Rh blood group antigen detection card and preparation method thereof
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A technology of antigen detection and rh blood type, which is applied in the direction of measuring devices, instruments, scientific instruments, etc.
Pending Publication Date: 2021-04-20
上海润普生物技术有限公司
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[0004] In view of the problem that there is no detection card for C, c, E, e antigen detection in the prior art,
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preparation example 1
[0052] Preparation of Antibody Diluent:
[0053] Weigh the specified amount of sodium dihydrogen phosphate 0.2g, disodium hydrogen phosphate 0.6g, maltose 4.71g, melezitose 2.35g, lentinan 0.94g, lecithin 8g, sodium chloride 2g, sodium citrate 1g, porol Dissolve 0.05 g of sham and 15 mg of natamycin in 1 L of water, adjust the pH of the solution to 7, and prepare the antibody diluent.
preparation example 2
[0055] Preparation of Antibody Diluent:
[0056] Weigh the specified amount of sodium dihydrogen phosphate 0.6g, disodium hydrogen phosphate 0.2g, maltose 5.71g, melezitose 5.71g, lentinan 0.58g, lecithin 6g, sodium chloride 1.67g, sodium citrate 3.33g, 0.01 g of poloxamer and 18 mg of natamycin were dissolved in 1 L of water, and the pH of the solution was adjusted to 6 to prepare an antibody diluent.
preparation example 3
[0058] Preparation of Antibody Diluent:
[0059] Weigh the specified amount of sodium dihydrogen phosphate 0.3g, disodium hydrogen phosphate 0.5g, maltose 4.09g, melezitose 4.09g, lentinan 0.82g, lecithin 7.5g, sodium chloride 1.75g, sodium citrate 1.75g , 0.04 g of poloxamer, and 16 mg of natamycin were dissolved in 1 L of water, and the pH of the solution was adjusted to 6.6 to prepare an antibody diluent.
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Abstract
The invention discloses an Rh blood type antigen detection card and a preparation method thereof, and belongs to the technical field of blood type determination. The detection card comprises eight micro-column gel tubes, wherein the eight gel tubes comprise two gel tubes containing monoclonal anti-C antibodies with IgM properties, two gel tubes containing monoclonal anti-c antibodies with IgM properties, two gel tubes containing monoclonal anti-E antibodies with IgM properties and two gel tubes containing monoclonal anti-e antibodies with IgM properties. The detection card provided by the invention can specifically detect four antigens in a sample, can accurately obtain the Rh blood type of a to-be-detected person, meets the requirements of clinical rapid detection, also has relatively long service life, and improves the market competitiveness of products.
Description
technical field [0001] The application relates to the field of blood type typing cards, in particular to a Rh blood group antigen detection card and a preparation method thereof. Background technique [0002] The ABO blood group system and the Rh blood group system are the two most important blood group systems for human beings. Their high immunogenicity makes them have important clinical significance. They are not suitable for emergency rescue, surgical treatment, anemia treatment, pre-blood transfusion inspection, etc. What is lacking, the correctness of the test results is directly related to the patient's life safety. [0003] According to the presence or absence of A antigen and / or B antigen on the surface of red blood cell membrane, the blood type is divided into four types: A, B, AB and O. The importance of the Rh blood group system in transfusion medicine is second only to the ABO blood group system. The main antigens of the Rh system are D, C, c, E, and e antigens,...
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