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Method for determining bioavailability of arsenic in cordyceps sinensis based on in-vitro digestion/Caco2 cell model

A technology of cell model and Cordyceps sinensis, which is applied in the preparation of test samples, measuring devices, and material analysis through electromagnetic means, can solve the problems of different bioavailability detection methods, and achieve the effect of saving traditional Chinese medicine resources and promoting development

Pending Publication Date: 2021-03-26
NAT INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

However, the heavy metal pollution in the site soil is quite different from the existing form of heavy metals in traditional Chinese medicine, the types of heavy metals, and the limit standards of heavy metals. Moreover, the oral administration of traditional Chinese medicine and the ingestion of heavy metals in the site soil through breathing and other methods have caused bioavailability. different detection methods
There is currently no method for evaluating the bioavailability of heavy metals in traditional Chinese medicines using an in vitro digestion / Caco2 cell model

Method used

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  • Method for determining bioavailability of arsenic in cordyceps sinensis based on in-vitro digestion/Caco2 cell model
  • Method for determining bioavailability of arsenic in cordyceps sinensis based on in-vitro digestion/Caco2 cell model
  • Method for determining bioavailability of arsenic in cordyceps sinensis based on in-vitro digestion/Caco2 cell model

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Embodiment 1

[0042] Cordyceps sinensis samples (purchased from the Tibet Lhasa Medicine Market) Crushing, 50 mesh sieve, precisely 0.5g Cordyceps sinensis powder, add 50 ml to simulate gastric fluid (from 1.25 g of gastric protease, 0.5 g sodium citrate, 0.5 g sodium maline, 420μL lactic acid It is formulated with 500 μl of acetic acid, and the dissolution is reached to 1 L, and the pH is adjusted to 1.5 by HCl). The argon gas is 1 to 2 minutes, and an anaerobic environment in the gastrointestinal tract is simulated. Evergings 1 h in a constant temperature water bath at 37 ° C (100R · min -1 ), Centrifugation 5 min (4000R · min -1 ), To obtain residue and supernatant layers. The precision amount was taken 25 ml, and the electric hot plate was concentrated to about 3 mL, and 5 ml of nitric acid was added, and the microwave digestometer was decomposed. After the elimination is cooled, the ultrapure water is set to 50 ml of volumetric flask to obtain "extraction gastric liquid" to be tested. The ...

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Abstract

The invention provides a method for determining bioavailability of arsenic in cordyceps sinensis based on an in-vitro digestion / Caco2 cell model, and belongs to the technical field of heavy metal biological availability detection. According to the method, a Caco-2 intestinal absorption model is established by adopting transwell culture, the in-vitro digestion / Caco-2 cell model is established by adopting a two-step digestion method for simulating gastric and intestinal digestion in vitro, and the biological availability of arsenic in cordyceps sinensis is determined by adopting an ICP-MS method, so a basis is provided for scientifically and objectively evaluating health risks of heavy metals in traditional Chinese medicines and modifying limit standards. Experiments prove that after Caco-2cells in cordyceps sinensis are transferred, the biological availability of arsenic is 1.5%-13.0%. According to the method, the development of the traditional Chinese medicine industry is promoted, resources are saved; meanwhile, a new thought is provided for objectively and scientifically evaluating the health risk of the heavy metal in the traditional Chinese medicine to the human body, and a basis is provided for formulating a scientific and reasonable heavy metal limit standard.

Description

Technical field [0001] The present invention belongs to the field of heavy metal biological biological detection, and in particular, the present invention relates to a biological active method for determining the arsenic in Cordyceps on the determination of arsenpicine in the in vitro digestion / CaCO2 cell model. Background technique [0002] The toxicity evaluation of the heavy metal in Chinese medicine is not only related to the absolute amount of its intake, but also is closely related to the content actually absorbed. If the content of the heavy metal in the Chinese medicine is used to assess its risk, it may overestimate the harm to the human body. Bioaccessibility of heavy metals, is the ratio of heavy metals that can be dissolved in the gastrointestinal environment, which is an indicator of its maximum oral bioavailability (BioavaiLability). Animal test is the most effective means to evaluate heavy metal bio, but animal experiments are complicated and cumbersome, and the ...

Claims

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Application Information

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IPC IPC(8): G01N27/626G01N1/28G01N1/34
CPCG01N27/626G01N1/28G01N1/34
Inventor 左甜甜罗飞亚马双成金红宇孙磊邢书霞李波高飞康帅
Owner NAT INST FOR FOOD & DRUG CONTROL
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