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Application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise tolerance

A technology of conjugated linoleic acid and skeletal muscle, applied in the field of skeletal muscle exercise, to improve exercise endurance and meat quality, increase running time and distance, and enhance exercise endurance

Pending Publication Date: 2021-04-20
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise tolerance
  • Application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise tolerance
  • Application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise tolerance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Adding 1% trans-10cis-12 conjugated linoleic acid to the diet can increase the running time and distance of mice

[0032] 1. In order to study the effect of trans-10-cis-12 conjugated linoleic acid monomer on the exercise endurance of mouse skeletal muscle, 8-week-old male C57BL / 6 mice were used as the research object.

[0033] 2. Experimental method

[0034] (1) Animal feeding method: 1 animal / box, single breeding; feeding temperature and humidity: 22-26°C, 40%-70%; 12h: 12h day and night intermittent lighting; feeding conditions are always kept stable to ensure the accuracy of the test results reliability. During the breeding period, the animals had free access to food and drinking water, and the feed and drinking water were provided by Guangdong Medical Experimental Animal Center. The purchased SPF grade C57BL / 6 mice were pre-fed for 7 days. Animals were checked once a day during this period.

[0035](2) Animal grouping and treatment: 8-week-old mice w...

Embodiment 2

[0041] Example 2: Adding 1% trans-10cis-12 conjugated linoleic acid to the diet increases the content and proportion of type I muscle fibers in the gastrocnemius muscle of mice

[0042] 1. In order to study the effect of trans-10-cis-12 conjugated linoleic acid on the muscle fiber types of mice, 8-week-old male C57BL / 6 mice were used as the research objects.

[0043] 2. Experimental method

[0044] (1) Animal breeding method: the same as in Example 1.

[0045] (2) Grouping and treatment of animals: same as in Example 1.

[0046] (3) Sampling and detection: The experiment was carried out for 16 weeks. On the day of the end of the experiment, the mice were sacrificed by cervical dislocation, and the gastrocnemius muscles of all mice were collected, sectioned for immunofluorescent staining, and protein extracted for Western Blot experiment. To examine the effects of dietary supplementation of conjugated linoleic acid on muscle fiber types in mice.

[0047] 3. The result is as ...

Embodiment 3

[0051] Example 3: Adding 1% trans-10cis-12 conjugated linoleic acid to the diet improves the enzyme activities related to mitochondrial respiration and oxidative metabolism in mouse gastrocnemius muscle

[0052] 1. In order to study the effect of adding 1% trans-10cis-12 conjugated linoleic acid in the diet on the mitochondrial respiration and oxidative metabolism-related enzyme activities of mouse gastrocnemius muscle, the experiment took 8-week-old male C57BL / 6 mice as the research objects.

[0053] 2. Experimental method

[0054] (1) Animal breeding method: the same as in Example 1.

[0055] (2) Grouping and treatment of animals: same as in Example 1.

[0056] (3) Sampling and detection: The experiment was carried out for 16 weeks. On the day of the end of the experiment, the mice were killed by cervical dislocation, blood was collected from the eyeballs, the gastrocnemius muscle was separated, and the supernatant was obtained from the homogenate to detect mitochondrial re...

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Abstract

The invention belongs to the technical field of skeletal muscle exercises, and discloses an application of trans-10 cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise tolerance. The new application of the trans-10 cis-12 conjugated linoleic acid is provided, and the trans-10 cis-12 conjugated linoleic acid can significantly increase the running exercise time and distance of animals, increase the contraction duration of skeletal muscles of the animals and enhance the exercise tolerance of the skeletal muscles. The trans-10 cis-12 conjugated linoleic acid enhances mitochondrial respiration of the skeletal muscles of animals and oxidative metabolism related enzyme activity of the skeletal muscles, and improves skeletal muscle I-type fiber types, so that the exercise tolerance of the skeletal muscles is remarkably enhanced. The trans-10 cis-12 conjugated linoleic acid does not contain substances harmful to animals or human bodies, is low in addition amount, can be directly added into animal feed, food or medicine, has the advantages of safety, effectiveness, no toxic or side effect and the like, and has good popularization and application prospects in the aspect of enhancing exercise tolerance of the skeletal muscles.

Description

technical field [0001] The invention belongs to the technical field of skeletal muscle exercise, and in particular relates to an application of trans-10-cis-12 conjugated linoleic acid in enhancing skeletal muscle exercise endurance. Background technique [0002] At present, skeletal muscle accounts for about 40-60% of the body weight of an adult animal. It is a kind of striated muscle in muscle tissue, which is composed of thousands of contractile muscle cells, which are called muscle fibers. During development, myotubes are formed by fusion of mesoderm progenitor cells, which redifferentiate to form myofibers. Under normal circumstances, the number of muscle fibers is stable before the birth of animals, and the growth of muscles in the postnatal stage is mainly reflected in the changes in the size and type of muscle fibers, and the transformation of muscle fiber types occurs throughout the growth and development process. Muscle fibers can be divided into different types a...

Claims

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Application Information

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IPC IPC(8): A23K20/158A23K50/50A23L33/12
Inventor 王松波束刚江青艳段晨朱晓彤王丽娜高萍
Owner SOUTH CHINA AGRI UNIV
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