Check patentability & draft patents in minutes with Patsnap Eureka AI!

Gas chromatography detection method of dicyclohexylamine in favipiravir

A technology of dicyclohexylamine and gas chromatography, which is applied in the field of gas chromatography detection of dicyclohexylamine content in favipiravir, can solve the problems of low recovery rate of dicyclohexylamine, achieve good reproducibility and eliminate reaction Adsorption, accurate results

Active Publication Date: 2021-04-13
ZHEJIANG HISUN PHARMA CO LTD
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problem of low recovery of dicyclohexylamine in conventional gas chromatography detection methods, the invention provides a gas chromatography detection method of dicyclohexylamine in favipiravir with short analysis time, high sensitivity and good reproducibility

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gas chromatography detection method of dicyclohexylamine in favipiravir
  • Gas chromatography detection method of dicyclohexylamine in favipiravir
  • Gas chromatography detection method of dicyclohexylamine in favipiravir

Examples

Experimental program
Comparison scheme
Effect test

preparation example

[0031] Chromatographic conditions: Agilent HP-5 gas chromatography column, the stationary phase is (5%-phenyl)-methylpolysiloxane, the specification: 30m×0.53mm×5.00μm, the flow rate is 4.0ml / min, and the split ratio is 1 : 1; temperature programmed to 40°C for 3 minutes, 10°C / min to 200°C, and hold for 5 minutes; 20°C / min to 220°C, and hold for 10 minutes;

[0032] The diluent is N,N dimethylacetamide;

[0033] Preparation of linear dicyclohexylamine solution: Take 16 mg of dicyclohexylamine, accurately weigh it and set it in a 100ml volumetric flask, dissolve it with a diluent and dilute to the mark, mix well; accurately transfer 2.0ml into a 100ml volumetric flask, and dilute to Scale, mix well; then pipette 2.0ml, 5.0ml, 8.0ml, 10.0ml, 15.0ml precisely, place them in four 20ml volumetric flasks, dilute to the mark with diluent, mix well; get 0.32, 0.80, 1.28 , 1.60, 2.40, 3.20 μg / ml linear solution; precisely pipette 1.0ml of each linear solution into the injection bottle...

Embodiment 1

[0055] Chromatographic conditions: Agilent HP-5 gas chromatography column, the stationary phase is (5%-phenyl)-methylpolysiloxane, the specification: 30m×0.53mm×5.00μm, the flow rate is 4.0ml / min, and the split ratio is 1 : 1; temperature programmed at 40°C for 3 minutes, 10°C / min to 200°C, and held for 12 minutes; diluent: N,N dimethylacetamide;

[0056] Dicyclohexylamine control solution: prepare 1.6 μg / ml dicyclohexylamine solution, take 1.0ml into the sample bottle, then add 100μl of 30% sodium hydroxide solution and mix well;

[0057] Sample solution: Weigh 80mg of favipiravir sample and add 1.0ml of diluent N,N dimethylacetamide to dissolve, then add 100μl of 30% sodium hydroxide solution and mix well. Clear liquid injection;

[0058] Dicyclohexylamine sample recovery experiment: Weigh 80 mg of favipiravir sample, add 1.0ml of dicyclohexylamine control solution to dissolve; then add 100 μl of 30% sodium hydroxide solution and mix well. After shaking, a large amount of s...

Embodiment 2

[0060] Embodiment 2: compare the volume of adding lye, place time, place temperature parameter comparison

[0061] Chromatographic conditions Agilent HP-5 gas chromatography column, the stationary phase is (5%-phenyl)-methylpolysiloxane, the specification: 30m×0.53mm×5.00μm, the flow rate is 4.0ml / min, and the split ratio is 1: 1. Program temperature rise to 40°C for 3 minutes, 10°C / min to 200°C and hold for 12 minutes; diluent: N,N dimethylacetamide;

[0062] The first group: dicyclohexylamine control solution: prepare 1.6μg / ml dicyclohexylamine solution; take 1.0ml of this solution, add 60mg solid NaOH, seal and shake;

[0063] Sample solution: Weigh 80mg of favipiravir sample, add 1.0ml N,N dimethylacetamide to dissolve; add 60mg solid NaOH, seal and shake;

[0064] Dicyclohexylamine sample recovery solution: weigh 80 mg of favipiravir sample, add 1.0ml of dicyclohexylamine reference solution to dissolve; add and weigh 60mg of solid NaOH, seal and shake.

[0065] The seco...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a gas chromatography detection method of dicyclohexylamine in favipiravir, which is short in analysis time, high in sensitivity and good in reproducibility. According to the method, a 30% sodium hydroxide solution with the mass percent concentration unit of g / g is adopted, heated and placed, interference of a matrix on the detected object dicyclohexylamine can be eliminated, the linearity is good, and the quantification is accurate; the 30% sodium hydroxide solution is added to eliminate the reaction adsorption effect of phenolic hydroxyl groups on the detected object, a sample is introduced after being alkalized and placed, the result is accurate, and the reproducibility is good.

Description

technical field [0001] The invention relates to the field of chemical analysis and detection, in particular to a gas chromatography detection method for dicyclohexylamine content in favipiravir. Background technique [0002] Favipiravir (Favipiravir, T-705), chemically named 6-fluoro-3-hydroxypyrazine-2-carboxamide, is a novel RNA-dependent RNA polymerase (RdRp) inhibitor class broad-spectrum antiviral drug, It has a good therapeutic effect on type A influenza (including avian influenza and influenza A H1N1 infection) virus, and its structural formula is as follows [0003] [0004] The dicyclohexylamine used in the synthetic route of favipiravir is a flammable and highly toxic substance. It is very important to carry out accurate and rapid quantitative detection of these toxic substances in the production process so that they can be effectively removed in the product. Control product quality. The conventional gas chromatographic detection method of dicyclohexylamine co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/54G01N30/88
CPCG01N30/02G01N30/06G01N30/54G01N30/88G01N2030/025G01N2030/062G01N2030/884
Inventor 金美春郑朝阳陈延安
Owner ZHEJIANG HISUN PHARMA CO LTD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com