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Liquid-phase detection method for Pro-Xylane in cosmetics

A detection method, Bosein technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem that the Bosein content is rarely clearly marked, the Bosein concentration has not been published, and it is difficult to achieve effective Bosein content Detection and other problems, to achieve the effect of simple and fast pre-processing, high sensitivity and good accuracy

Active Publication Date: 2021-04-30
湖州中科院应用技术研究与产业化中心 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many kinds of cosmetics on the market, all of which claim to contain Boseine, but few can clearly label the content of Boseine
And even if there are products marked with the relevant concentration, it is only the concentration of the active substance contained in it, not the concentration of pure Boseine. For example, Helena, a product of L’Oreal, generally has a concentration of 30% of the active substance, but the actual content of the product is 30%. Contains unpublished concentration of Bosene
Therefore, how to quickly and effectively detect the actual content of Bosein in various skin care products is of great significance for the quality control of related products, but the existing detection methods are difficult to achieve effective detection of Bosein content in cosmetics

Method used

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  • Liquid-phase detection method for Pro-Xylane in cosmetics
  • Liquid-phase detection method for Pro-Xylane in cosmetics
  • Liquid-phase detection method for Pro-Xylane in cosmetics

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Effect test

Embodiment 1

[0027] This embodiment provides a liquid-phase detection method for Bosein in cosmetics, which includes:

[0028] (1) Preparation of standard curve:

[0029] Accurately weigh 50 mg of Bosein standard substance, prepare a 50 mg / ml standard stock solution with ultrapure water, and then double-dilute to 5 mg / mL, 10 mg / mL, 15 mg / mL, 20 mg / mL, 25 mg / mL, and use After HPLC-differential refraction detection method is detected, obtain as follows figure 1 In the chromatogram shown, wherein the chromatographic peak at the 5.4min place of retention time is boson, the concentration integration of the response peak area is obtained as figure 2 The standard curve shown: y=0.7273x, the correlation coefficient is 0.9963;

[0030] Wherein, the chromatographic condition of HPLC is: chromatographic column adopts Shodex Asahipak NH 2 P-50 4E, the mobile phase is 80% acetonitrile, isocratic elution, the detector is a differential refractive index detector, the injection volume is 10 μL, the co...

Embodiment 2

[0037] This embodiment provides a liquid-phase detection method for Bosein in cosmetics, which includes:

[0038] (2) Preparation of standard curve:

[0039] Accurately weigh 50 mg of Bosein standard substance, prepare a 50 mg / ml standard stock solution with ultrapure water, and then double-dilute to 5 mg / mL, 10 mg / mL, 15 mg / mL, 20 mg / mL, 25 mg / mL, and use After detection by HPLC-differential refraction detection method, the standard curve is obtained by integrating the concentration of the response peak area: y=0.7273x, and the correlation coefficient is 0.9963;

[0040] Among them, the chromatographic conditions of HPLC are: the chromatographic column adopts Shodex Asahipak NH2P-50 4E, the mobile phase is 75% acetonitrile, isocratic elution, the detector is a differential refractive index detector, the injection volume is 5 μL, and the column temperature is 72°C , the flow rate is 0.5mL / min;

[0041] (2) Sample preparation:

[0042] The raw material of Bosene obtained by ...

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Abstract

The invention provides a liquid-phase detection method for Pro-Xylane in cosmetics, and belongs to the field of cosmetic detection. The detection method is an HPLC-differential refraction detection method, and HPLC chromatographic conditions comprise that a chromatographic column adopts an amino column, a mobile phase is acetonitrile with the concentration of 75-85%, isocratic elution is performed, a detector is a differential refraction detector, and the sample size is 5-12 [mu]L. The method has the advantages of simple sample pretreatment, high detection sensitivity and wide linear range, and can effectively detect the content of the Pro-Xylane in various cosmetics.

Description

technical field [0001] The invention relates to the field of cosmetic detection, in particular to a liquid-phase detection method for bosonine in cosmetics. Background technique [0002] Bosein (Pro-Xylane), also known as hydroxypropyl tetrahydropyrantriol, is a biologically active substance that can promote the production of glycosaminoglycans (GAGs), thereby promoting the production of proteoglycans. At the same time, In this process, Bosein can also promote the synthesis of some cytokines and collagen regeneration through GAG, so that Bosein can resist skin aging, dehydration and other symptoms, and make the skin firm and elastic. In addition, some experiments have proved that Bosein can promote the regeneration of fibroblast growth factor (FGF, similar to EGF), and can effectively repair damaged skin. Therefore, Bosein is widely used in the field of cosmetics, mainly in high-end skin care products such as anti-wrinkle. [0003] At present, there are many kinds of cosme...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 王甜甜饶亮明李兴德
Owner 湖州中科院应用技术研究与产业化中心