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A liquid-phase detection method for bosonine in cosmetics

A detection method and technology of Boseine, applied in measuring devices, instruments, scientific instruments, etc., can solve the problem that it is difficult to achieve effective detection of Boseine content, the concentration of Boseine has not been published, and it is rarely possible to clearly indicate Boseine. Content and other issues, to achieve the effect of simple and fast pretreatment process, high sensitivity and good accuracy

Active Publication Date: 2022-05-24
湖州中科院应用技术研究与产业化中心 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are many kinds of cosmetics on the market, all of which claim to contain Boseine, but few can clearly label the content of Boseine
And even if there are products marked with the relevant concentration, it is only the concentration of the active substance contained in it, not the concentration of pure Boseine. For example, Helena, a product of L’Oreal, generally has a concentration of 30% of the active substance, but the actual content of the product is 30%. Contains unpublished concentration of Bosene
Therefore, how to quickly and effectively detect the actual content of Bosein in various skin care products is of great significance for the quality control of related products, but the existing detection methods are difficult to achieve effective detection of Bosein content in cosmetics

Method used

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  • A liquid-phase detection method for bosonine in cosmetics
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  • A liquid-phase detection method for bosonine in cosmetics

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Experimental program
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Embodiment 1

[0027] The present embodiment provides a liquid phase detection method of bosin in cosmetic products, comprising:

[0028] (1) Preparation of standard curves:

[0029] Accurately weigh Bosein standard substance 50mg, with ultrapure water formulated into 50mg / ml standard storage solution, and then double diluted to 5mg / mL, 10mg / mL, 15mg / mL, 20mg / mL, 25mg / mL, using HPLC - refractive index detection method for detection, such as Figure 1 The chromatogram shown, where the chromatographic peak at 5.4 min is Bosein, is obtained by integrating the concentration in response to the peak area Figure 2 Standard curve shown: y=0.7273x, correlation coefficient of 0.9963;

[0030] Among them, the chromatographic conditions of HPLC are: the column uses Shodex Asahipak NH 2P-50 4E, mobile phase is 80% acetonitrile, isometric elution, detector is a refractive index detector, the injection amount is 10 μL, the column temperature is 68 °C, and the flow rate is 0.8 mL / min;

[0031] (2) Sa...

Embodiment 2

[0037] The present embodiment provides a liquid phase detection method of bosin in cosmetic products, comprising:

[0038] (2) Preparation of standard curves:

[0039] Accurately weigh Bosein standard substance 50mg, use ultrapure water to make a standard storage solution of 50mg / ml, and then multiply dilute to 5mg / mL, 10mg / mL, 15mg / mL, 20mg / mL, 25mg / mL, after testing by HPLC-differential refractive indexing method, the standard curve is obtained by integrating the concentration of the response peak area: y=0.7273x, the correlation coefficient is 0.9963;

[0040] Among them, the chromatographic conditions of HPLC are: the column adopts Shodex Asahipak NH2P-50 4E, the mobile phase is 75% acetonitrile, isometric elution, the detector is a refractive index detector, the injection amount is 5μL, the column temperature is 72 °C, and the flow rate is 0.5mL / min;

[0041] (2) Sample preparation:

[0042] The bosein raw material obtained by chemical synthesis method is the No. 2 sample to ...

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Abstract

The invention provides a liquid-phase detection method for Bosein in cosmetics, which belongs to the field of cosmetic detection. This detection method is HPLC-differential refraction detection method, and the chromatographic condition of HPLC is: chromatographic column adopts amino post, and mobile phase is the acetonitrile of 75~85%, isocratic elution, and detector is differential refraction detector, and injection volume is 5-12 μL. The method has simple sample pretreatment, high detection sensitivity and wide linear range, and can effectively detect the content of bosonine in various cosmetics.

Description

Technical field [0001] The present invention relates to the field of cosmetic testing, in particular to a liquid phase detection method for bosein in cosmetics. Background [0002] Bosein (Pro-Xylane), also known as hydroxypropyl tetrahydropyrantriol, is a biologically active substance that can promote the production of glycosaminoglycans (GAG), which in turn promotes the production of proteoglycans, at the same time, bosein in this process, but also through GAG to promote the synthesis of some cytokines and collagen regeneration, so that Bosein has to fight skin aging, dehydration and other symptoms, so that the skin becomes firm and elastic. In addition, there are some experiments that have proved that Bose can promote the regeneration of fibroblast growth factor (FGF, similar to EGF) and can effectively repair damaged skin. Therefore, Bosein is widely used in the field of cosmetics, mainly used in high-end skin care products such as anti-wrinkle. [0003] At present, there are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 王甜甜饶亮明李兴德
Owner 湖州中科院应用技术研究与产业化中心
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