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Microalgae culture medium and application thereof

A medium and microalgae technology, applied in microorganisms, unicellular algae, microorganism-based methods, etc., can solve the problems of increased cost of microalgae cultivation, high cost, and inability to meet commercialization needs.

Active Publication Date: 2021-04-20
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of cultivating microalgae with traditional microalgae medium, there are the following problems. On the one hand, the cost of traditional microalgae medium is relatively high, resulting in an increase in the cost of microalgae culture; on the other hand, the value-added rate of microalgae and the polysaccharide The yield is low and cannot meet the current commercial needs

Method used

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  • Microalgae culture medium and application thereof
  • Microalgae culture medium and application thereof
  • Microalgae culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The embodiment of the application provides the collection and hydrolysis test of kitchen waste, the specific steps are as follows:

[0064] 1. Kitchen waste is collected in the canteen of Jinan University in Guangzhou, mainly including noodles, steamed buns, bread and rice. The collected food waste was dried overnight in an oven at 60°C and then stored at -20°C.

[0065] 2. 300g of dried food waste was crushed with a blender and put into a 2.5L hydrolysis tank, the reaction volume was adjusted to 2.0L with pure water, and 10mL of commercial glucoamylase was added and then heated at 55°C and 300rpm Under hydrolysis for 24 hours, recorded as the sample solution. During the hydrolysis process, the pH of the sample solution was kept at about 4.0 with an acid regulator.

[0066] 3. During the 24 hours of enzymatic hydrolysis, sample 1 mL from the bioreactor at regular intervals for glucose content determination; after the end of enzymatic hydrolysis, also sample 1 mL for g...

Embodiment 2

[0070] The embodiment of the present application provides an analysis experiment on the food waste and the food waste hydrolyzate obtained in Example 1. The specific steps are as follows:

[0071] The total sugar content of food waste and food waste hydrolyzate was detected by phenol-sulfuric acid method: collect 2g of food waste and 2mL of food waste hydrolyzate obtained in Example 1, use Ningbo Xinzhi Freeze Dryer for 48 hours and put into a 50mL centrifuge tube, and add 1mL of deionized water to the tube. After vortexing for 30 s, add 1 mL of 5% phenol solution to the centrifuge tube, and then slowly add 5 mL of 98% concentrated sulfuric acid along the tube wall. Incubate in a water bath at 25°C for 10 min, then transfer to a water bath at 30°C for 20 min. Using commercial pure glucose as a standard product, after following the above steps, add the standard product reaction solution, food waste reaction solution, and food waste hydrolyzate reaction solution into the pre-ar...

Embodiment 3

[0080] The embodiment of the present application provides the preparation of different microalgal culture media, and the specific steps are as follows:

[0081] Preparation of microalgae culture medium with different concentrations of food waste hydrolyzate: the preparation includes fresh water, KH 2 PO 4 0.02g / L, MgSO 4 ·7H 2 O 0.025g / L, vitamin B12 0.5μg / L, potassium citrate 0.04g / L, vitamin B1 0.4mg / L microalgae medium without carbon source; Add 10g / L, 20g / L, 30g / L, 40g / L of food waste hydrolyzate to the algae culture medium; prepare microalgae culture medium containing different concentrations of food waste hydrolyzate.

[0082] Preparation of microalgae culture medium with different concentrations of crude glycerol: the preparation includes fresh water, KH 2 PO 4 0.02g / L, MgSO 4 ·7H 2 O 0.025g / L, vitamin B12 0.5μg / L, potassium citrate 0.04g / L, vitamin B1 0.4mg / L microalgae medium without carbon source; Add 100mM, 200mM, 300mM, 400mM crude glycerol to the algae c...

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Abstract

The invention belongs to the technical field of microalgae culture, and particularly relates to a microalgae culture medium and application thereof. The first aspect of the invention provides the microalgae culture medium. The microalgae culture medium comprises a carbon source, KH2PO4, MgSO4.7H2O, potassium citrate, vitamin B1, vitamin B12 and water, wherein the carbon source is selected from kitchen waste hydrolysate and / or glycerol products; and the water is selected from seawater or fresh water. The second aspect of the invention further discloses application of the microalgae culture medium in promoting microalgae proliferation and promoting microalgae polysaccharide accumulation. According to the microalgae culture medium and the application thereof, the accumulation of microalgae biomass and microalgae polysaccharide can be remarkably promoted, and the microalgae culture medium is high in microalgae culture efficiency, low in cost and wide in industrial application prospect.

Description

technical field [0001] The application belongs to the technical field of microalgae cultivation, and in particular relates to a microalgae culture medium and its application. Background technique [0002] Microalgae are a kind of autotrophic plants widely distributed in land and ocean, rich in nutrients and high in photosynthetic utilization. The polysaccharides, proteins, pigments, etc. produced by cell metabolism make them useful in the fields of food, medicine, genetic engineering, and liquid fuels. Very good development prospects. The individual sizes of algae vary greatly, and among them, the microalgae group whose shape can only be distinguished under a microscope is called microalgae (microalgae), so microalgae is not a taxonomic name. [0003] Microalgae are rich in a variety of nutrients, such as Euglena, which is rich in a variety of excellent ingredients, including Euglena, amino acids, unsaturated fatty acids, vitamins and antioxidant components. Among them, eu...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P19/04C12R1/89
Inventor 李宏业汪翔刘思芬杨维东
Owner JINAN UNIVERSITY
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