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Pumpkin breeding method of male sterile dual-purpose line GMS-Ma1802

A gms-ma1802, male sterility technology, applied in the agricultural field, can solve the problems of high cost, increased labor cost, incomplete emasculation, etc., and achieve the effects of stable offspring traits, quality assurance, and simple reproduction.

Inactive Publication Date: 2021-04-02
HUNAN UNIV OF HUMANITIES SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For quite a long period of time, because pumpkin is a large-flowered and often cross-pollinated crop, traditional emasculation and isolation breeding and hybrid seed production have no obvious impact on industrial development.
However, with the increase in the scale of the pumpkin industry recently, the production and promotion area of ​​hybrid varieties has increased rapidly. The workload and cost of removing male flowers in hybrid seed production have been large, and the labor cost has increased rapidly. In some areas, the labor cost has accounted for 30% of the production cost of pumpkin hybrid seeds. %above
Especially in the middle and later stages of flowering, when the stems and leaves are sealed and emasculated, it is difficult to observe, and emasculation is not complete, so it is difficult to ensure the purity of hybrids, thereby affecting the yield and quality of pumpkins

Method used

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  • Pumpkin breeding method of male sterile dual-purpose line GMS-Ma1802
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  • Pumpkin breeding method of male sterile dual-purpose line GMS-Ma1802

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1, the acquisition of the male sterile dual-purpose line GMS-Ma1802,

[0028] In 2017, a sterile mutant was found in the Indian squash high-generation inbred line material 10-32 that was mutagenized with 0.2 mol L-1 ethyl methanesulfonate (EMS) for 20 minutes. After 4 consecutive generations Intra-hybridization, after the male fertile plant and the sterile plant in the line are crossed, the reproduced offspring are all stable as 50% male fertile and 50% male sterile, except for the difference in male flower fertility between the male sterile plant and the male fertile plant In addition, the traits of the rest of the plants and fruits are consistent, and the genetic traits are gradually stable, which is a stable male sterile dual-purpose line.

[0029] Indian pumpkin (Cucurbita maxima) male sterile dual-purpose line GMS-Ma1802 has been preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short) on October 12,...

Embodiment 2

[0035] Example 2. Application of the male sterile line GMS-Ma1802 (using 18-04-3 and 18-37-2 as male parents)

[0036] 1. The male sterile line GMS-Ma1802 was used for pumpkin breeding.

[0037]1. In 2018, the pollen of 18-04-3 and 18-37-2 were used to pollinate the female flowers of the male sterile dual-purpose line GMS-Ma1802, and the greenhouse was managed normally. Two kinds of F1 generation seeds were obtained in July 2018. At the same time, the male and female flowers of the male fertile plant of GMS-Ma1802 were pollinated to the male and female flowers of the fertile plant, and the greenhouse was managed normally, and the F2 generation seeds of GMS-Ma1802 were obtained in July 2018

[0038] 2. In the autumn of 2018, the F2 generation seeds obtained in step 1 were planted in Hunan, and the two types of F1 seeds obtained in step 1 were planted, and treated in parallel, and the growth of the plants was continuously observed.

[0039] 2. Character analysis

[0040] 1. In...

Embodiment 3

[0042] Example 3. Application of the male sterile dual-purpose line GMS-Ma1802 (using 18-04-3 and 18-37-2 as male parents)

[0043] 1. Hybridization first and then backcrossing (using 18-04-3 and 18-37-2 as male parents)

[0044] 1. In the spring of 2018, 18-04-3 and 18-37-2 were used as male parents to pollinate the female flowers on the male sterile plants of the male sterile dual-purpose line GMS-Ma1802 during the flowering period, and the two species were harvested under normal management. Seed F1.

[0045] 2. In the autumn of 2018, the two F1 species obtained in step 1 were planted in Hunan, and the male sterile line GMS-Ma1802 was planted. When flowering, the male sterile line GMS was pollinated by the male flowers of the two F1 species. -Female flowers on male sterile plants of Ma1802, managed normally and harvested 2 backcross BC1 seeds respectively.

[0046] 3. In the spring of 2019, the two F1 species obtained in step 1 were planted in Hunan, the male sterile line ...

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PUM

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Abstract

The invention provides a pumpkin breeding method of a male sterile dual-purpose line GMS-Ma1802. The male sterile dual-purpose line GMS-Ma1802 serves as a female parent and is hybridized with other pumpkins serving as male parents to obtain an F1 generation, and male sterile plants in the F1 generation are target plants for breeding. According to the pumpkin breeding method, application of male sterility to pumpkin breeding is realized, and the labor cost for carrying out female parent male flower isolation bagging or castration in pumpkin hybrid seed production is saved. The effects of promoting pumpkin hybrid variety popularization and increasing farmer income are increasingly shown, and male sterile material breeding and production application prospects are very wide.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a pumpkin breeding method based on the male sterile line GMS-Ma1802. Background technique [0002] Plant male sterility has been used as an important tool for hybrid seed production and dominant utilization of various crops, especially the use of heterosis in self-pollinated crops and often cross-pollinated crops. Male sterility has been used as an important approach, such as Rice, pepper, cotton, cabbage, corn, cabbage, etc. Cucurbitaceae crops are mostly heterogeneous crops. At present, there are research reports on Cucurbitaceae crops such as watermelon and melon, but there are few applications of male sterility, and even fewer application research reports. [0003] The main factor affecting the male sterility of Cucurbitaceae is that it is difficult to obtain materials with stable male sterility, and the male sterility that can be applied is rare. For quite a long tim...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/00
CPCA01H1/02A01H1/00
Inventor 刘泽发姜艳芳谢波朱明涛龚意辉余俊曾永贤
Owner HUNAN UNIV OF HUMANITIES SCI & TECH
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