A method for simultaneously detecting fosfomycin and its diols by ion chromatography

An ion chromatography and fosfomycin technology, applied in the field of chemical substance analysis and detection, can solve the problem of inability to distinguish fosfomycin and fosfomycin diol, inability to detect and affect fosfomycin and fosfomycin diol. Accuracy of fosfomycin, etc., to achieve the effect of low detection limit, high sensitivity and good reproducibility

Active Publication Date: 2021-10-08
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

The method for detecting fosfomycin by ion chromatography proposed in Chinese patent document CN103728385A cannot distinguish fosfomycin and fosfomycin diol (fosfomycin and fosfomycin diol peak time are very similar), so In fosfomycin wastewater or other samples, when fosfomycin and fosfomycin diol coexist, this method not only cannot detect fosfomycin and fosfomycin diol at the same time, but also affects the test of fosfomycin the accuracy of

Method used

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  • A method for simultaneously detecting fosfomycin and its diols by ion chromatography
  • A method for simultaneously detecting fosfomycin and its diols by ion chromatography
  • A method for simultaneously detecting fosfomycin and its diols by ion chromatography

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Embodiment 1

[0031] Detection of fosfomycin and its diols in fosfomycin pharmaceutical wastewater:

[0032] The column used is Dionex IonPac TM AS11-HC anion column (250×4mm, Dionex, USA), the suppressor used is ASRS300, and the ion chromatography parameters used are: injection volume 25μL, column temperature 30℃, flow rate 1.0mL / min, eluent 10mmol / min L of NaOH solution, suppressor current 25mA, analysis time of each sample is 60min.

[0033] Configure the solution of different concentrations (0.0025mmol / L-1.0mmol / L) with fosfomycin sodium, for obtaining the standard curve of fosfomycin; 0.02mmol / L-1.0mmol / L) to configure solutions of different concentrations for obtaining the standard curve of fosfomycin diol.

[0034] Take fosfomycin wastewater, pass through a 0.22 μm filter membrane and dilute 2000 times, take three 5mL ion chromatography injection bottles, add 5mL diluted samples respectively, as three parallel samples.

[0035] Run the ion chromatograph, and after the baseline is ...

Embodiment 2

[0046] With the KOH solution of 10mmol / L as eluent, all the other test conditions are identical with embodiment 1, and the mixed solution containing fosfomycin glycol and fosfomycin simultaneously of preparation is detected, by Figure 6 It can be seen that the peaks of the two substances do not affect each other, and the concentration of the two substances can be calculated according to the peak area.

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Abstract

The invention belongs to the technical field of chemical substance analysis and detection, and discloses a method for simultaneously detecting fosfomycin and its diols by using ion chromatography. The method that the present invention adopts ion chromatography to detect fosfomycin and its diols simultaneously comprises the following steps: (1) the selection of ion chromatography instrument and chromatographic test conditions: the chromatographic column is a low-capacity hydroxide system cathodic protection column, and the suppressor Adopt electrolytic membrane suppressor, eluent is NaOH or KOH solution; (2) draw fosfomycin standard curve with fosfomycin sodium as standard; (3) take fosfomycin trometamol EP impurity A as standard (4) test and obtain the peak area of ​​fosfomycin and its glycol in the sample, and obtain the corresponding concentration after substituting into the standard curve. The method of the invention has the advantages of good reproducibility, low detection limit, high sensitivity, simple operation, wide use of ion chromatographic equipment, etc., and can simultaneously test fosfomycin and its glycols.

Description

technical field [0001] The invention relates to the technical field of analysis and detection of chemical substances, in particular to a method for simultaneously detecting fosfomycin and its diols by using ion chromatography. Background technique [0002] In recent years, multidrug-resistant bacteria, pan-drug-resistant bacteria, and even full-drug-resistant bacteria have emerged continuously, which has brought severe challenges to the diagnosis and treatment of clinical infectious diseases. Studies have confirmed that fosfomycin has good antibacterial activity against drug-resistant bacteria due to its unique antibacterial mechanism. Therefore, fosfomycin has received great attention in clinical anti-infection treatment. However, in different stages of fosfomycin production, different concentrations of fosfomycin wastewater will be produced. As an antibiotic, fosfomycin is discharged into natural water bodies, which will cause drug resistance of bacteria in the environment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/96
CPCG01N30/96
Inventor 曾萍王良杰成璐瑶宋永会李娟
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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