Space full-automatic multifunctional bioreactor based on microsatellite
A bioreactor and multi-functional technology, applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, enzymology/microbiology device, etc., can solve the problem that microsatellites cannot carry human and cell biology The reactor cannot meet the requirements of microsatellites and other issues
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Embodiment 1
[0047] This embodiment provides a space fully automatic multifunctional tumor stem cell bioreactor based on microsatellites, such as figure 1 As shown, the bioreactor integrates the first liquid culture unit 100 for tumor stem cell renewal and proliferation, the second liquid culture unit 200 for stem cell drug resistance research, and the first hydrogel three-dimensional culture for stem cell migration research Cell multifunctional culture module of unit 300 and the second hydrogel three-dimensional culture unit 400 for stem cell invasion research.
[0048] Preferably, the first liquid culture unit 100 is composed of a plurality of first cell suspension culture units 108 preferably cascaded or connected in series. The second liquid culture unit 200 is composed of a plurality of second cell suspension culture units 208 cascaded or connected in series. Each of the first cell suspension culture unit 108 and the second cell suspension culture unit 208 is provided with a liquid i...
Embodiment 2
[0060] This embodiment discloses an experimental method for tumor stem cells based on microsatellites, which integrates the first liquid culture unit 100 for tumor stem cell renewal and proliferation, the second liquid culture unit 200 for stem cell drug resistance research, and the second liquid culture unit 200 for stem cell migration research. The cell multifunctional culture module of the first hydrogel three-dimensional culture unit 300 and the second hydrogel three-dimensional culture unit 400 used for stem cell invasion and vascularization research simultaneously conduct self-renewal and proliferation, drug resistance, migration and invasion experiments of stem cells .
[0061] Preferably, the first liquid culture unit 100 and the second liquid culture unit 200 are inoculated with cells on the ground, and the cells are input into the culture units until they are full. After the satellite enters orbit, self-renewal, proliferation and drug resistance experiments are carrie...
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