A kind of determination method of arsenic content in rice
A determination method and rice technology, applied in the field of chemical analysis and detection, can solve the problems of not being suitable for popular use, wide linear range, expensive equipment, etc., and achieve the effect of convenient detection method, low detection limit and stable aqueous solution
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Embodiment 1
[0034] Preparation of probe molecules: 2-aminopyridine (0.0941 g, 1 mmol) and salicylaldehyde (0.1710 g, 1.4 mmol) were added into 5 mL of anhydrous methanol, and refluxed at 65° C. for 6 hours. After cooling, the obtained solid product was refrigerated and recrystallized at 4° C., and filtered after the solid crystal was obtained. The crude fluorescent probe molecules obtained by filtration are then washed repeatedly with 5mL petroleum ether, in order to absorb unreacted compounds adsorbed on the solid surface. Then, the crystalline sample was separated and purified with a silica gel column (eluent: petroleum ether / ethyl acetate=5:1 volume ratio); the yellow crystal product finally obtained was NAHPL. The single crystal structure diagram and optimization diagram of the fluorescent probe molecule NAHPL are shown in figure 1 and figure 2 .
[0035] Prepare chromogen solution: Dissolve 0.01mmol of NAHPL into 0.5mL of anhydrous methanol, then dilute the solution to 2mL with d...
Embodiment 2
[0040] In the same manner as in Example 1, the probe molecules were prepared, the developer solution was prepared, and the standard card was prepared. Take 3ml of the prepared fluorescent chromogen solution and transfer it to a micro sampler, then add the arsenic-containing aqueous solution of the rice growth environment and mix well. The color was developed by irradiation with 365nm ultraviolet light for 5min. And compared with the standard color card, the degree of arsenic pollution can be determined.
[0041] As can be seen from the results, under the irradiation of 365nmn ultraviolet light, the chromogen solution without arsenic ions does not have obvious fluorescence phenomenon. However, after adding the sample to be tested, the fluorescence of the NAHPL fluorescent probe molecule was significantly enhanced. It shows that arsenic ions have a great influence on the fluorescence intensity of NAHPL fluorescent probe molecules, so the NAHPL fluorescent probe molecules can b...
Embodiment 3
[0043] In the same manner as in Example 1, the probe molecules were prepared, the developer solution was prepared, and the standard card was prepared. Take 8 parts of 2ml NAHPL solution into PU tubes, number them sequentially, add the same amount of heavy metal cations Na ions, Cd ions, Fe ions, Ca ions, K ions, Mg ions, Cu ions, As ions in the rice growth environment aqueous solution, except Except for the last PU tube, the rest do not contain arsenic. The above 8 PU tubes were used as control samples for color contrast experiments. Take another 7 parts of 2ml NAHPL solution into PU tubes, number them sequentially, and add the same amount of heavy metal cations Na ions, Cd ions, Fe ions, Ca ions, K ions, Mg ions, and Cu ions to the rice growth environment containing arsenic ions Aqueous solution, the above 7 PU tubes are test samples for anti-interference experiment.
[0044] The prepared samples were first placed under dark light for pre-reaction and mixed evenly. The trea...
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