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Preparation method of edible mushroom calcareous sand breeder seeds and application

An edible fungus and calcium technology, applied in the fields of botanical equipment and methods, application, horticulture, etc., can solve the problems of cumbersome production process, high pollution rate, difficult to quantify, etc.

Inactive Publication Date: 2021-04-09
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides the preparation and application of an edible fungus calcareous sand original seed. Usually, the edible fungus original seed is mostly made of cereal raw materials, among which wheat and millet are the most common. The production process of such original species is cumbersome and difficult to quantify. There are many problems such as high pollution rate, long germination time, easy aging of bacteria, etc.

Method used

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  • Preparation method of edible mushroom calcareous sand breeder seeds and application
  • Preparation method of edible mushroom calcareous sand breeder seeds and application
  • Preparation method of edible mushroom calcareous sand breeder seeds and application

Examples

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Comparison scheme
Effect test

Embodiment 1

[0034] A kind of edible fungus calcareous sand original seed preparation is characterized in that it is that calcareous sand is through pulverizing and sieving sorting, and particle size is 1-2mm; PDA liquid culture medium solution after improvement: potato flour 6g / L, glucose 20g / L L, KH 2 PO 4 3g / L,MgSO 4 ·7H 2 O 1.5g / L Soak for 12 hours, control the water until there is no liquid flow, add wheat bran according to 5% of the dry weight of the calcareous sand, mix well, bottle and sterilize.

Embodiment 2

[0036] The preparation method of edible mushroom calcareous sand former seed is to carry out as follows:

[0037] (1) Crush the calcareous sand, remove sundries, and then sieve and sort. The particle size is 1-2mm. Strictly control the pore size of the particles, pack them in a dry environment and store them for later use;

[0038] (2) Weigh the calcareous sand after screening and add it into the improved PDA liquid medium according to 1:1. The main components of the improved PDA liquid medium are potato flour 6g / L, glucose 20g / L, KH 2 PO 4 3g / L, MgSO 4 ·7H 2 O 1.5g / L, pH is natural, so that the calcareous sand is in full contact with the improved PDA liquid medium for 12 hours, and the ambient temperature should not exceed 20°C, so as not to breed bacteria. After soaking for 12 hours, remove the calcareous sand and control the water until there is no liquid flow. 10% weight gain, too high water content, too low nutrition and insufficient nutrition are not conducive to the...

Embodiment 3

[0044] Use a wide-mouth bottle as a container, fill about 200g of calcium sand, soak the improved PDA liquid medium, add 10g of wheat bran, the wet weight is about 0.22kg, and do 10 repetitions for each group of experiments, based on this, respectively according to ( 0, 5%, 10%, 15%, 20%, 25%, 30%) add wheat bran, mix thoroughly, stuff for 0.5h, pack into bags, sterilize at 121°C for 0.5h, inoculate after cooling, the test results show Except for 0 gradient, mycelia in other gradient ranges can germinate, and the higher the gradient, the slower the growth. Therefore, 5% was selected as the addition amount of wheat bran in the production of edible fungus calcareous sand original species in the experiment.

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Abstract

The invention provides a preparation method of edible mushroom calcareous sand breeder seeds and application thereof. According to the preparation method, calcareous sand is sieved and soaked in a liquid culture medium, then wheat bran is added according to a certain proportion, an edible fungus cultivar culture medium is prepared to replace cereal formula breeder seeds such as wheat, the requirement for common cereal raw materials such as the wheat for breeder seed preparation in the edible fungus industry is reduced while the strain activity is not affected, the production cost is reduced, and the pollution rate is reduced; and the preparation process is simplified, the spawn running time is shortened, the strain preservation period is prolonged, and an effective method is provided for the preparation of the edible mushroom breeder seeds.

Description

technical field [0001] The invention belongs to the technical field of edible fungus strain development, and relates to the preparation of edible fungus strains in the upstream of the edible fungus industry chain, more specifically, a preparation method and application of edible fungus calcareous sand original seeds. Background technique [0002] At present, the total output of edible fungi in my country ranks first in the world, and the total output in 2020 is expected to reach 40.812 million tons, of which the total industrial production accounts for about 10%, and the rest is the production of edible fungi under the facility mode, non-industrial production of edible fungi The strains are still mainly solid strains. Due to the high technical requirements for liquid strains and the high cost of equipment, solid strains are still used in individual factories. Among the solid strains, the original strains mostly use wheat or millet as the main raw material, and Bailing Take mus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/00
CPCA01G18/20A01G18/00
Inventor 刘西周
Owner TIANJIN NORMAL UNIVERSITY