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Method for extracting chitin from lucid ganoderma waste

A technology of waste and chitin, which is applied in the field of new technology, can solve the problems of excessive consumption of hydrochloric acid and sodium hydroxide, allergic reactions, and high production costs, and achieve the effects of reducing environmental pollution, easy handling, and reduced reaction time

Active Publication Date: 2021-04-06
SOUTH CHINA UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the disadvantages of high production cost, large environmental pollution, high consumption of hydrochloric acid and sodium hydroxide, long reaction time, and allergic reactions in chitin prepared from shrimp and crab shells in the traditional process, the present invention proposes a chitin obtained from shrimp and crab shells. Method for extracting chitin from Ganoderma lucidum waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Powdering: take the wastes of Ganoderma lucidum after extracting polysaccharides with water, dry them, and powder them to obtain Ganoderma lucidum powder.

[0039] (2) Wall breaking: take 10 g of ganoderma lucidum powder and put it into a container, add 150 ml of 2% hydrochloric acid solution, mix evenly, put it in an oil bath at 90° C. and stir for 2 hours to obtain a solution.

[0040] (3) One-time cleaning: centrifuge the solution after hydrochloric acid wall breaking in step (2), and carry out two cleanings with deionized water to obtain filter residue.

[0041] (4) Sodium hydroxide deproteinization: put the filter residue obtained in step (3) into a container, add 150ml of 8% sodium hydroxide solution, mix well, put it in an oil bath at 90°C and stir for 2h to obtain a solution.

[0042] (5) Secondary cleaning: the solution after sodium hydroxide deproteinization in step (4) is subjected to secondary centrifugation, and is washed with deionized water for 3 time...

Embodiment 2

[0047] (1) Powdering: take the wastes of Ganoderma lucidum after extracting polysaccharides with water, dry them, and powder them to obtain Ganoderma lucidum powder.

[0048] (2) Wall breaking: take 30g of Ganoderma lucidum powder and put it into a container, add 300ml of 3% hydrochloric acid solution, mix evenly, put it in an oil bath at 100°C and stir for 1 hour to obtain a solution.

[0049] (3) One-time cleaning: centrifuge the solution after hydrochloric acid wall breaking in step (2), and perform one-time cleaning with deionized water to obtain filter residue.

[0050] (4) Sodium hydroxide deproteinization: put the filter residue obtained in step (3) into a container, add 300ml of 10% sodium hydroxide solution, mix well, put it in an oil bath at 100°C and stir for 1 hour to obtain a solution.

[0051] (5) Secondary cleaning: the solution after sodium hydroxide deproteinization in step (4) was subjected to secondary centrifugation, and was washed twice with deionized wate...

Embodiment 3

[0056] (1) Powdering: take the wastes of Ganoderma lucidum after extracting polysaccharides with water, dry them, and powder them to obtain Ganoderma lucidum powder.

[0057] (2) Wall breaking: put 45g of ganoderma lucidum powder into a container, add 265ml of 4.5% hydrochloric acid solution, mix well, put into an oil bath at 120°C and stir for 1.5h to obtain a solution.

[0058] (3) One-time cleaning: centrifuge the solution after hydrochloric acid wall breaking in step (2), and clean it three times with deionized water to obtain a filter residue.

[0059] (4) Sodium hydroxide deproteinization: put the filter residue obtained in step (3) into a container, add 265ml of 17.5% sodium hydroxide solution, mix well, put it in an oil bath at 115° C. and stir for 2 hours to obtain a solution.

[0060] (5) Secondary cleaning: the solution after sodium hydroxide deproteinization in step (4) is subjected to secondary centrifugation, and is washed with deionized water for 3 times to obta...

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PUM

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Abstract

The invention discloses a method for extracting chitin from lucid ganoderma waste, and the method comprises the following steps: (1) drying the waste after polysaccharide water extraction of lucid ganoderma, and performing pulverizing to obtain lucid ganoderma powder; (2) adding a hydrochloric acid solution into the lucid ganoderma powder, performing uniform mixing, heating, stirring and reacting to obtain a solution, centrifuging the solution, and cleaning with deionized water to obtain filter residues; (3) adding a sodium hydroxide solution into the filter residues, performing uniform mixing, heating, stirring and reacting to obtain a solution, centrifuging the solution, and cleaning with deionized water to obtain filter residues; (4) adding a hydrogen peroxide solution into the filter residues obtained in the step (3), performing uniform mixing, heating, stirring and reacting to obtain a solution, centrifuging the solution, and cleaning with deionized water to obtain filter residues; and carrying out freeze drying on the filter residues to obtain chitin. According to the method, the chitin extraction rate is 6%-8% and is about two times that of a conventional extraction method, the ash content is low, a decalcification process can be omitted, the method is simple, and the process is controllable.

Description

technical field [0001] The invention relates to a method for extracting chitin, in particular to a new process for extracting chitin from Ganoderma lucidum waste. Background technique [0002] Chitin, also known as chitin, chitin, β-(1,4)-2-acetylamino-2-deoxy-D-glucose, molecular formula (C 8 h 13 NO 5 )n is a linear polymer polysaccharide linked by β-1,4 glycosidic bonds. In nature, it widely exists in the exoskeletons of arthropods, especially crustaceans and insects. In addition, it also widely exists in the cell walls of microorganisms such as fungi, mollusk endoskeletons and algae. Because chitin is natural, non-toxic, has good biocompatibility, degradability, and unique molecular structure and physical and chemical properties, it is widely used in medicine, pharmaceutical preparation, chemical industry, food, cosmetics, printing and dyeing. , papermaking, agriculture, environmental protection, and enzyme immobilization carriers have a wide range of uses. At pres...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/003C08B37/0003
Inventor 程建华蔡文婷
Owner SOUTH CHINA UNIV OF TECH