Application of Dipsacus saponin B in the preparation of drugs for preventing and treating vascular diseases
A kind of technology of saponin B and medicine, applied in the field of medicine
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Example 1 Prediction of network pharmacology on the effect of saponin B on DNA topoisomerase 2a (TOP2a)
[0028] The saponin B used in the embodiment of the present invention was purchased from Nanjing Chunqiu Biological Co., Ltd., and the purity was >98%. Download the structural formula of saponin B from the pubchem database, with a similar score > 0.8 in the ChemMapper database, and the conditions of the BindingDB biological activity library predict the target of saponin B, and download the predicted target. The IGEMDOCK molecular docking software was used to dock saponin B with the target. First, select the active docking site, input the compound for docking, and analyze and score to determine the follow-up research object. The database prediction results are shown in Table 1 and figure 1 .
[0029] Table 1 ChemMapper database to predict the target of saponin B
[0030]
[0031] The results showed that the values of TOP2a, BCL-xL, PYGM and PTGS1 in iGEMDOCK d...
Embodiment 2
[0032] Example 2 Experiment on the inhibition of Ang-II-induced VSMCs cell proliferation by Chuanxu saponin B
[0033] The cell viability was detected by MTT method, and the logarithmic growth phase VSMCs were taken and plated at a density of 8000 cells / well. At the same time, an equal volume of PBS was added to the peripheral wells to avoid the marginalization effect. After 24h, dissolve the saponin B with culture medium, gradient such as: 1, 3, 10, 30, 100, 300, 1000 μM, each empty 100 μL, incubate for 2 hours, then discard, add and then add angiotensin II (Angiotensin II, Ang-II) (1 μM) to induce the proliferation model, incubate together for 24 h, discard, add 100 μL of MTT solution to each well, and continue to incubate for 4 h. Then aspirate the culture medium, add 150 μL DMSO, shake for 10 min on a shaker, and detect the OD value at a wavelength of 570 nm on a microplate reader. see the results figure 2 .
[0034] The results showed that compared with the control g...
Embodiment 3
[0037] Example 3 Chuanxu saponin B inhibits the expression of VSMCs cell proliferation-related proteins induced by Ang-II
[0038] The cells were seeded in a petri dish at 5×105 / ml, and when the cell confluence reached 80%, the cells were treated in the following corresponding groups for 24 hours. The specific grouping is as follows: control group (high glucose medium), model is Ang-II (1 μmol / L) treatment group, positive control is curcumin (Curcumin, Cur) treatment group (Cur, 5 μmol / L) + Ang-II , Chuanxuduansaponin B treatment group Chuanxuduansaponin B (3, 10, 30 μmol / L) + Ang-II. Cell proteins were collected by lysis, and a 10% SDS-polyacrylamide gel was prepared using an SDS-PAGE gel preparation kit; Electrophoresis was performed at 100V for 100 minutes, the gel was placed on the PVDF membrane and placed on a wet transfer membrane, the current was 300mA, and the time was 2 hours. PVDF was placed in TBS-T containing 5% nonfat milk powder, at room temperature for 45 minu...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More