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Multifunctional organ chip based on microfluidic technology, preparation method and application thereof

A microfluidic technology, organ chip technology, applied in stress-stimulated microbial growth methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as difficult to correctly reflect human cornea

Inactive Publication Date: 2021-03-30
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The present invention proposes a method for preparing a multi-functional organ chip based on microfluidic technology and its application, which can not only stably cultivate human corneal cells in the culture area to form three-dimensional corneal tissue, but also monitor the integrity of the established corneal barrier. Detection, to solve the problem that it is difficult to correctly reflect the human cornea in two-dimensional culture in vitro and animal experiments in vivo, and meet the needs of scientific research and clinical applications for in vitro bionic research models

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  • Multifunctional organ chip based on microfluidic technology, preparation method and application thereof
  • Multifunctional organ chip based on microfluidic technology, preparation method and application thereof
  • Multifunctional organ chip based on microfluidic technology, preparation method and application thereof

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Embodiment 1

[0092] A multifunctional organ chip based on microfluidic technology, see Figure 1-Figure 4 as well as Figure 9 . see figure 1 , the chip comprises two structural layers, the upper layer 1 is the upper layer 1 polydimethylsiloxane (Polydimethylsiloxane, PDMS) with the first microchannel 4, the first cell culture area 5 and the first test area 9, and the lower layer porous membrane 8 is a polycarbonate membrane with a microporous structure, the lower layer 2 is a lower layer 2PDMS with a second microchannel 6, a second cell culture area and a second test area 10, and the substrate 3 is a glass-based substrate.

[0093] see figure 2 , the upper left corner and the lower right corner of the chip of the present invention are the inlet and the outlet of the first microchannel 4 respectively, the lower left corner and the upper right corner of the chip are respectively the inlet and the outlet of the second microchannel 6; the three-dimensional cell culture area in the middle ...

Embodiment 2

[0099]A preparation method of a multifunctional organ chip based on microfluidic technology: taking two structural layers as an example:

[0100] The chip manufacturing process includes three parts: upper layer 1PDMS manufacturing, lower layer 2PDMS manufacturing and chip bonding, such as Figure 5 shown. The manufacturing steps of the upper layer 1 PDMS include: making the upper layer 1 microchannel male mold, pouring the upper layer 1 PDMS, turning over the upper layer 1 PDMS, and punching the upper layer 1 PDMS, see Figure 5 Neutron Figure S1. The manufacturing steps of the lower layer 2PDMS include: making the lower layer 2 microchannel male mold, lower layer 2PDMS pouring, lower layer 2PDMS turning over, and lower layer 2PDMS drilling, see Figure 5 Neutron Figure S2. Then, first bond the lower layer 2PDMS to the glass substrate, then seal the second cell culture area 7 of the lower layer 2PDMS with a polycarbonate film, and finally align and bond the upper layer 1PDM...

Embodiment 3

[0125] The multifunctional organ chip based on microfluidic technology of the present invention can be used to construct human organs. The following uses the human cornea as an example to illustrate:

[0126] A method for simulating the physiological microenvironment of the human eye with a multifunctional organ chip based on microfluidic technology, comprising the following steps:

[0127] The microenvironment of the human cornea can be simulated by using the above-mentioned organ chip. This part will introduce the construction method and operation steps of the eye organ chip in detail, mainly including chip pretreatment, three-dimensional cell culture, sample collection, analysis and characterization. The flow chart is as follows: Figure 7 shown.

[0128] Sub-step C1, chip pretreatment:

[0129] The microfluidic chip is sterilized by ultraviolet irradiation for more than or equal to 30 minutes in a clean bench, and the chip is modified by adding type 1 collagen, placed at ...

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Abstract

The invention belongs to the field of microfluidic technology and biotechnology, and relates to a multifunctional organ chip based on a microfluidic technology, and a preparation method and an application thereof. The multifunctional organ chip based on the microfluidic technology comprises a substrate, the substrate at least comprises two structural layers, each structural layer is provided witha micro-channel and a cell culture area communicated with the micro-channel, the cell culture areas of every two adjacent structural layers are communicated, and a porous membrane is arranged at the communication position. The invention provides the multifunctional organ chip based on the microfluidic technology, which not only can stably culture human corneal cells in a culture area to form three-dimensional corneal tissues, but also can detect the integrity of an established corneal barrier, and solves the problem that in-vitro two-dimensional culture and in-vivo animal experiments are difficult to correctly reflect human corneas, and the requirements of scientific research and clinical application on an in-vitro bionic research model are met.

Description

technical field [0001] The invention belongs to the field of microfluidic technology and biotechnology, and relates to a multifunctional organ chip based on microfluidic technology, a preparation method and an application thereof. Background technique [0002] The eye is the human visual organ and one of the most important organs in the human senses. It is composed of the eyeball and its auxiliary structures. About 80% of the information in the brain is obtained through the eyes. With the improvement of economic level, the change of people's lifestyle and the change of population structure in our country, the disease spectrum of blinding eye diseases has undergone major changes. The main blinding eye diseases have changed from trachoma infectious eye diseases in the past to cataracts, Corneal diseases, retinal diseases, refractive errors, glaucoma, and metabolic and age-related non-infectious eye diseases dominated by amblyopia are the challenges faced by my country's eye hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06C12M3/00C12M1/34C12N5/071B01L3/00
CPCB01L3/5027B01L2200/10C12M21/08C12M23/16C12M23/20C12M23/48C12M29/04C12M35/08C12M41/46C12N5/0621C12N2513/00
Inventor 杨慧于子桐郝锐张翊
Owner SHENZHEN INST OF ADVANCED TECH
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