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Method for shortening hysteresis period of cytidine fermentation

A lag phase and cytidine technology, which is applied in the field of shortening the lag phase of cytidine fermentation, can solve the problems of slow growth, unfavorable industrial production, and long fermentation cycle, and achieve the effects of saving time, reducing production costs, and improving fermentation stability

Pending Publication Date: 2021-04-27
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the microorganisms used in the production of cytidine by fermentation are basically genetically engineered Escherichia coli, but after the induction of genetically engineered bacteria, there will be a period of slow growth, resulting in a long fermentation cycle, which is not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for shortening the lag period of cytidine fermentation, the specific steps are as follows:

[0015] 1. Activation of strains: Inoculate the original strains into solid medium on a slope for activation to obtain strains for fermentation;

[0016] 2. Seed culture: transplant the bacterial classification obtained in step 1 into the seed medium for cultivation to obtain the seed liquid; the formula of the seed medium is: glucose 10g / L, yeast powder 4g / L, citric acid 6g / L L, peptone 6g / L, isoleucine 0.1g / L, glutamic acid 0.5g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 2.0g / L, corn steep liquor 5ml / L, ferrous sulfate 10mg / L L, manganese sulfate 5mg / L, cobalt chloride 4mg / L, biotin 4mg / L and VB 1 4mg / L; culture conditions: 37°C, pH 7.0, dissolved oxygen controlled at 25%;

[0017] 3. Fermentation culture: inoculate the seed solution obtained in step 2 into the fermentation medium, and the fermentation culture formula is: glucose 30g / L, yeast powder 6...

Embodiment 2

[0019] A method for shortening the lag period of cytidine fermentation, the specific steps are as follows:

[0020] 1. Activation of strains: Inoculate the original strains into solid medium on a slope for activation to obtain strains for fermentation;

[0021] 2. Seed cultivation: transplant the fermented strains obtained in step 1 into the seed medium for cultivation to obtain the seed liquid; the formula of the seed medium is: glucose 30g / L, yeast powder 10g / L, citric acid 3g / L L, peptone 1g / L, isoleucine 0.1g / L, glutamic acid 0.5g / L, potassium dihydrogen phosphate 9g / L, magnesium sulfate 0.5g / L, corn steep liquor 20ml / L, ferrous sulfate 40mg / L L, manganese sulfate 10mg / L, cobalt chloride 1mg / L, biotin 1mg / L and VB 1 1mg / L; culture conditions: 37°C, pH 7.0, dissolved oxygen controlled at 25%;

[0022] 3. Fermentation culture: inoculate the seed solution obtained in step 2 into the fermentation medium, the fermentation culture formula is: glucose 10g / L, yeast powder 10g / L...

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Abstract

The invention relates to a method for shortening the hysteresis period of cytidine fermentation, and belongs to the technical field of fermentation engineering. The method mainly comprises the following steps: adding glutamic acid and isoleucine nutrients into a culture medium, and slowly flowing corn steep liquor after fermentation is started for 3-4 hours to shorten the hysteresis condition of a fermentation process. Compared with the original process, the method disclosed by the invention has the advantages that the fermentation period reaching the same yield is shortened to be within 36 hours, the time is saved by about 6-14 hours, the batch is increased, and the production cost is indirectly reduced.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for shortening the lag period of cytidine fermentation. Background technique [0002] Cytidine is an important pharmaceutical synthesis intermediate, which can be used to synthesize antiviral and antitumor drugs such as cytarabine, azacitidine, and cytarabine. At present, the microorganisms used in the production of cytidine by fermentation are basically genetically engineered Escherichia coli, but after the induction of genetically engineered bacteria, there will be a period of slow growth, resulting in a long fermentation cycle, which is not conducive to industrial production. Therefore, it is of great significance to find a method to shorten the slow growth. Contents of the invention [0003] In order to overcome the slow growth phenomenon of cytidine genetically engineered bacteria after induction, the object of the present invention i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/38C12R1/19
CPCC12P19/385
Inventor 曹华杰张兆坤李静
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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