to preparation of preparation for promoting glycolipid metabolism of pelteobagrus fulvidraco
  • Application of lactobacillus reuteri bacterium agent containing reuterin to preparation of preparation for promoting glycolipid metabolism of pelteobagrus fulvidraco

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1 strain activation

[0028] First prepare the strains, aseptically open the freeze-dried strain Lactobacillus reuteri (from the Guangdong Microbial Culture Collection Center, strain number: GIM1.614), inoculate it on the slant of the MRS agar test tube, and incubate at 37±1°C 18 hours; then transferred to MRS liquid medium, 37 ± 1 ° C static culture for 18 hours, the formation of a seed solution, the concentration of 8.3 × 10 8 cfu / mL.

[0029] 2 culture and fermentation

[0030] The primary seed liquid is transferred to the fermentation medium producing glycerol dehydratase with a volume ratio of 3% inoculum for fermentation. The liquid volume in the fermentation tank is 50%, the fermentation temperature is 28 ° C, micro-aerobic cultivation, and the rotating speed is 80 rpm. 6mol / LNaOH to adjust the pH, control the pH of the fermentation broth to 5.5, the fermentation time is 16h, the number of viable Lactobacillus reuteri in the fermentation broth is 9.6×10 8 cfu / ...

Embodiment 2

[0045] 1 strain activation

[0046] First prepare the strains, aseptically open the freeze-dried strain Lactobacillus reuteri (from the Guangdong Microbial Culture Collection Center, strain number: GIM1.614), inoculate it on the slant of the MRS agar test tube, and incubate at 37±1°C 23 hours; then transferred to MRS liquid medium, 37 ± 1 ° C static culture for 24 hours, the formation of seed liquid, the concentration of 1.1 × 10 9 cfu / mL.

[0047] 2 culture and fermentation

[0048] 2.1 Preparation of primary seed solution

[0049] Transfer the seed liquid to a fermenter containing a primary fermentation medium with an inoculum volume of 4% by volume. The liquid volume of the fermenter is 70%, the fermentation temperature is 37±1°C, and the culture is airtight and static, and the fermentation time is 20h. Make a first-grade seed solution, its concentration is 1.2×10 9 cfu / mL.

[0050] 2.2 Culture and fermentation

[0051] The primary seed liquid is transferred to the fe...

Embodiment 3

[0063] 1 strain activation

[0064] First prepare the strains, aseptically open the freeze-dried strain Lactobacillus reuteri (from the Guangdong Microbial Culture Collection Center, strain number: GIM1.614), inoculate it on the slant of the MRS agar test tube, and incubate at 37±1°C 28 hours; then transferred to MRS liquid medium, 37 ± 1 ° C static culture for 30 hours, the formation of seed liquid, the concentration of 1.7 × 10 9 cfu / mL.

[0065] 2 culture and fermentation

[0066] 2.1 Preparation of primary seed solution

[0067] Transfer the seed liquid to a fermenter containing a primary fermentation medium with an inoculation amount of 6% by volume. The liquid volume of the fermenter is 80%, the fermentation temperature is 37±1°C, and the culture is airtight and static, and the fermentation time is 24h. Make a first-class seed solution, its concentration is 1.3×10 9 cfu / mL.

[0068] 2.2 Culture and fermentation

[0069] The primary seed liquid is transferred to the f...

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Abstract

The invention discloses a lactobacillus reuteri bacterium agent for improving utilization of high-sugar feed of pelteobagrus fulvidraco and a preparation method thereof. The lactobacillus reuteri bacterium agent with high content of reuterin, which is disclosed by the invention, contains high content of reuterin; and compared to a conventional lactobacillus reuteri bacterium agent (which does notcontain high content of reuterin), the combination of high content of reuterin and lactobacillus reuteri can further improve the growth performance and the feed utilization rate of the pelteobagrus fulvidraco, obviously improve the glycolipid metabolism capability and the immunity, and reduce the occurrence of diseases. Therefore, according to the invention, by exogenously adding the lactobacillusreuteri bacterium agent with high content of reuterin, which is disclosed by the invention, into a high-sugar feed, feed utilization can be improved, the glycolipid metabolism of the pelteobagrus fulvidraco is effectively promoted, abdominal cavity lipopexia is reduced, occurrence of the fatty liver is reduced, non-specific immunity of the pelteobagrus fulvidraco is improved, and occurrence of diseases is reduced.

Description

Technical field: [0001] The invention belongs to the field of aquaculture, and in particular relates to the application of a Lactobacillus reuteri bacterial agent containing reuterin in the preparation of a preparation for promoting glucose and lipid metabolism of yellow catfish. Background technique: [0002] Sugar is the cheapest energy substance in the feed. Adding it in an appropriate amount can save protein, promote fish growth, reduce feed cost, and reduce environmental pollution caused by nitrogen emissions. However, fish have a relatively low utilization rate of sugar. Excessive sugar levels in feed will have a great impact on the health of fish, such as growth inhibition, decreased feed utilization, liver enlargement and lesions, and persistent high blood sugar. The wide application of sugar in compound feed. Gut microbes and the body form a host-microbial metabolic axis, which plays an important role in the metabolism of nutrients and immune homeostasis in animals...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/18A23K10/37A23K10/12A61K35/747A61P3/00A61P1/00A61P37/04
CPCA23K50/80A23K10/18A23K10/37A23K10/12A61K35/747A61P3/00A61P1/00A61P37/04Y02P60/87
Inventor 张丽许国焕马先义成艳波李志朋
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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