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Application of intestinal flora in the detection of hepatic encephalopathy

A technology for hepatic encephalopathy and Escherichia coli, which is applied to the application field of intestinal flora in the detection of hepatic encephalopathy, can solve problems such as the relationship between intestinal flora that has not been disclosed in the literature, and achieve simple operation and high specificity , the effect of strong sensitivity

Active Publication Date: 2021-11-09
RUIAN PEOPLES HOSPITAL +1
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AI Technical Summary

Problems solved by technology

[0019] Currently, there is no published literature on the relationship between intestinal flora and hepatic encephalopathy

Method used

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  • Application of intestinal flora in the detection of hepatic encephalopathy
  • Application of intestinal flora in the detection of hepatic encephalopathy
  • Application of intestinal flora in the detection of hepatic encephalopathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0049] Example 2 Fluorescence quantitative PCR verification related flora

[0050] 1. Perform large-scale QPCR verification on the above-mentioned flora, and collect 30 cases of feces samples from healthy people and patients with central hepatic encephalopathy according to the sample collection method in Example 1.

[0051] 2. Extraction and quantification of fecal bacterial DNA

[0052] Bacterial DNA was extracted from stool samples using a stool genomic DNA extraction kit, and the operation steps were carried out according to the instructions;

[0053] Genomic DNA was detected by 1% agarose gel electrophoresis to determine the total DNA concentration and purity of the extracted fecal bacteria, and the mass concentration of DNA in all samples was unified to 100 mg / L.

[0054] 3. Real-time fluorescent quantitative PCR

[0055] 1) Primer design and synthesis

[0056] According to the sequence pair of the V3-V4 region of the bacterial 16SrRNA gene. Specific primers were desi...

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Abstract

The invention relates to the application of intestinal flora in the detection of hepatic encephalopathy, and the intestinal flora is enterohemorrhagic Escherichia coli, hemolytic twin coccus and Kenzian traumatococcus. The present invention discovers the level change of specific flora in hepatic encephalopathy for the first time. By detecting the change of specific flora, the diagnosis of hepatic encephalopathy can be realized. Using the microbial markers of the present invention for diagnosis has simple operation, high specificity, Strong sensitivity.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to the application of intestinal flora in the detection of hepatic encephalopathy. Background technique [0002] Hepatic encephalopathy (HE), also known as hepatic coma, refers to a syndrome of central nervous system dysfunction caused by severe liver disease and based on metabolic disorders. Its main clinical manifestations are disturbance of consciousness, abnormal behavior and coma. It varies with the type of liver disease, the degree of liver cell damage, the onset of the disease, and the different causes. Due to the different underlying diseases leading to hepatic encephalopathy, its clinical manifestations are also complex and changeable, and the variability of early symptoms is the characteristic of this disease. But there are also common manifestations: that is, it is reflected in neuropsychiatric symptoms and signs. It has both the manifestations ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/19C12R1/01
CPCC12Q1/689G01N2800/28
Inventor 黄鹏张宏宇龚方华沈秀微王丰伟吴淑娟
Owner RUIAN PEOPLES HOSPITAL
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