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Medical application of oligopeptide and copper ion chelate thereof

A copper ion and chelate technology, applied in the field of treatment of smoking-related respiratory diseases, can solve the problem of no research examining the effect of GHK-Cu, so as to inhibit smoking-related diseases, inhibit the occurrence and development, and inhibit airway remodeling. Effect

Pending Publication Date: 2021-04-02
CHINA JAPAN FRIENDSHIP HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, no studies have examined the effect of GHK-Cu on smoking-related respiratory diseases other than the present invention

Method used

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  • Medical application of oligopeptide and copper ion chelate thereof
  • Medical application of oligopeptide and copper ion chelate thereof
  • Medical application of oligopeptide and copper ion chelate thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Establishment of mouse emphysema model, experimental grouping and administration method.

[0022]Firstly, the cigarette smoke extract (cigarette smoking extract, CSE) was prepared, and the smoke produced by burning 3 cigarettes with filter tips (Marlboro, Philip Morris, nicotine 0.8mg, tar 10mg, carbon monoxide 10mg) was dissolved in 15ml In the phosphate buffered saline (phosphate buffered saline, PBS) solution, the obtained solution was defined as 100% CSE, and the solution was filtered twice with a 0.22 μm filter. The obtained solution should be used within 30 minutes after preparation. The C57BL / 6 mice were randomly divided into 5 groups: control group, CS-induced mouse emphysema model group (CS group), CS+0.2μg / g GHK-Cu group, CS+2μg / g GHK-Cu group, CS+ 20 μg / g GHK-Cu. Except for the control group given air inhalation, the other three groups were given cigarette smoke exposure. The mice were placed in a 300L fume box, 20 cigarettes were lit each time, a...

Embodiment 2

[0023] Example 2 HE staining to determine the effect of GHK-Cu on CS-induced emphysema.

[0024] 1. Hematoxylin (hematoxylin) and eosin (eosin) staining (referred to as HE staining) is the most extensive staining method for nuclear histology. The basic principle is: the chromatin in the nucleus is mainly DNA. In the double-helix structure of DNA, the phosphate groups on both sides of the chain are outward, negatively charged, and acidic. bonded or hydrogen bonded. Hematoxylin is blue in alkaline solution, so cell nuclei are stained blue. The main component in the cytoplasm is protein, which is an amphoteric compound, and the staining of the cytoplasm is closely related to the pH value. Eosin is a chemically synthesized acid dye that dissociates into negatively charged anions in water and combines with positively charged amino groups (cations) of proteins to stain cytoplasm, cytoplasm, red blood cells, muscles, connective tissues, and eosinophilic granules etc. are infected ...

Embodiment 3

[0037] Example 3 The expression of inflammatory factors IL1β and TNFα in BALF was detected by ELISA.

[0038] Take out the reagents and equilibrate to room temperature. Mix the reagents and samples thoroughly and avoid air bubbles as much as possible.

[0039] 1. Adding samples: set up blank holes, standard holes, and sample holes to be tested respectively. Add 10 μl of sample diluent to the blank well, add 100 μl of standard substance or test sample diluted in different proportions to the remaining wells, cover the membrane, and incubate at 37°C for 2 hours.

[0040] 2. Discard the liquid, shake dry, add 100 μl of biotin antibody (1X) to each well, cover the membrane, and incubate at 37°C for 1 hour.

[0041] 3. Discard the liquid in the well, shake dry, wash the plate 3 times, soak for 1-2min each time, shake dry and pat dry the liquid in the well on absorbent paper.

[0042] 4. Add 100 μl of HRP-avidin (1X) to each well, cover the membrane, and incubate at 37°C for 1 hour...

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Abstract

The invention relates to the field of treatment of smoking-related respiratory system diseases, in particular to an application of oligopeptide and copper ion chelate thereof in prevention and treatment of lung smoking-related respiratory system diseases. The invention discloses an application of the oligopeptide and the copper ion chelate thereof in preparation of a medicine for preventing and / ortreating the smoking-related respiratory system diseases. An amino acid sequence of the oligopeptide is Gly-His-Lys. Experiments prove that the GHK oligopeptide and the copper ion chelate thereof caninhibit the occurrence of smoking-related diseases of mice by antagonizing oxidative stress reaction and inflammatory reaction, and inhibit the occurrence of collagen deposition and airway remodelingaround the airway by antagonizing epithelial mesenchymal transition, thereby inhibiting the occurrence and development of the smoking-related respiratory diseases.

Description

technical field [0001] The invention relates to the field of treatment of smoking-related respiratory diseases, in particular to the application of a short peptide and its copper ion chelate in the prevention and treatment of lung smoking-related respiratory diseases. Background technique [0002] Smoking-related respiratory disease is a common, preventable and treatable disease whose main pathophysiological features are emphysema, chronic airway inflammation and airway remodeling, resulting in airflow limitation and progressive decline in lung function , seriously affecting the quality of life of patients. Many factors play an important role in the development of emphysema, chronic airway inflammation and airway remodeling, among which inflammation, oxidative stress and epithelial mesenchymal transition (EMT) are particularly important. At present, the treatment of smoking-related respiratory diseases mainly includes bronchodilators, glucocorticoids, theophylline, leukotri...

Claims

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Application Information

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IPC IPC(8): A61K38/06A61K33/34A61P11/00
CPCA61K38/06A61K33/34A61P11/00A61K2300/00
Inventor 侯刚邓明明杨汀
Owner CHINA JAPAN FRIENDSHIP HOSPITAL
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