Microalgae culture medium and application thereof
A medium and microalgae technology, applied in microorganisms, unicellular algae, microorganism-based methods, etc., can solve the problems of increased cost of microalgae cultivation, high cost, and inability to meet commercialization needs.
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Embodiment 1
[0063] The embodiment of the application provides the collection and hydrolysis test of kitchen waste, the specific steps are as follows:
[0064] 1. Kitchen waste is collected in the canteen of Jinan University in Guangzhou, mainly including noodles, steamed buns, bread and rice. The collected food waste was dried overnight in an oven at 60°C and then stored at -20°C.
[0065] 2. 300g of dried food waste was crushed with a blender and put into a 2.5L hydrolysis tank, the reaction volume was adjusted to 2.0L with pure water, and 10mL of commercial glucoamylase was added and then heated at 55°C and 300rpm Under hydrolysis for 24 hours, recorded as the sample solution. During the hydrolysis process, the pH of the sample solution was kept at about 4.0 with an acid regulator.
[0066] 3. During the 24 hours of enzymatic hydrolysis, sample 1 mL from the bioreactor at regular intervals for glucose content determination; after the end of enzymatic hydrolysis, also sample 1 mL for g...
Embodiment 2
[0070] The embodiment of the present application provides an analysis experiment on the food waste and the food waste hydrolyzate obtained in Example 1. The specific steps are as follows:
[0071] The total sugar content of food waste and food waste hydrolyzate was detected by phenol-sulfuric acid method: collect 2g of food waste and 2mL of food waste hydrolyzate obtained in Example 1, use Ningbo Xinzhi Freeze Dryer for 48 hours and put into a 50mL centrifuge tube, and add 1mL of deionized water to the tube. After vortexing for 30 s, add 1 mL of 5% phenol solution to the centrifuge tube, and then slowly add 5 mL of 98% concentrated sulfuric acid along the tube wall. Incubate in a water bath at 25°C for 10 min, then transfer to a water bath at 30°C for 20 min. Using commercial pure glucose as a standard product, after following the above steps, add the standard product reaction solution, food waste reaction solution, and food waste hydrolyzate reaction solution into the pre-ar...
Embodiment 3
[0080] The embodiment of the present application provides the preparation of different microalgal culture media, and the specific steps are as follows:
[0081] Preparation of microalgae culture medium with different concentrations of food waste hydrolyzate: the preparation includes fresh water, KH 2 PO 4 0.02g / L, MgSO 4 ·7H 2 O 0.025g / L, vitamin B12 0.5μg / L, potassium citrate 0.04g / L, vitamin B1 0.4mg / L microalgae medium without carbon source; Add 10g / L, 20g / L, 30g / L, 40g / L of food waste hydrolyzate to the algae culture medium; prepare microalgae culture medium containing different concentrations of food waste hydrolyzate.
[0082] Preparation of microalgae culture medium with different concentrations of crude glycerol: the preparation includes fresh water, KH 2 PO 4 0.02g / L, MgSO 4 ·7H 2 O 0.025g / L, vitamin B12 0.5μg / L, potassium citrate 0.04g / L, vitamin B1 0.4mg / L microalgae medium without carbon source; Add 100mM, 200mM, 300mM, 400mM crude glycerol to the algae c...
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