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A kind of room temperature phosphorescence detection method and application of sulfadimethoxine

A technology of sulfadimethoxine and sulfadimethoxine nucleic acid, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of complex detection process, high cost, large interference, etc., and achieve firm combination and mild synthesis conditions , The effect of simple detection method

Active Publication Date: 2022-03-18
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems that the existing sulfadimethoxine detection process is complex, time-consuming, high cost and large interference, the present invention provides a room temperature phosphorescence detection method and application of sulfadimethoxine

Method used

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  • A kind of room temperature phosphorescence detection method and application of sulfadimethoxine
  • A kind of room temperature phosphorescence detection method and application of sulfadimethoxine
  • A kind of room temperature phosphorescence detection method and application of sulfadimethoxine

Examples

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Effect test

Embodiment 1

[0036] Example 1, room temperature phosphorescence detection of sulfadimethoxine in river water

[0037] The first step is to prepare Mn:ZnS quantum dots:

[0038] Mercaptopropionic acid (MPA), Zn(Ac) 2 and Mn(Ac) 2 Mix according to the molar ratio of 4:1:0.05, adjust the pH value of the system to 11 with NaOH (2M), protect with nitrogen, and stir magnetically at room temperature for 30 minutes; then use a syringe to quickly add 90% Zn (Ac) 2 Na 2 S, continue to react at room temperature for 40 min; then heat the solution to 50 ° C, and age in air for 2 h to obtain the crude product of Mn-doped ZnS quantum dots coated with MPA. The same volume of absolute ethanol makes the quantum dots settle, centrifuges at high speed, pours off the supernatant, cleans it up three times, and dries in an oven at 50°C to obtain MPA-Mn:ZnS quantum dot solid powder;

[0039] The second step is to prepare MoS 2 Nanosheets:

[0040] Take 3mM (0.0181g) sodium molybdate dihydrate (H 4 MoNa 2...

Embodiment 2

[0053] Example 2, room temperature phosphorescence detection of sulfadimethoxine in river samples

[0054] The first step is to prepare Mn:ZnS quantum dots:

[0055] Mercaptopropionic acid (MPA), Zn(Ac) 2 and Mn(Ac) 2 Mix according to the molar ratio of 3.5:1:0.03, adjust the pH value of the system to 11 with NaOH (2M), protect with nitrogen, and stir magnetically at room temperature for 40 minutes; then use a syringe to quickly add 90% Zn (Ac) 2 Na 2 S, continue to react at room temperature for 40 min; then heat the solution to 50 ° C, and age in air for 3 h to obtain the crude product of Mn-doped ZnS quantum dots coated with MPA, which can be combined with the crude product of Mn-doped ZnS quantum dots coated with MPA The same volume of absolute ethanol makes the quantum dots settle, centrifuges at high speed, pours off the supernatant, cleans it up three times, and dries in an oven at 60°C to obtain MPA-Mn:ZnS quantum dot solid powder;

[0056] The second step is to pr...

Embodiment 3

[0071] Example 3, room temperature phosphorescence detection of sulfadimethoxine in milk

[0072] The first step is to prepare Mn:ZnS quantum dots:

[0073] Mercaptopropionic acid (MPA), Zn(Ac) 2 and Mn(Ac) 2 Mix according to the molar ratio of 4:1:0.04, adjust the pH value of the system to 11 with NaOH (2M), protect with nitrogen, and stir magnetically at room temperature for 30 minutes; then use a syringe to quickly add 90% Zn (Ac) 2 Na 2 S, continue to react at room temperature for 40min; then heat the solution to 60°C, and age in air for 3h to obtain the crude product of Mn-doped ZnS quantum dots coated with MPA. The same volume of absolute ethanol makes the quantum dots settle, centrifuges at high speed, pours off the supernatant, cleans it up three times, and dries in an oven at 60°C to obtain MPA-Mn:ZnS quantum dot solid powder;

[0074] The second step is to prepare MoS 2 Nanosheets:

[0075] Take 3mM (0.0181g) sodium molybdate dihydrate (H 4 MoNa 2 o 6 ) and...

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Abstract

The invention discloses a room temperature phosphorescence detection method and application of sulfadimethoxine, belonging to the technical field of detection of sulfadimethoxine. The invention solves the problems of complicated detection process, high cost and large interference of the existing sulfadimethoxine. The present invention utilizes prepared Mn:ZnS room temperature phosphorescent quantum dots (MPA-Mn:ZnS) modified with mercaptopropionic acid as phosphorescent probes, MoS 2 The nanosheet is the phosphorescence quenching unit, and the sulfadimethoxine nucleic acid aptamer is the molecular recognition unit. After the addition of sulfadimethoxine, the system is detected by MoS 2 The degree of recovery of room temperature phosphorescence quenched by nanosheets enables the analytical detection of sulfadimethoxine. The response range of the phosphorescence detection system to sulfadimethoxine is 2-400ng / mL, and the detection limit is 0.91ng / mL. It can be used for the detection of sulfadimethoxine in river water, milk and chicken. The generation of phosphorescence at room temperature does not require the addition of oxygen scavengers and inducers, and can avoid the interference of background fluorescence and scattered light of actual samples.

Description

technical field [0001] The invention belongs to the technical field of detection of sulfadimethoxine, and in particular relates to a room temperature phosphorescence detection method and application of sulfadimethoxine. Background technique [0002] Sulfadimethoxine (SDM) is a commonly used sulfonamide veterinary antibiotic, which has a broad-spectrum antibacterial effect and has strong pharmacological effects on coccidia and toxoplasma. It is widely used in the prevention and treatment of animal, poultry, and fish diseases, but it cannot be completely absorbed and metabolized when it enters the animal body, and it remains in the animal body or is excreted in the form of the parent compound and its metabolites through animal feces. As the drug resistance of germs becomes stronger and stronger, the dosage of antibiotics continues to increase, which will have a negative impact on the ecological balance, and may cause allergic reactions or hematopoietic system disorders in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 卫艳丽左力翔王丽董川
Owner SHANXI UNIV
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