Pumpkin breeding method of male sterile dual-purpose line GMS-Ma1802
A gms-ma1802, male sterility technology, applied in the agricultural field, can solve the problems of high cost, increased labor cost, incomplete emasculation, etc., and achieve the effects of stable offspring traits, quality assurance, and simple reproduction.
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Embodiment 1
[0027] Example 1, the acquisition of the male sterile dual-purpose line GMS-Ma1802,
[0028] In 2017, a sterile mutant was found in the Indian squash high-generation inbred line material 10-32 that was mutagenized with 0.2 mol L-1 ethyl methanesulfonate (EMS) for 20 minutes. After 4 consecutive generations Intra-hybridization, after the male fertile plant and the sterile plant in the line are crossed, the reproduced offspring are all stable as 50% male fertile and 50% male sterile, except for the difference in male flower fertility between the male sterile plant and the male fertile plant In addition, the traits of the rest of the plants and fruits are consistent, and the genetic traits are gradually stable, which is a stable male sterile dual-purpose line.
[0029] Indian pumpkin (Cucurbita maxima) male sterile dual-purpose line GMS-Ma1802 has been preserved in the General Microbiology Center of China Committee for Microbial Culture Collection (CGMCC for short) on October 12,...
Embodiment 2
[0035] Example 2. Application of the male sterile line GMS-Ma1802 (using 18-04-3 and 18-37-2 as male parents)
[0036] 1. The male sterile line GMS-Ma1802 was used for pumpkin breeding.
[0037]1. In 2018, the pollen of 18-04-3 and 18-37-2 were used to pollinate the female flowers of the male sterile dual-purpose line GMS-Ma1802, and the greenhouse was managed normally. Two kinds of F1 generation seeds were obtained in July 2018. At the same time, the male and female flowers of the male fertile plant of GMS-Ma1802 were pollinated to the male and female flowers of the fertile plant, and the greenhouse was managed normally, and the F2 generation seeds of GMS-Ma1802 were obtained in July 2018
[0038] 2. In the autumn of 2018, the F2 generation seeds obtained in step 1 were planted in Hunan, and the two types of F1 seeds obtained in step 1 were planted, and treated in parallel, and the growth of the plants was continuously observed.
[0039] 2. Character analysis
[0040] 1. In...
Embodiment 3
[0042] Example 3. Application of the male sterile dual-purpose line GMS-Ma1802 (using 18-04-3 and 18-37-2 as male parents)
[0043] 1. Hybridization first and then backcrossing (using 18-04-3 and 18-37-2 as male parents)
[0044] 1. In the spring of 2018, 18-04-3 and 18-37-2 were used as male parents to pollinate the female flowers on the male sterile plants of the male sterile dual-purpose line GMS-Ma1802 during the flowering period, and the two species were harvested under normal management. Seed F1.
[0045] 2. In the autumn of 2018, the two F1 species obtained in step 1 were planted in Hunan, and the male sterile line GMS-Ma1802 was planted. When flowering, the male sterile line GMS was pollinated by the male flowers of the two F1 species. -Female flowers on male sterile plants of Ma1802, managed normally and harvested 2 backcross BC1 seeds respectively.
[0046] 3. In the spring of 2019, the two F1 species obtained in step 1 were planted in Hunan, the male sterile line ...
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