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In-vitro hybridization method for gerbera jamesonii

A technology of isolated and hybrid seeds, which is applied in the direction of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of reducing breeding efficiency, hybridization failure, and limited opportunities for hybrid breeding, etc., and achieves easy and fast control of temperature and humidity. The process of breeding, the efficient effect of cross breeding

Pending Publication Date: 2021-03-26
三明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After cross-pollination of gerbera, if the air humidity is high, the inflorescences are prone to mold and rot, resulting in failure of hybridization; high temperature and dry climate conditions are also prone to low or even no fruiting of hybridization
In most parts of China, it is rainy and humid in spring, high temperature and drought in summer, and low temperature in winter. The humidity in the greenhouse is high due to the need to cover the film for heat preservation. The opportunities for hybrid breeding are very limited, which seriously reduces the efficiency of breeding.

Method used

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  • In-vitro hybridization method for gerbera jamesonii
  • In-vitro hybridization method for gerbera jamesonii
  • In-vitro hybridization method for gerbera jamesonii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A hybridization method of gerbera, comprising the following steps:

[0027] (1) Parental material selection: From March to May, choose a flower branch with a regular flower pattern, no pests and diseases, and 2-3 rounds of anthers in the inflorescence to start to loose powder as the male parent of the hybrid. Choose the long 60-70cm of pedicel, the thick 0.6-0.8cm of pedicel, the ligulate flower of outer wheel is flat, the first round anther does not take off powder yet, and flower pattern is regular, and the flower branch without damage by diseases and insect pests is used as female parent material. .

[0028] (2) Collect pollen: When the flower branch of the male parent looses pollen, break off the flower branch, shake the pollen into a petri dish with a diameter of 7 cm, cover the dish, and bring it back indoors for pollination.

[0029] (3) Treatment of female parent material: remove the female parent flower branches, bring them back indoors, cut off the outer ligu...

Embodiment 2

[0034] (1) Parental material selection: From July to September, a flower branch with a regular flower pattern, no pests and diseases, and 3-4 rounds of anthers in the inflorescence starting to loose powder is selected as the male parent of the hybrid. Select the long 50-60cm of pedicel, the thick 0.7-0.9cm of pedicel, the ligulate flower of outer wheel is flat, the first round of anthers does not take powder yet, the pattern of flowers is regular, and the flower branch without damage by diseases and insect pests is used as female parent material.

[0035] (2) Collect pollen: When the flower branch of the male parent looses pollen, break off the flower branch, shake the pollen into a 9cm-diameter petri dish, cover the dish, and bring it back indoors for pollination.

[0036] (3) Treatment of female parent material: remove the female parent flower branch, bring it back indoors, cut off the outer ligulate petals with scissors, leaving a margin of 0.3-0.8cm to avoid damage to the s...

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Abstract

The invention discloses an in-vitro hybridization method for gerbera jamesonii and belongs to the technical field of gerbera jamesonii crossbreeding. Male parent pollens are collected, a flower cutting in-vitro vase pollination of gerbera jamesonii is carried out, and hybrid seeds are obtained. The method provides a new way for breeding, overcomes adverse factors such as high temperature, high humidity, diseases and the like which are easy to occur in a hybridization process of gerbera jamesonii, and remarkably improves a success rate of gerbera jamesonii hybridization.

Description

technical field [0001] The invention belongs to the technical field of gerbera hybrid breeding, and in particular relates to a method for obtaining hybrid seeds by in vitro pollination of cut gerbera flowers. Background technique [0002] Gerbera is a perennial herbaceous flower of the genus Gerbera in the Compositae family. It has large flowers, rich colors, long shelf life, annual flowers, long-distance transportation resistance, and strong decoration. It is an ideal material for gift bouquets, flower baskets and artistic flower arrangements. It is one of the top five fresh cut flowers in the world and has a broad market prospect. [0003] Gerbera is native to South Africa, and China is not the origin of gerbera, so breeding mainly relies on foreign mature varieties for selection. With the rapid development of modern biotechnology, a large number of advanced breeding techniques have been developed and applied, including mutation breeding, ploidy breeding, molecular marker...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01N3/02
CPCA01H1/02A01N3/02
Inventor 曹奕鸯夏朝水陈昌铭陈玮婷林辉锋周辉明莫智龙林发壮姚凤琴钟琳珊
Owner 三明市农业科学研究院
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