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Biomarker and detection kit for early detection of severe hepatitis B virus, and application ofbiomarker

A biomarker and early detection technology, which is applied in the field of early detection of severe hepatitis B virus biomarkers and its detection kit, can solve the problems of reduced antioxidant capacity, reduced expression of GSTM3, and aggravated oxidative damage, so as to reduce related deaths rate effect

Pending Publication Date: 2021-04-30
SHANDONG UNIV
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hypermethylation of the GSTM3 gene promoter can reduce the expression of GSTM3, which in turn leads to a decrease in the body's antioxidant capacity, aggravated oxidative damage, and impaired normal liver function

Method used

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  • Biomarker and detection kit for early detection of severe hepatitis B virus, and application ofbiomarker
  • Biomarker and detection kit for early detection of severe hepatitis B virus, and application ofbiomarker
  • Biomarker and detection kit for early detection of severe hepatitis B virus, and application ofbiomarker

Examples

Experimental program
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Effect test

Embodiment 1

[0038]This study mainly collected peripheral venous blood from 50 patients with severe hepatitis B in Qilu Hospital of Shandong University.

[0039] Diagnostic criteria for severe hepatitis B: ① chronic hepatitis B history, positive HBsAg > 6 months; ② sudden severe jaundice with bilirubin rise ≥ 85mol / L and INR ≥ 1.5 (or PTA ≤ 40%); ③ ascites or hepatic encephalopathy.

[0040] Diagnostic criteria for chronic hepatitis B: ① HBsAg positive > 6 months; ② Serum HBV DNA > 20000IU / mL (105copies / mL), or 2000-20000IU / mL (104-105copies / mL) (common in HBeAg-negative CHB patients) ; ③ Persistent or intermittent elevated ALT / AST levels; ④ Liver biopsy showed chronic liver inflammation with moderate or severe necrosis;

[0041] Exclusion criteria: ① combined with other hepatitis virus infection (hepatitis A virus, hepatitis C virus, hepatitis D virus, hepatitis E virus, etc.) and / or human immunodeficiency virus; ② suffering from other liver diseases, such as autoimmune hepatitis, alcoho...

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Abstract

The invention relates to the technical field of biomedicine, particularly to a biomarker and a detection kit for early detection of severe hepatitis B virus, and application of the biomarker. The invention provides a biomarker for early detection of severe hepatitis B virus, wherein the sequence of the biomarker is shown as SEQ ID NO. 1. The invention also provides an application of the biomarker in preparation of a kit for early detection of severe hepatitis B virus, and a kit for early detection of severe hepatitis B. The kit comprises a primer pair and a probe for specific detection of GSTM3 methylation, and a primer pair and a probe for detecting a reference gene ALU-C4. The detection method disclosed by the invention can be used for detecting an extremely small amount of DNA in peripheral blood of a human body, is high in sensitivity and strong in specificity, and is widely applied to detection of specimens with low DNA content and high loss rate.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a biomarker for early detection of severe hepatitis B virus, a detection kit and application thereof. Background technique [0002] Severe hepatitis is a serious liver disease characterized by a large number of liver cell necrosis on the basis of chronic liver disease or cirrhosis. Its main manifestations are jaundice, coagulation dysfunction, hepatic encephalopathy and ascites, etc., with a high incidence of complications , is one of the leading causes of death in patients with liver disease. my country is a high prevalence area of ​​hepatitis B, and hepatitis B virus infection is the main cause of severe hepatitis in my country. Severe hepatitis B is in a dangerous condition, with poor prognosis and high fatality rate. At present, there is no effective method and means to evaluate the effect of clinical treatment in clinical practice. Therefore, there is an urgent need t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12N15/11C12R1/93
CPCC12Q1/706C12Q2600/154C12Q2600/166
Inventor 王凯向琳王婧雯范玉琛高帅
Owner SHANDONG UNIV
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